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Global gene expression defines faded whorl specification of double flower domestication in Camellia
Double flowers in cultivated camellias are divergent in floral patterns which present a rich resource for demonstrating molecular modifications influenced by the human demands. Despite the key principle of ABCE model in whorl specification, the underlying mechanism of fine-tuning double flower forma...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466612/ https://www.ncbi.nlm.nih.gov/pubmed/28600507 http://dx.doi.org/10.1038/s41598-017-03575-2 |
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author | Li, Xinlei Li, Jiyuan Fan, Zhengqi Liu, Zhongchi Tanaka, Takayuki Yin, Hengfu |
author_facet | Li, Xinlei Li, Jiyuan Fan, Zhengqi Liu, Zhongchi Tanaka, Takayuki Yin, Hengfu |
author_sort | Li, Xinlei |
collection | PubMed |
description | Double flowers in cultivated camellias are divergent in floral patterns which present a rich resource for demonstrating molecular modifications influenced by the human demands. Despite the key principle of ABCE model in whorl specification, the underlying mechanism of fine-tuning double flower formation remains largely unclear. Here a comprehensive comparative transcriptomics interrogation of gene expression among floral organs of wild type and “formal double” and “anemone double” is presented. Through a combination of transcriptome, small RNA and “degradome” sequencing, we studied the regulatory gene expression network underlying the double flower formation. We obtained the differentially expressed genes between whorls in wild and cultivated Camellia. We showed that the formation of double flowers tends to demolish gene expression canalization of key functions; the faded whorl specification mechanism was fundamental under the diverse patterns of double flowers. Furthermore, we identified conserved miRNA-targets regulations in the control of double flowers, and we found that miR172-AP2, miR156-SPLs were critical regulatory nodes contributing to the diversity of double flower forms. This work highlights the hierarchical patterning of global gene expression in floral development, and supports the roles of “faded ABC model” mechanism and miRNA-targets regulations underlying the double flower domestication. |
format | Online Article Text |
id | pubmed-5466612 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54666122017-06-14 Global gene expression defines faded whorl specification of double flower domestication in Camellia Li, Xinlei Li, Jiyuan Fan, Zhengqi Liu, Zhongchi Tanaka, Takayuki Yin, Hengfu Sci Rep Article Double flowers in cultivated camellias are divergent in floral patterns which present a rich resource for demonstrating molecular modifications influenced by the human demands. Despite the key principle of ABCE model in whorl specification, the underlying mechanism of fine-tuning double flower formation remains largely unclear. Here a comprehensive comparative transcriptomics interrogation of gene expression among floral organs of wild type and “formal double” and “anemone double” is presented. Through a combination of transcriptome, small RNA and “degradome” sequencing, we studied the regulatory gene expression network underlying the double flower formation. We obtained the differentially expressed genes between whorls in wild and cultivated Camellia. We showed that the formation of double flowers tends to demolish gene expression canalization of key functions; the faded whorl specification mechanism was fundamental under the diverse patterns of double flowers. Furthermore, we identified conserved miRNA-targets regulations in the control of double flowers, and we found that miR172-AP2, miR156-SPLs were critical regulatory nodes contributing to the diversity of double flower forms. This work highlights the hierarchical patterning of global gene expression in floral development, and supports the roles of “faded ABC model” mechanism and miRNA-targets regulations underlying the double flower domestication. Nature Publishing Group UK 2017-06-09 /pmc/articles/PMC5466612/ /pubmed/28600507 http://dx.doi.org/10.1038/s41598-017-03575-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Li, Xinlei Li, Jiyuan Fan, Zhengqi Liu, Zhongchi Tanaka, Takayuki Yin, Hengfu Global gene expression defines faded whorl specification of double flower domestication in Camellia |
title | Global gene expression defines faded whorl specification of double flower domestication in Camellia |
title_full | Global gene expression defines faded whorl specification of double flower domestication in Camellia |
title_fullStr | Global gene expression defines faded whorl specification of double flower domestication in Camellia |
title_full_unstemmed | Global gene expression defines faded whorl specification of double flower domestication in Camellia |
title_short | Global gene expression defines faded whorl specification of double flower domestication in Camellia |
title_sort | global gene expression defines faded whorl specification of double flower domestication in camellia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466612/ https://www.ncbi.nlm.nih.gov/pubmed/28600507 http://dx.doi.org/10.1038/s41598-017-03575-2 |
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