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Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder

A modified twin-screw extruder incorporated with a filtration device was used as a liquid/solid separator for xylose removal from steam exploded corncobs. A face centered central composite design was used to study the combined effects of various enzymatic hydrolysis process variables (enzyme loading...

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Detalles Bibliográficos
Autores principales: Zheng, Jun, Choo, Kim, Bradt, Chris, Lehoux, Rick, Rehmann, Lars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466104/
https://www.ncbi.nlm.nih.gov/pubmed/28626653
http://dx.doi.org/10.1016/j.btre.2014.06.008
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author Zheng, Jun
Choo, Kim
Bradt, Chris
Lehoux, Rick
Rehmann, Lars
author_facet Zheng, Jun
Choo, Kim
Bradt, Chris
Lehoux, Rick
Rehmann, Lars
author_sort Zheng, Jun
collection PubMed
description A modified twin-screw extruder incorporated with a filtration device was used as a liquid/solid separator for xylose removal from steam exploded corncobs. A face centered central composite design was used to study the combined effects of various enzymatic hydrolysis process variables (enzyme loading, surfactant addition, and hydrolysis time) with two differently extruded corncobs (7% xylose removal, 80% xylose removal) on glucose conversion. The results showed that the extrusion process led to an increase in cellulose crystallinity, while structural changes could also be observed via SEM. A quadratic polynomial model was developed for predicting the glucose conversion and the fitted model provided an adequate approximation of the true response as verified by the analysis of variance (ANOVA).
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spelling pubmed-54661042017-06-16 Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder Zheng, Jun Choo, Kim Bradt, Chris Lehoux, Rick Rehmann, Lars Biotechnol Rep (Amst) Article A modified twin-screw extruder incorporated with a filtration device was used as a liquid/solid separator for xylose removal from steam exploded corncobs. A face centered central composite design was used to study the combined effects of various enzymatic hydrolysis process variables (enzyme loading, surfactant addition, and hydrolysis time) with two differently extruded corncobs (7% xylose removal, 80% xylose removal) on glucose conversion. The results showed that the extrusion process led to an increase in cellulose crystallinity, while structural changes could also be observed via SEM. A quadratic polynomial model was developed for predicting the glucose conversion and the fitted model provided an adequate approximation of the true response as verified by the analysis of variance (ANOVA). Elsevier 2014-06-26 /pmc/articles/PMC5466104/ /pubmed/28626653 http://dx.doi.org/10.1016/j.btre.2014.06.008 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Zheng, Jun
Choo, Kim
Bradt, Chris
Lehoux, Rick
Rehmann, Lars
Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder
title Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder
title_full Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder
title_fullStr Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder
title_full_unstemmed Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder
title_short Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder
title_sort enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466104/
https://www.ncbi.nlm.nih.gov/pubmed/28626653
http://dx.doi.org/10.1016/j.btre.2014.06.008
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