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Evaluation of five in situ lysis protocols for PCR amenable metagenomic DNA from mangrove soils
Microbes in nature are rarely amenable to growth by standard microbiological methods, with the majority being unculturable. Metagenomic methods help to bypass and overcome the limitations of traditional culturing method; wherein total community DNA is isolated, cloned into suitable vector and host s...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466134/ https://www.ncbi.nlm.nih.gov/pubmed/28626672 http://dx.doi.org/10.1016/j.btre.2014.09.008 |