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MATI, a Novel Protein Involved in the Regulation of Herbivore-Associated Signaling Pathways

The defense response of the plants against herbivores relies on a complex network of interconnected signaling pathways. In this work, we characterized a new key player in the response of Arabidopsis against the two-spotted spider mite Tetranychus urticae, the MATI (Mite Attack Triggered Immunity) ge...

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Autores principales: Santamaría, M. Estrella, Martinez, Manuel, Arnaiz, Ana, Ortego, Félix, Grbic, Vojislava, Diaz, Isabel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466143/
https://www.ncbi.nlm.nih.gov/pubmed/28649257
http://dx.doi.org/10.3389/fpls.2017.00975
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author Santamaría, M. Estrella
Martinez, Manuel
Arnaiz, Ana
Ortego, Félix
Grbic, Vojislava
Diaz, Isabel
author_facet Santamaría, M. Estrella
Martinez, Manuel
Arnaiz, Ana
Ortego, Félix
Grbic, Vojislava
Diaz, Isabel
author_sort Santamaría, M. Estrella
collection PubMed
description The defense response of the plants against herbivores relies on a complex network of interconnected signaling pathways. In this work, we characterized a new key player in the response of Arabidopsis against the two-spotted spider mite Tetranychus urticae, the MATI (Mite Attack Triggered Immunity) gene. This gene was differentially induced in resistant Bla-2 strain relative to susceptible Kon Arabidopsis accessions after mite attack, suggesting a potential role in the control of spider mites. To study the MATI gene function, it has been performed a deep molecular characterization of the gene combined with feeding bioassays using modified Arabidopsis lines and phytophagous arthropods. The MATI gene belongs to a new gene family that had not been previously characterized. Biotic assays showed that it confers a high tolerance not only to T. urticae, but also to the chewing lepidopteran Spodoptera exigua. Biochemical analyses suggest that MATI encodes a protein involved in the accumulation of reducing agents upon herbivore attack to control plant redox homeostasis avoiding oxidative damage and cell death. Besides, molecular analyses demonstrated that MATI is involved in the modulation of different hormonal signaling pathways, affecting the expression of genes involved in biosynthesis and signaling of the jasmonic acid and salicylic acid hormones. The fact that MATI is also involved in defense through the modulation of the levels of photosynthetic pigments highlights the potential of MATI proteins to be exploited as biotechnological tools for pest control.
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spelling pubmed-54661432017-06-23 MATI, a Novel Protein Involved in the Regulation of Herbivore-Associated Signaling Pathways Santamaría, M. Estrella Martinez, Manuel Arnaiz, Ana Ortego, Félix Grbic, Vojislava Diaz, Isabel Front Plant Sci Plant Science The defense response of the plants against herbivores relies on a complex network of interconnected signaling pathways. In this work, we characterized a new key player in the response of Arabidopsis against the two-spotted spider mite Tetranychus urticae, the MATI (Mite Attack Triggered Immunity) gene. This gene was differentially induced in resistant Bla-2 strain relative to susceptible Kon Arabidopsis accessions after mite attack, suggesting a potential role in the control of spider mites. To study the MATI gene function, it has been performed a deep molecular characterization of the gene combined with feeding bioassays using modified Arabidopsis lines and phytophagous arthropods. The MATI gene belongs to a new gene family that had not been previously characterized. Biotic assays showed that it confers a high tolerance not only to T. urticae, but also to the chewing lepidopteran Spodoptera exigua. Biochemical analyses suggest that MATI encodes a protein involved in the accumulation of reducing agents upon herbivore attack to control plant redox homeostasis avoiding oxidative damage and cell death. Besides, molecular analyses demonstrated that MATI is involved in the modulation of different hormonal signaling pathways, affecting the expression of genes involved in biosynthesis and signaling of the jasmonic acid and salicylic acid hormones. The fact that MATI is also involved in defense through the modulation of the levels of photosynthetic pigments highlights the potential of MATI proteins to be exploited as biotechnological tools for pest control. Frontiers Media S.A. 2017-06-09 /pmc/articles/PMC5466143/ /pubmed/28649257 http://dx.doi.org/10.3389/fpls.2017.00975 Text en Copyright © 2017 Santamaría, Martinez, Arnaiz, Ortego, Grbic and Diaz. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Santamaría, M. Estrella
Martinez, Manuel
Arnaiz, Ana
Ortego, Félix
Grbic, Vojislava
Diaz, Isabel
MATI, a Novel Protein Involved in the Regulation of Herbivore-Associated Signaling Pathways
title MATI, a Novel Protein Involved in the Regulation of Herbivore-Associated Signaling Pathways
title_full MATI, a Novel Protein Involved in the Regulation of Herbivore-Associated Signaling Pathways
title_fullStr MATI, a Novel Protein Involved in the Regulation of Herbivore-Associated Signaling Pathways
title_full_unstemmed MATI, a Novel Protein Involved in the Regulation of Herbivore-Associated Signaling Pathways
title_short MATI, a Novel Protein Involved in the Regulation of Herbivore-Associated Signaling Pathways
title_sort mati, a novel protein involved in the regulation of herbivore-associated signaling pathways
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466143/
https://www.ncbi.nlm.nih.gov/pubmed/28649257
http://dx.doi.org/10.3389/fpls.2017.00975
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