Cargando…
Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder
A modified twin-screw extruder incorporated with a filtration device was used as a liquid/solid separator for xylose removal from steam exploded corncobs. A face centered central composite design was used to study the combined effects of various enzymatic hydrolysis process variables (enzyme loading...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466104/ https://www.ncbi.nlm.nih.gov/pubmed/28626653 http://dx.doi.org/10.1016/j.btre.2014.06.008 |
_version_ | 1783243032625152000 |
---|---|
author | Zheng, Jun Choo, Kim Bradt, Chris Lehoux, Rick Rehmann, Lars |
author_facet | Zheng, Jun Choo, Kim Bradt, Chris Lehoux, Rick Rehmann, Lars |
author_sort | Zheng, Jun |
collection | PubMed |
description | A modified twin-screw extruder incorporated with a filtration device was used as a liquid/solid separator for xylose removal from steam exploded corncobs. A face centered central composite design was used to study the combined effects of various enzymatic hydrolysis process variables (enzyme loading, surfactant addition, and hydrolysis time) with two differently extruded corncobs (7% xylose removal, 80% xylose removal) on glucose conversion. The results showed that the extrusion process led to an increase in cellulose crystallinity, while structural changes could also be observed via SEM. A quadratic polynomial model was developed for predicting the glucose conversion and the fitted model provided an adequate approximation of the true response as verified by the analysis of variance (ANOVA). |
format | Online Article Text |
id | pubmed-5466104 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-54661042017-06-16 Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder Zheng, Jun Choo, Kim Bradt, Chris Lehoux, Rick Rehmann, Lars Biotechnol Rep (Amst) Article A modified twin-screw extruder incorporated with a filtration device was used as a liquid/solid separator for xylose removal from steam exploded corncobs. A face centered central composite design was used to study the combined effects of various enzymatic hydrolysis process variables (enzyme loading, surfactant addition, and hydrolysis time) with two differently extruded corncobs (7% xylose removal, 80% xylose removal) on glucose conversion. The results showed that the extrusion process led to an increase in cellulose crystallinity, while structural changes could also be observed via SEM. A quadratic polynomial model was developed for predicting the glucose conversion and the fitted model provided an adequate approximation of the true response as verified by the analysis of variance (ANOVA). Elsevier 2014-06-26 /pmc/articles/PMC5466104/ /pubmed/28626653 http://dx.doi.org/10.1016/j.btre.2014.06.008 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Article Zheng, Jun Choo, Kim Bradt, Chris Lehoux, Rick Rehmann, Lars Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder |
title | Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder |
title_full | Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder |
title_fullStr | Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder |
title_full_unstemmed | Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder |
title_short | Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder |
title_sort | enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466104/ https://www.ncbi.nlm.nih.gov/pubmed/28626653 http://dx.doi.org/10.1016/j.btre.2014.06.008 |
work_keys_str_mv | AT zhengjun enzymatichydrolysisofsteamexplodedcorncobresiduesafterpretreatmentinatwinscrewextruder AT chookim enzymatichydrolysisofsteamexplodedcorncobresiduesafterpretreatmentinatwinscrewextruder AT bradtchris enzymatichydrolysisofsteamexplodedcorncobresiduesafterpretreatmentinatwinscrewextruder AT lehouxrick enzymatichydrolysisofsteamexplodedcorncobresiduesafterpretreatmentinatwinscrewextruder AT rehmannlars enzymatichydrolysisofsteamexplodedcorncobresiduesafterpretreatmentinatwinscrewextruder |