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Global gene expression defines faded whorl specification of double flower domestication in Camellia

Double flowers in cultivated camellias are divergent in floral patterns which present a rich resource for demonstrating molecular modifications influenced by the human demands. Despite the key principle of ABCE model in whorl specification, the underlying mechanism of fine-tuning double flower forma...

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Autores principales: Li, Xinlei, Li, Jiyuan, Fan, Zhengqi, Liu, Zhongchi, Tanaka, Takayuki, Yin, Hengfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466612/
https://www.ncbi.nlm.nih.gov/pubmed/28600507
http://dx.doi.org/10.1038/s41598-017-03575-2
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author Li, Xinlei
Li, Jiyuan
Fan, Zhengqi
Liu, Zhongchi
Tanaka, Takayuki
Yin, Hengfu
author_facet Li, Xinlei
Li, Jiyuan
Fan, Zhengqi
Liu, Zhongchi
Tanaka, Takayuki
Yin, Hengfu
author_sort Li, Xinlei
collection PubMed
description Double flowers in cultivated camellias are divergent in floral patterns which present a rich resource for demonstrating molecular modifications influenced by the human demands. Despite the key principle of ABCE model in whorl specification, the underlying mechanism of fine-tuning double flower formation remains largely unclear. Here a comprehensive comparative transcriptomics interrogation of gene expression among floral organs of wild type and “formal double” and “anemone double” is presented. Through a combination of transcriptome, small RNA and “degradome” sequencing, we studied the regulatory gene expression network underlying the double flower formation. We obtained the differentially expressed genes between whorls in wild and cultivated Camellia. We showed that the formation of double flowers tends to demolish gene expression canalization of key functions; the faded whorl specification mechanism was fundamental under the diverse patterns of double flowers. Furthermore, we identified conserved miRNA-targets regulations in the control of double flowers, and we found that miR172-AP2, miR156-SPLs were critical regulatory nodes contributing to the diversity of double flower forms. This work highlights the hierarchical patterning of global gene expression in floral development, and supports the roles of “faded ABC model” mechanism and miRNA-targets regulations underlying the double flower domestication.
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spelling pubmed-54666122017-06-14 Global gene expression defines faded whorl specification of double flower domestication in Camellia Li, Xinlei Li, Jiyuan Fan, Zhengqi Liu, Zhongchi Tanaka, Takayuki Yin, Hengfu Sci Rep Article Double flowers in cultivated camellias are divergent in floral patterns which present a rich resource for demonstrating molecular modifications influenced by the human demands. Despite the key principle of ABCE model in whorl specification, the underlying mechanism of fine-tuning double flower formation remains largely unclear. Here a comprehensive comparative transcriptomics interrogation of gene expression among floral organs of wild type and “formal double” and “anemone double” is presented. Through a combination of transcriptome, small RNA and “degradome” sequencing, we studied the regulatory gene expression network underlying the double flower formation. We obtained the differentially expressed genes between whorls in wild and cultivated Camellia. We showed that the formation of double flowers tends to demolish gene expression canalization of key functions; the faded whorl specification mechanism was fundamental under the diverse patterns of double flowers. Furthermore, we identified conserved miRNA-targets regulations in the control of double flowers, and we found that miR172-AP2, miR156-SPLs were critical regulatory nodes contributing to the diversity of double flower forms. This work highlights the hierarchical patterning of global gene expression in floral development, and supports the roles of “faded ABC model” mechanism and miRNA-targets regulations underlying the double flower domestication. Nature Publishing Group UK 2017-06-09 /pmc/articles/PMC5466612/ /pubmed/28600507 http://dx.doi.org/10.1038/s41598-017-03575-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Xinlei
Li, Jiyuan
Fan, Zhengqi
Liu, Zhongchi
Tanaka, Takayuki
Yin, Hengfu
Global gene expression defines faded whorl specification of double flower domestication in Camellia
title Global gene expression defines faded whorl specification of double flower domestication in Camellia
title_full Global gene expression defines faded whorl specification of double flower domestication in Camellia
title_fullStr Global gene expression defines faded whorl specification of double flower domestication in Camellia
title_full_unstemmed Global gene expression defines faded whorl specification of double flower domestication in Camellia
title_short Global gene expression defines faded whorl specification of double flower domestication in Camellia
title_sort global gene expression defines faded whorl specification of double flower domestication in camellia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466612/
https://www.ncbi.nlm.nih.gov/pubmed/28600507
http://dx.doi.org/10.1038/s41598-017-03575-2
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