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Highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Swertia lawii Burkill

Highly efficient in vitro regeneration system has been developed for Swertia lawii Burkill, an important herb used as substitute for Swertia chirayita. Shoot tips explants were cultured on MS medium with various phytohormones for multiple shoot production. The best shoot production frequency (100%)...

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Detalles Bibliográficos
Autores principales: Kshirsagar, Parthraj R., Chavan, Jaykumar J., Umdale, Suraj D., Nimbalkar, Mansingraj S., Dixit, Ghansham B., Gaikwad, Nikhil B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466263/
https://www.ncbi.nlm.nih.gov/pubmed/28626699
http://dx.doi.org/10.1016/j.btre.2015.03.003
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author Kshirsagar, Parthraj R.
Chavan, Jaykumar J.
Umdale, Suraj D.
Nimbalkar, Mansingraj S.
Dixit, Ghansham B.
Gaikwad, Nikhil B.
author_facet Kshirsagar, Parthraj R.
Chavan, Jaykumar J.
Umdale, Suraj D.
Nimbalkar, Mansingraj S.
Dixit, Ghansham B.
Gaikwad, Nikhil B.
author_sort Kshirsagar, Parthraj R.
collection PubMed
description Highly efficient in vitro regeneration system has been developed for Swertia lawii Burkill, an important herb used as substitute for Swertia chirayita. Shoot tips explants were cultured on MS medium with various phytohormones for multiple shoot production. The best shoot production frequency (100%) and maximum shoots (10.4 ± 0.8) were obtained on MS media containing TDZ (3.0 mg l(−1)) in combination with IBA (0.3 mg l(−1)). Maximum callus induction (95 ± 4.8%) and callus growth (1.7 ± 0.4 gm) was achieved on MS medium with 2, 4-D (3.0 mg l(−1)). Cell suspension cultures were established and studied for their growth kinetics. Shoots were rooted best (22.1 ± 2.5) in 1/2 MS medium with IAA (3.0 mg l(−1)). The genetic uniformity of the micropropagated clones was assessed using RAPD markers. Out of 405 bands, 400 (98.76%) were monomorphic and rest 5 (1.24%) were polymorphic. High multiplication frequency and low risk of genetic instability ensures the efficacy of this protocol.
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spelling pubmed-54662632017-06-16 Highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Swertia lawii Burkill Kshirsagar, Parthraj R. Chavan, Jaykumar J. Umdale, Suraj D. Nimbalkar, Mansingraj S. Dixit, Ghansham B. Gaikwad, Nikhil B. Biotechnol Rep (Amst) Article Highly efficient in vitro regeneration system has been developed for Swertia lawii Burkill, an important herb used as substitute for Swertia chirayita. Shoot tips explants were cultured on MS medium with various phytohormones for multiple shoot production. The best shoot production frequency (100%) and maximum shoots (10.4 ± 0.8) were obtained on MS media containing TDZ (3.0 mg l(−1)) in combination with IBA (0.3 mg l(−1)). Maximum callus induction (95 ± 4.8%) and callus growth (1.7 ± 0.4 gm) was achieved on MS medium with 2, 4-D (3.0 mg l(−1)). Cell suspension cultures were established and studied for their growth kinetics. Shoots were rooted best (22.1 ± 2.5) in 1/2 MS medium with IAA (3.0 mg l(−1)). The genetic uniformity of the micropropagated clones was assessed using RAPD markers. Out of 405 bands, 400 (98.76%) were monomorphic and rest 5 (1.24%) were polymorphic. High multiplication frequency and low risk of genetic instability ensures the efficacy of this protocol. Elsevier 2015-03-11 /pmc/articles/PMC5466263/ /pubmed/28626699 http://dx.doi.org/10.1016/j.btre.2015.03.003 Text en © 2015 Published by Elsevier B.V. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kshirsagar, Parthraj R.
Chavan, Jaykumar J.
Umdale, Suraj D.
Nimbalkar, Mansingraj S.
Dixit, Ghansham B.
Gaikwad, Nikhil B.
Highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Swertia lawii Burkill
title Highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Swertia lawii Burkill
title_full Highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Swertia lawii Burkill
title_fullStr Highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Swertia lawii Burkill
title_full_unstemmed Highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Swertia lawii Burkill
title_short Highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Swertia lawii Burkill
title_sort highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and rapd analysis of regenerants of swertia lawii burkill
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466263/
https://www.ncbi.nlm.nih.gov/pubmed/28626699
http://dx.doi.org/10.1016/j.btre.2015.03.003
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