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Induction of sirtuin-1 signaling by resveratrol induces human chondrosarcoma cell apoptosis and exhibits antitumor activity

Chondrosarcoma is a malignant primary bone tumor. Sirtuin-1 (SIRT1), which is a member of sirtuin family, plays a dual role either in cancer promotion or suppression. There is no report about the role of SIRT1 in the human chondrosarcoma cells. Resveratrol is a potent activator of SIRT1. However, it...

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Autores principales: Chao, Sung-Chuan, Chen, Ying-Ju, Huang, Kuo-How, Kuo, Kuan-Lin, Yang, Ting-Hua, Huang, Kuo-Yuan, Wang, Ching-Chia, Tang, Chih-Hsin, Yang, Rong-Sen, Liu, Shing-Hwa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466619/
https://www.ncbi.nlm.nih.gov/pubmed/28600541
http://dx.doi.org/10.1038/s41598-017-03635-7
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author Chao, Sung-Chuan
Chen, Ying-Ju
Huang, Kuo-How
Kuo, Kuan-Lin
Yang, Ting-Hua
Huang, Kuo-Yuan
Wang, Ching-Chia
Tang, Chih-Hsin
Yang, Rong-Sen
Liu, Shing-Hwa
author_facet Chao, Sung-Chuan
Chen, Ying-Ju
Huang, Kuo-How
Kuo, Kuan-Lin
Yang, Ting-Hua
Huang, Kuo-Yuan
Wang, Ching-Chia
Tang, Chih-Hsin
Yang, Rong-Sen
Liu, Shing-Hwa
author_sort Chao, Sung-Chuan
collection PubMed
description Chondrosarcoma is a malignant primary bone tumor. Sirtuin-1 (SIRT1), which is a member of sirtuin family, plays a dual role either in cancer promotion or suppression. There is no report about the role of SIRT1 in the human chondrosarcoma cells. Resveratrol is a potent activator of SIRT1. However, its effects on chondrosarcoma have not been extensively studied. Here, we investigated the role of SIRT1 induction by resveratrol in human chondrosarcoma cell growth and tumor progression. Resveratrol significantly decreased cell viability and induced cell apoptosis in human chondrosarcoma cells in a dose-dependent manner. The protein expression and activity of SIRT1 were activated after treatment with resveratrol. Resveratrol significantly inhibited NF-κB signaling by deacetylating the p65 subunit of NF-κB complex, which could be reversed by siRNA-SIRT1 transfection or deacetylation inhibitor MS-275. Resveratrol induced-apoptosis involved a caspase-3-mediated mechanism. Both siRNA-SIRT1 transfection and MS-275 significantly inhibited the resveratrol-induced caspase-3 cleavage and activity in human chondrosarcoma cells. Moreover, in vivo chondrosarcoma xenograft study revealed a dramatic reduction in tumor volume and the increased SIRT1 and cleaved caspase-3 expressions in tumors by resveratrol treatment. These results suggest that resveratrol induces chondrosarcoma cell apoptosis via a SIRT1-activated NF-κB deacetylation and exhibits anti-chondrosarcoma activity in vivo.
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spelling pubmed-54666192017-06-14 Induction of sirtuin-1 signaling by resveratrol induces human chondrosarcoma cell apoptosis and exhibits antitumor activity Chao, Sung-Chuan Chen, Ying-Ju Huang, Kuo-How Kuo, Kuan-Lin Yang, Ting-Hua Huang, Kuo-Yuan Wang, Ching-Chia Tang, Chih-Hsin Yang, Rong-Sen Liu, Shing-Hwa Sci Rep Article Chondrosarcoma is a malignant primary bone tumor. Sirtuin-1 (SIRT1), which is a member of sirtuin family, plays a dual role either in cancer promotion or suppression. There is no report about the role of SIRT1 in the human chondrosarcoma cells. Resveratrol is a potent activator of SIRT1. However, its effects on chondrosarcoma have not been extensively studied. Here, we investigated the role of SIRT1 induction by resveratrol in human chondrosarcoma cell growth and tumor progression. Resveratrol significantly decreased cell viability and induced cell apoptosis in human chondrosarcoma cells in a dose-dependent manner. The protein expression and activity of SIRT1 were activated after treatment with resveratrol. Resveratrol significantly inhibited NF-κB signaling by deacetylating the p65 subunit of NF-κB complex, which could be reversed by siRNA-SIRT1 transfection or deacetylation inhibitor MS-275. Resveratrol induced-apoptosis involved a caspase-3-mediated mechanism. Both siRNA-SIRT1 transfection and MS-275 significantly inhibited the resveratrol-induced caspase-3 cleavage and activity in human chondrosarcoma cells. Moreover, in vivo chondrosarcoma xenograft study revealed a dramatic reduction in tumor volume and the increased SIRT1 and cleaved caspase-3 expressions in tumors by resveratrol treatment. These results suggest that resveratrol induces chondrosarcoma cell apoptosis via a SIRT1-activated NF-κB deacetylation and exhibits anti-chondrosarcoma activity in vivo. Nature Publishing Group UK 2017-06-09 /pmc/articles/PMC5466619/ /pubmed/28600541 http://dx.doi.org/10.1038/s41598-017-03635-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chao, Sung-Chuan
Chen, Ying-Ju
Huang, Kuo-How
Kuo, Kuan-Lin
Yang, Ting-Hua
Huang, Kuo-Yuan
Wang, Ching-Chia
Tang, Chih-Hsin
Yang, Rong-Sen
Liu, Shing-Hwa
Induction of sirtuin-1 signaling by resveratrol induces human chondrosarcoma cell apoptosis and exhibits antitumor activity
title Induction of sirtuin-1 signaling by resveratrol induces human chondrosarcoma cell apoptosis and exhibits antitumor activity
title_full Induction of sirtuin-1 signaling by resveratrol induces human chondrosarcoma cell apoptosis and exhibits antitumor activity
title_fullStr Induction of sirtuin-1 signaling by resveratrol induces human chondrosarcoma cell apoptosis and exhibits antitumor activity
title_full_unstemmed Induction of sirtuin-1 signaling by resveratrol induces human chondrosarcoma cell apoptosis and exhibits antitumor activity
title_short Induction of sirtuin-1 signaling by resveratrol induces human chondrosarcoma cell apoptosis and exhibits antitumor activity
title_sort induction of sirtuin-1 signaling by resveratrol induces human chondrosarcoma cell apoptosis and exhibits antitumor activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466619/
https://www.ncbi.nlm.nih.gov/pubmed/28600541
http://dx.doi.org/10.1038/s41598-017-03635-7
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