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Mechanism-based site-directed mutagenesis to shift the optimum pH of the phenylalanine ammonia-lyase from Rhodotorula glutinis JN-1

Phenylalanine ammonia-lyase (RgPAL) from Rhodotorula glutinis JN-1 stereoselectively catalyzes the conversion of the l-phenylalanine into trans-cinnamic acid and ammonia, and was used in chiral resolution of dl-phenylalanine to produce the d-phenylalanine under acidic condition. However, the optimum...

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Detalles Bibliográficos
Autores principales:	Zhu, Longbao, Zhou, Li, Cui, Wenjing, Liu, Zhongmei, Zhou, Zhemin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466100/ https://www.ncbi.nlm.nih.gov/pubmed/28626644 http://dx.doi.org/10.1016/j.btre.2014.06.001

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