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Characterization of Schistosoma japonicum tetraspanning orphan receptor and its role in binding to complement C2 and immunoprotection against murine schistosomiasis

BACKGROUND: Schistosomiasis remains an important global public health problem, as millions of people are at risk of acquiring infection. An ideal method for sustainable control of schistosomiasis would be to develop an efficient vaccine. Schistosomes can survive in the host vascular system by immune...

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Autores principales: Ma, Shuai, Zai, Jinli, Han, Yanhui, Hong, Yang, Zhang, Min, Cao, Xiaodan, Han, Qian, Lu, Ke, Zhao, Zhixin, Lin, Jiaojiao, Fu, Zhiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466742/
https://www.ncbi.nlm.nih.gov/pubmed/28599669
http://dx.doi.org/10.1186/s13071-017-2229-y
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author Ma, Shuai
Zai, Jinli
Han, Yanhui
Hong, Yang
Zhang, Min
Cao, Xiaodan
Han, Qian
Lu, Ke
Zhao, Zhixin
Lin, Jiaojiao
Fu, Zhiqiang
author_facet Ma, Shuai
Zai, Jinli
Han, Yanhui
Hong, Yang
Zhang, Min
Cao, Xiaodan
Han, Qian
Lu, Ke
Zhao, Zhixin
Lin, Jiaojiao
Fu, Zhiqiang
author_sort Ma, Shuai
collection PubMed
description BACKGROUND: Schistosomiasis remains an important global public health problem, as millions of people are at risk of acquiring infection. An ideal method for sustainable control of schistosomiasis would be to develop an efficient vaccine. Schistosomes can survive in the host vascular system by immune evasion, regulating the host complement cascade. Schistosoma japonicum tetraspanning orphan receptor (SjTOR) is a complement regulator, which is a tegument membrane protein. To date there is no experimental evidence to explain the function of SjTOR. RESULTS: We cloned the first extracellular domain of the SjTOR (SjTOR-ed1) gene and expressed the gene in Escherichia coli. The expression level of SjTOR in different developmental stages of S. japonicum was assessed by quantitative real-time RT-PCR. Western blotting showed that recombinant SjTOR-ed1 (rSjTOR-ed1) could be recognised by schistosome-infected mouse serum. Immunolocalization indicated that the protein was mainly distributed on the tegument of the parasite. Haemolytic assays and ELISA revealed that rSjTOR-ed1 could inhibit complement hemolysis and bind to complement C2. Purified rSjTOR-ed1 emulsified with ISA206 adjuvant could induce a significant reduction of worm burden from 24.51 to 26.51%, and liver egg numbers from 32.92 to 39.62% in two independent trials in mice. CONCLUSIONS: The results of this study indicated that rSjTOR-ed1 could inhibit complement hemolysis and bind to complement C2, and it is a potential vaccine candidate that protects against S. japonicum infection.
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spelling pubmed-54667422017-06-14 Characterization of Schistosoma japonicum tetraspanning orphan receptor and its role in binding to complement C2 and immunoprotection against murine schistosomiasis Ma, Shuai Zai, Jinli Han, Yanhui Hong, Yang Zhang, Min Cao, Xiaodan Han, Qian Lu, Ke Zhao, Zhixin Lin, Jiaojiao Fu, Zhiqiang Parasit Vectors Research BACKGROUND: Schistosomiasis remains an important global public health problem, as millions of people are at risk of acquiring infection. An ideal method for sustainable control of schistosomiasis would be to develop an efficient vaccine. Schistosomes can survive in the host vascular system by immune evasion, regulating the host complement cascade. Schistosoma japonicum tetraspanning orphan receptor (SjTOR) is a complement regulator, which is a tegument membrane protein. To date there is no experimental evidence to explain the function of SjTOR. RESULTS: We cloned the first extracellular domain of the SjTOR (SjTOR-ed1) gene and expressed the gene in Escherichia coli. The expression level of SjTOR in different developmental stages of S. japonicum was assessed by quantitative real-time RT-PCR. Western blotting showed that recombinant SjTOR-ed1 (rSjTOR-ed1) could be recognised by schistosome-infected mouse serum. Immunolocalization indicated that the protein was mainly distributed on the tegument of the parasite. Haemolytic assays and ELISA revealed that rSjTOR-ed1 could inhibit complement hemolysis and bind to complement C2. Purified rSjTOR-ed1 emulsified with ISA206 adjuvant could induce a significant reduction of worm burden from 24.51 to 26.51%, and liver egg numbers from 32.92 to 39.62% in two independent trials in mice. CONCLUSIONS: The results of this study indicated that rSjTOR-ed1 could inhibit complement hemolysis and bind to complement C2, and it is a potential vaccine candidate that protects against S. japonicum infection. BioMed Central 2017-06-09 /pmc/articles/PMC5466742/ /pubmed/28599669 http://dx.doi.org/10.1186/s13071-017-2229-y Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ma, Shuai
Zai, Jinli
Han, Yanhui
Hong, Yang
Zhang, Min
Cao, Xiaodan
Han, Qian
Lu, Ke
Zhao, Zhixin
Lin, Jiaojiao
Fu, Zhiqiang
Characterization of Schistosoma japonicum tetraspanning orphan receptor and its role in binding to complement C2 and immunoprotection against murine schistosomiasis
title Characterization of Schistosoma japonicum tetraspanning orphan receptor and its role in binding to complement C2 and immunoprotection against murine schistosomiasis
title_full Characterization of Schistosoma japonicum tetraspanning orphan receptor and its role in binding to complement C2 and immunoprotection against murine schistosomiasis
title_fullStr Characterization of Schistosoma japonicum tetraspanning orphan receptor and its role in binding to complement C2 and immunoprotection against murine schistosomiasis
title_full_unstemmed Characterization of Schistosoma japonicum tetraspanning orphan receptor and its role in binding to complement C2 and immunoprotection against murine schistosomiasis
title_short Characterization of Schistosoma japonicum tetraspanning orphan receptor and its role in binding to complement C2 and immunoprotection against murine schistosomiasis
title_sort characterization of schistosoma japonicum tetraspanning orphan receptor and its role in binding to complement c2 and immunoprotection against murine schistosomiasis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466742/
https://www.ncbi.nlm.nih.gov/pubmed/28599669
http://dx.doi.org/10.1186/s13071-017-2229-y
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