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Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities

Nano/microfibrous structure can induce high cellular activities because of the topological similarity of the extracellular matrix, and thus, are widely used in various tissue regenerative materials. However, the fabrication of a bioceramic (high weight percent)-based 3D microfibrous structure is ext...

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Autores principales: Kim, Minseong, Yun, Hui-suk, Kim, Geun Hyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466689/
https://www.ncbi.nlm.nih.gov/pubmed/28600540
http://dx.doi.org/10.1038/s41598-017-03461-x
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author Kim, Minseong
Yun, Hui-suk
Kim, Geun Hyung
author_facet Kim, Minseong
Yun, Hui-suk
Kim, Geun Hyung
author_sort Kim, Minseong
collection PubMed
description Nano/microfibrous structure can induce high cellular activities because of the topological similarity of the extracellular matrix, and thus, are widely used in various tissue regenerative materials. However, the fabrication of a bioceramic (high weight percent)-based 3D microfibrous structure is extremely difficult because of the low process-ability of bioceramics. In addition, three-dimensional (3D) microfibrous structure can induce more realistic cellular behavior when compared to that of 2D fibrous structure. Hence, the requirement of a 3D fibrous ceramic-based structure is an important issue in bioceramic scaffolds. In this study, a bioceramic (α-TCP)-based scaffold in which the weight fraction of the ceramic exceeded 70% was fabricated using an electrohydrodynamic printing (EHDP) process. The fabricated ceramic structure consisted of layer-by-layered struts entangled with polycaprolactone microfibers and the bioceramic phase. Various processing conditions (such as applied electric field, flow rate, nozzle size, and weight fraction of the bioceramic) were manipulated to obtain an optimal processing window. A 3D printed porous structure was used as a control, which had pore geometry similar to that of a structure fabricated using the EHDP process. Various physical and cellular activities using preosteoblasts (MC3T3-E1) helped confirm that the newly designed bioceramic scaffold demonstrated significantly high metabolic activity and mineralization.
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spelling pubmed-54666892017-06-14 Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities Kim, Minseong Yun, Hui-suk Kim, Geun Hyung Sci Rep Article Nano/microfibrous structure can induce high cellular activities because of the topological similarity of the extracellular matrix, and thus, are widely used in various tissue regenerative materials. However, the fabrication of a bioceramic (high weight percent)-based 3D microfibrous structure is extremely difficult because of the low process-ability of bioceramics. In addition, three-dimensional (3D) microfibrous structure can induce more realistic cellular behavior when compared to that of 2D fibrous structure. Hence, the requirement of a 3D fibrous ceramic-based structure is an important issue in bioceramic scaffolds. In this study, a bioceramic (α-TCP)-based scaffold in which the weight fraction of the ceramic exceeded 70% was fabricated using an electrohydrodynamic printing (EHDP) process. The fabricated ceramic structure consisted of layer-by-layered struts entangled with polycaprolactone microfibers and the bioceramic phase. Various processing conditions (such as applied electric field, flow rate, nozzle size, and weight fraction of the bioceramic) were manipulated to obtain an optimal processing window. A 3D printed porous structure was used as a control, which had pore geometry similar to that of a structure fabricated using the EHDP process. Various physical and cellular activities using preosteoblasts (MC3T3-E1) helped confirm that the newly designed bioceramic scaffold demonstrated significantly high metabolic activity and mineralization. Nature Publishing Group UK 2017-06-09 /pmc/articles/PMC5466689/ /pubmed/28600540 http://dx.doi.org/10.1038/s41598-017-03461-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kim, Minseong
Yun, Hui-suk
Kim, Geun Hyung
Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities
title Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities
title_full Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities
title_fullStr Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities
title_full_unstemmed Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities
title_short Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities
title_sort electric-field assisted 3d-fibrous bioceramic-based scaffolds for bone tissue regeneration: fabrication, characterization, and in vitro cellular activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466689/
https://www.ncbi.nlm.nih.gov/pubmed/28600540
http://dx.doi.org/10.1038/s41598-017-03461-x
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