Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration

We report the production of hGM-CSF cytokine in leaves of industrial tobacco cultivars DH-17 and DH-27 by using Agrobacterium-mediated transient expression. We prove the concept that very high biomass industrial tobacco plants are suitable platforms for rapid, low cost production of foreign proteins...

Descripción completa

Detalles Bibliográficos
Autores principales: Vojta, Lea, Ljuma-Skupnjak, Lana, Budimir, Ankica, Vukičević, Slobodan, Fulgosi, Hrvoje
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466047/
https://www.ncbi.nlm.nih.gov/pubmed/28626718
http://dx.doi.org/10.1016/j.btre.2015.05.006
_version_ 1783243022529462272
author Vojta, Lea
Ljuma-Skupnjak, Lana
Budimir, Ankica
Vukičević, Slobodan
Fulgosi, Hrvoje
author_facet Vojta, Lea
Ljuma-Skupnjak, Lana
Budimir, Ankica
Vukičević, Slobodan
Fulgosi, Hrvoje
author_sort Vojta, Lea
collection PubMed
description We report the production of hGM-CSF cytokine in leaves of industrial tobacco cultivars DH-17 and DH-27 by using Agrobacterium-mediated transient expression. We prove the concept that very high biomass industrial tobacco plants are suitable platforms for rapid, low cost production of foreign proteins. Successful transient expression of the GM-CSF was achieved in less than three months, opening the possibility for future applications of this approach in rapid response production of various proteins of non-plant origin in industrial tobacco.
format Online
Article
Text
id pubmed-5466047
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-54660472017-06-16 Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration Vojta, Lea Ljuma-Skupnjak, Lana Budimir, Ankica Vukičević, Slobodan Fulgosi, Hrvoje Biotechnol Rep (Amst) Article We report the production of hGM-CSF cytokine in leaves of industrial tobacco cultivars DH-17 and DH-27 by using Agrobacterium-mediated transient expression. We prove the concept that very high biomass industrial tobacco plants are suitable platforms for rapid, low cost production of foreign proteins. Successful transient expression of the GM-CSF was achieved in less than three months, opening the possibility for future applications of this approach in rapid response production of various proteins of non-plant origin in industrial tobacco. Elsevier 2015-05-30 /pmc/articles/PMC5466047/ /pubmed/28626718 http://dx.doi.org/10.1016/j.btre.2015.05.006 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Vojta, Lea
Ljuma-Skupnjak, Lana
Budimir, Ankica
Vukičević, Slobodan
Fulgosi, Hrvoje
Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration
title Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration
title_full Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration
title_fullStr Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration
title_full_unstemmed Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration
title_short Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration
title_sort rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (nicotiana tabacum l.) by agroinfiltration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466047/
https://www.ncbi.nlm.nih.gov/pubmed/28626718
http://dx.doi.org/10.1016/j.btre.2015.05.006
work_keys_str_mv AT vojtalea rapidtransientexpressionofhumangranulocytemacrophagecolonystimulatingfactorintwoindustrialcultivarsoftobacconicotianatabacumlbyagroinfiltration
AT ljumaskupnjaklana rapidtransientexpressionofhumangranulocytemacrophagecolonystimulatingfactorintwoindustrialcultivarsoftobacconicotianatabacumlbyagroinfiltration
AT budimirankica rapidtransientexpressionofhumangranulocytemacrophagecolonystimulatingfactorintwoindustrialcultivarsoftobacconicotianatabacumlbyagroinfiltration
AT vukicevicslobodan rapidtransientexpressionofhumangranulocytemacrophagecolonystimulatingfactorintwoindustrialcultivarsoftobacconicotianatabacumlbyagroinfiltration
AT fulgosihrvoje rapidtransientexpressionofhumangranulocytemacrophagecolonystimulatingfactorintwoindustrialcultivarsoftobacconicotianatabacumlbyagroinfiltration

Ejemplares similares