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DNA vaccination protects mice against Zika virus-induced damage to the testes
Zika virus (ZIKV) is an emerging pathogen causally associated with serious sequelae in fetuses, inducing fetal microcephaly and other neurodevelopment defects. ZIKV is primarily transmitted by mosquitoes, but can persist in human semen and sperm, and sexual transmission has been documented. Moreover...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5467228/ https://www.ncbi.nlm.nih.gov/pubmed/28589934 http://dx.doi.org/10.1038/ncomms15743 |
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author | Griffin, Bryan D. Muthumani, Kar Warner, Bryce M. Majer, Anna Hagan, Mable Audet, Jonathan Stein, Derek R. Ranadheera, Charlene Racine, Trina De La Vega, Marc-Antoine Piret, Jocelyne Kucas, Stephanie Tran, Kaylie N. Frost, Kathy L. De Graff, Christine Soule, Geoff Scharikow, Leanne Scott, Jennifer McTavish, Gordon Smid, Valerie Park, Young K. Maslow, Joel N. Sardesai, Niranjan Y. Kim, J. Joseph Yao, Xiao-jian Bello, Alexander Lindsay, Robbin Boivin, Guy Booth, Stephanie A. Kobasa, Darwyn Embury-Hyatt, Carissa Safronetz, David Weiner, David B. Kobinger, Gary P. |
author_facet | Griffin, Bryan D. Muthumani, Kar Warner, Bryce M. Majer, Anna Hagan, Mable Audet, Jonathan Stein, Derek R. Ranadheera, Charlene Racine, Trina De La Vega, Marc-Antoine Piret, Jocelyne Kucas, Stephanie Tran, Kaylie N. Frost, Kathy L. De Graff, Christine Soule, Geoff Scharikow, Leanne Scott, Jennifer McTavish, Gordon Smid, Valerie Park, Young K. Maslow, Joel N. Sardesai, Niranjan Y. Kim, J. Joseph Yao, Xiao-jian Bello, Alexander Lindsay, Robbin Boivin, Guy Booth, Stephanie A. Kobasa, Darwyn Embury-Hyatt, Carissa Safronetz, David Weiner, David B. Kobinger, Gary P. |
author_sort | Griffin, Bryan D. |
collection | PubMed |
description | Zika virus (ZIKV) is an emerging pathogen causally associated with serious sequelae in fetuses, inducing fetal microcephaly and other neurodevelopment defects. ZIKV is primarily transmitted by mosquitoes, but can persist in human semen and sperm, and sexual transmission has been documented. Moreover, exposure of type-I interferon knockout mice to ZIKV results in severe damage to the testes, epididymis and sperm. Candidate ZIKV vaccines have shown protective efficacy in preclinical studies carried out in animal models, and several vaccines have entered clinical trials. Here, we report that administration of a synthetic DNA vaccine encoding ZIKV pre-membrane and envelope (prME) completely protects mice against ZIKV-associated damage to the testes and sperm and prevents viral persistence in the testes following challenge with a contemporary strain of ZIKV. These data suggest that DNA vaccination merits further investigation as a potential means to reduce ZIKV persistence in the male reproductive tract. |
format | Online Article Text |
id | pubmed-5467228 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-54672282017-06-19 DNA vaccination protects mice against Zika virus-induced damage to the testes Griffin, Bryan D. Muthumani, Kar Warner, Bryce M. Majer, Anna Hagan, Mable Audet, Jonathan Stein, Derek R. Ranadheera, Charlene Racine, Trina De La Vega, Marc-Antoine Piret, Jocelyne Kucas, Stephanie Tran, Kaylie N. Frost, Kathy L. De Graff, Christine Soule, Geoff Scharikow, Leanne Scott, Jennifer McTavish, Gordon Smid, Valerie Park, Young K. Maslow, Joel N. Sardesai, Niranjan Y. Kim, J. Joseph Yao, Xiao-jian Bello, Alexander Lindsay, Robbin Boivin, Guy Booth, Stephanie A. Kobasa, Darwyn Embury-Hyatt, Carissa Safronetz, David Weiner, David B. Kobinger, Gary P. Nat Commun Article Zika virus (ZIKV) is an emerging pathogen causally associated with serious sequelae in fetuses, inducing fetal microcephaly and other neurodevelopment defects. ZIKV is primarily transmitted by mosquitoes, but can persist in human semen and sperm, and sexual transmission has been documented. Moreover, exposure of type-I interferon knockout mice to ZIKV results in severe damage to the testes, epididymis and sperm. Candidate ZIKV vaccines have shown protective efficacy in preclinical studies carried out in animal models, and several vaccines have entered clinical trials. Here, we report that administration of a synthetic DNA vaccine encoding ZIKV pre-membrane and envelope (prME) completely protects mice against ZIKV-associated damage to the testes and sperm and prevents viral persistence in the testes following challenge with a contemporary strain of ZIKV. These data suggest that DNA vaccination merits further investigation as a potential means to reduce ZIKV persistence in the male reproductive tract. Nature Publishing Group 2017-06-07 /pmc/articles/PMC5467228/ /pubmed/28589934 http://dx.doi.org/10.1038/ncomms15743 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Griffin, Bryan D. Muthumani, Kar Warner, Bryce M. Majer, Anna Hagan, Mable Audet, Jonathan Stein, Derek R. Ranadheera, Charlene Racine, Trina De La Vega, Marc-Antoine Piret, Jocelyne Kucas, Stephanie Tran, Kaylie N. Frost, Kathy L. De Graff, Christine Soule, Geoff Scharikow, Leanne Scott, Jennifer McTavish, Gordon Smid, Valerie Park, Young K. Maslow, Joel N. Sardesai, Niranjan Y. Kim, J. Joseph Yao, Xiao-jian Bello, Alexander Lindsay, Robbin Boivin, Guy Booth, Stephanie A. Kobasa, Darwyn Embury-Hyatt, Carissa Safronetz, David Weiner, David B. Kobinger, Gary P. DNA vaccination protects mice against Zika virus-induced damage to the testes |
title | DNA vaccination protects mice against Zika virus-induced damage to the testes |
title_full | DNA vaccination protects mice against Zika virus-induced damage to the testes |
title_fullStr | DNA vaccination protects mice against Zika virus-induced damage to the testes |
title_full_unstemmed | DNA vaccination protects mice against Zika virus-induced damage to the testes |
title_short | DNA vaccination protects mice against Zika virus-induced damage to the testes |
title_sort | dna vaccination protects mice against zika virus-induced damage to the testes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5467228/ https://www.ncbi.nlm.nih.gov/pubmed/28589934 http://dx.doi.org/10.1038/ncomms15743 |
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