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Immunopathological response of leukocytes against microfilariae and adult worms in white rats infected with Setaria cervi

AIM: Aim of this study was to see the immunopathological changes against the microfilariae (Mf) and adult worms of a bovine filarid, Setaria cervi in the tissues of vital organs of experimentally infected white rats. The effect of diethylcarbamazine (DEC) was also observed on the Mf, as leukocytes e...

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Autores principales: Kausar, Sharba, Khan, Wajihullah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465773/
https://www.ncbi.nlm.nih.gov/pubmed/28620263
http://dx.doi.org/10.14202/vetworld.2017.562-568
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author Kausar, Sharba
Khan, Wajihullah
author_facet Kausar, Sharba
Khan, Wajihullah
author_sort Kausar, Sharba
collection PubMed
description AIM: Aim of this study was to see the immunopathological changes against the microfilariae (Mf) and adult worms of a bovine filarid, Setaria cervi in the tissues of vital organs of experimentally infected white rats. The effect of diethylcarbamazine (DEC) was also observed on the Mf, as leukocytes especially lymphocytes produce immunoglobulins which opsonize and increase the efficacy of DEC against circulating Mf. Effect of this drug was also assessed on liver enzymes in the microfilaremic rats. MATERIALS AND METHODS: Microfilaremia was established by implanting adult worms intraperitoneally and by the infusion of Mf recovered from the uterus of gravid female worms. DEC was administered orally for six consecutive days in the rats having patent infection. Differential leukocyte count was recorded every 3(rd) day, and liver enzymes were estimated every 10(th) day in both treated and untreated rats. Pathological changes were observed in HE stained sections of vital organs where Mf or adult worms were trapped. RESULTS: Destruction and reduction in microfilarial density were noticed in microfilaremic rats treated with DEC. Trapped Mf and embedded worms revealed heavy cellular infiltrations by defensive cells which surrounded and attached with the body surface of the Mf as well as adult worms for their destruction and piece meal clearance. Immune-mediated pathology was observed in the tissue sections of lungs, spleen, and liver. Liver enzymes were elevated during the period of higher parasitemia. CONCLUSIONS: There was a moderate level of immunopathology against the Mf and adult worms by the leukocytes in experimentally infected microfilaremic rats. Mf were in the process of degeneration where they got trapped. Moderate increase in liver enzyme was noticed which was slightly more in untreated group. Although a fraction of Mf gets killed in the peritoneum, majority of them successfully enter the systemic circulation and survive for about 54 days, which is sufficient enough for conducting immunological and chemotherapeutic studies.
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spelling pubmed-54657732017-06-15 Immunopathological response of leukocytes against microfilariae and adult worms in white rats infected with Setaria cervi Kausar, Sharba Khan, Wajihullah Vet World Research Article AIM: Aim of this study was to see the immunopathological changes against the microfilariae (Mf) and adult worms of a bovine filarid, Setaria cervi in the tissues of vital organs of experimentally infected white rats. The effect of diethylcarbamazine (DEC) was also observed on the Mf, as leukocytes especially lymphocytes produce immunoglobulins which opsonize and increase the efficacy of DEC against circulating Mf. Effect of this drug was also assessed on liver enzymes in the microfilaremic rats. MATERIALS AND METHODS: Microfilaremia was established by implanting adult worms intraperitoneally and by the infusion of Mf recovered from the uterus of gravid female worms. DEC was administered orally for six consecutive days in the rats having patent infection. Differential leukocyte count was recorded every 3(rd) day, and liver enzymes were estimated every 10(th) day in both treated and untreated rats. Pathological changes were observed in HE stained sections of vital organs where Mf or adult worms were trapped. RESULTS: Destruction and reduction in microfilarial density were noticed in microfilaremic rats treated with DEC. Trapped Mf and embedded worms revealed heavy cellular infiltrations by defensive cells which surrounded and attached with the body surface of the Mf as well as adult worms for their destruction and piece meal clearance. Immune-mediated pathology was observed in the tissue sections of lungs, spleen, and liver. Liver enzymes were elevated during the period of higher parasitemia. CONCLUSIONS: There was a moderate level of immunopathology against the Mf and adult worms by the leukocytes in experimentally infected microfilaremic rats. Mf were in the process of degeneration where they got trapped. Moderate increase in liver enzyme was noticed which was slightly more in untreated group. Although a fraction of Mf gets killed in the peritoneum, majority of them successfully enter the systemic circulation and survive for about 54 days, which is sufficient enough for conducting immunological and chemotherapeutic studies. Veterinary World 2017-05 2017-05-30 /pmc/articles/PMC5465773/ /pubmed/28620263 http://dx.doi.org/10.14202/vetworld.2017.562-568 Text en Copyright: © Kausar and Khan. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Kausar, Sharba
Khan, Wajihullah
Immunopathological response of leukocytes against microfilariae and adult worms in white rats infected with Setaria cervi
title Immunopathological response of leukocytes against microfilariae and adult worms in white rats infected with Setaria cervi
title_full Immunopathological response of leukocytes against microfilariae and adult worms in white rats infected with Setaria cervi
title_fullStr Immunopathological response of leukocytes against microfilariae and adult worms in white rats infected with Setaria cervi
title_full_unstemmed Immunopathological response of leukocytes against microfilariae and adult worms in white rats infected with Setaria cervi
title_short Immunopathological response of leukocytes against microfilariae and adult worms in white rats infected with Setaria cervi
title_sort immunopathological response of leukocytes against microfilariae and adult worms in white rats infected with setaria cervi
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465773/
https://www.ncbi.nlm.nih.gov/pubmed/28620263
http://dx.doi.org/10.14202/vetworld.2017.562-568
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