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Evaluation of five in situ lysis protocols for PCR amenable metagenomic DNA from mangrove soils

Microbes in nature are rarely amenable to growth by standard microbiological methods, with the majority being unculturable. Metagenomic methods help to bypass and overcome the limitations of traditional culturing method; wherein total community DNA is isolated, cloned into suitable vector and host s...

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Detalles Bibliográficos
Autores principales: Nair, Harisree P., Vincent, Helvin, Bhat, Sarita G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466134/
https://www.ncbi.nlm.nih.gov/pubmed/28626672
http://dx.doi.org/10.1016/j.btre.2014.09.008