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Identification of Multipotent Stem Cells in Human Brain Tissue Following Stroke

Perivascular regions of the brain harbor multipotent stem cells. We previously demonstrated that brain pericytes near blood vessels also develop multipotency following experimental ischemia in mice and these ischemia-induced multipotent stem cells (iSCs) can contribute to neurogenesis. However, it i...

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Autores principales: Tatebayashi, Kotaro, Tanaka, Yasue, Nakano-Doi, Akiko, Sakuma, Rika, Kamachi, Saeko, Shirakawa, Manabu, Uchida, Kazutaka, Kageyama, Hiroto, Takagi, Toshinori, Yoshimura, Shinichi, Matsuyama, Tomohiro, Nakagomi, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466056/
https://www.ncbi.nlm.nih.gov/pubmed/28323540
http://dx.doi.org/10.1089/scd.2016.0334
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author Tatebayashi, Kotaro
Tanaka, Yasue
Nakano-Doi, Akiko
Sakuma, Rika
Kamachi, Saeko
Shirakawa, Manabu
Uchida, Kazutaka
Kageyama, Hiroto
Takagi, Toshinori
Yoshimura, Shinichi
Matsuyama, Tomohiro
Nakagomi, Takayuki
author_facet Tatebayashi, Kotaro
Tanaka, Yasue
Nakano-Doi, Akiko
Sakuma, Rika
Kamachi, Saeko
Shirakawa, Manabu
Uchida, Kazutaka
Kageyama, Hiroto
Takagi, Toshinori
Yoshimura, Shinichi
Matsuyama, Tomohiro
Nakagomi, Takayuki
author_sort Tatebayashi, Kotaro
collection PubMed
description Perivascular regions of the brain harbor multipotent stem cells. We previously demonstrated that brain pericytes near blood vessels also develop multipotency following experimental ischemia in mice and these ischemia-induced multipotent stem cells (iSCs) can contribute to neurogenesis. However, it is essential to understand the traits of iSCs in the poststroke human brain for possible applications in stem cell-based therapies for stroke patients. In this study, we report for the first time that iSCs can be isolated from the poststroke human brain. Putative iSCs were derived from poststroke brain tissue obtained from elderly stroke patients requiring decompressive craniectomy and partial lobectomy for diffuse cerebral infarction. Immunohistochemistry showed that these iSCs were localized near blood vessels within poststroke areas containing apoptotic/necrotic neurons and expressed both the stem cell marker nestin and several pericytic markers. Isolated iSCs expressed these same markers and demonstrated high proliferative potential without loss of stemness. Furthermore, isolated iSCs expressed other stem cell markers, such as Sox2, c-myc, and Klf4, and differentiated into multiple cells in vitro, including neurons. These results show that iSCs, which are likely brain pericyte derivatives, are present within the poststroke human brain. This study suggests that iSCs can contribute to neural repair in patients with stroke.
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spelling pubmed-54660562017-06-13 Identification of Multipotent Stem Cells in Human Brain Tissue Following Stroke Tatebayashi, Kotaro Tanaka, Yasue Nakano-Doi, Akiko Sakuma, Rika Kamachi, Saeko Shirakawa, Manabu Uchida, Kazutaka Kageyama, Hiroto Takagi, Toshinori Yoshimura, Shinichi Matsuyama, Tomohiro Nakagomi, Takayuki Stem Cells Dev Cutting-Edge Communication Perivascular regions of the brain harbor multipotent stem cells. We previously demonstrated that brain pericytes near blood vessels also develop multipotency following experimental ischemia in mice and these ischemia-induced multipotent stem cells (iSCs) can contribute to neurogenesis. However, it is essential to understand the traits of iSCs in the poststroke human brain for possible applications in stem cell-based therapies for stroke patients. In this study, we report for the first time that iSCs can be isolated from the poststroke human brain. Putative iSCs were derived from poststroke brain tissue obtained from elderly stroke patients requiring decompressive craniectomy and partial lobectomy for diffuse cerebral infarction. Immunohistochemistry showed that these iSCs were localized near blood vessels within poststroke areas containing apoptotic/necrotic neurons and expressed both the stem cell marker nestin and several pericytic markers. Isolated iSCs expressed these same markers and demonstrated high proliferative potential without loss of stemness. Furthermore, isolated iSCs expressed other stem cell markers, such as Sox2, c-myc, and Klf4, and differentiated into multiple cells in vitro, including neurons. These results show that iSCs, which are likely brain pericyte derivatives, are present within the poststroke human brain. This study suggests that iSCs can contribute to neural repair in patients with stroke. Mary Ann Liebert, Inc. 2017-06-01 2017-06-01 /pmc/articles/PMC5466056/ /pubmed/28323540 http://dx.doi.org/10.1089/scd.2016.0334 Text en © Kotaro Tatebayashi et al., 2017; Published by Mary Ann Liebert Inc. This article is available under the Creative Commons License CC-BY-NC (http://creativecommons.org/licenses/by-nc/4.0). This license permits non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. Permission only needs to be obtained for commercial use and can be done via RightsLink.
spellingShingle Cutting-Edge Communication
Tatebayashi, Kotaro
Tanaka, Yasue
Nakano-Doi, Akiko
Sakuma, Rika
Kamachi, Saeko
Shirakawa, Manabu
Uchida, Kazutaka
Kageyama, Hiroto
Takagi, Toshinori
Yoshimura, Shinichi
Matsuyama, Tomohiro
Nakagomi, Takayuki
Identification of Multipotent Stem Cells in Human Brain Tissue Following Stroke
title Identification of Multipotent Stem Cells in Human Brain Tissue Following Stroke
title_full Identification of Multipotent Stem Cells in Human Brain Tissue Following Stroke
title_fullStr Identification of Multipotent Stem Cells in Human Brain Tissue Following Stroke
title_full_unstemmed Identification of Multipotent Stem Cells in Human Brain Tissue Following Stroke
title_short Identification of Multipotent Stem Cells in Human Brain Tissue Following Stroke
title_sort identification of multipotent stem cells in human brain tissue following stroke
topic Cutting-Edge Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466056/
https://www.ncbi.nlm.nih.gov/pubmed/28323540
http://dx.doi.org/10.1089/scd.2016.0334
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