Cargando…
Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen
The circadian clock is reported to play a role in the ovaries in a variety of vertebrate species, including the domestic hen. However, the ovary is an organ that changes daily, and the laying hen maintains a strict follicular hierarchy. The aim of this study was to examine the spatial-temporal expre...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5467841/ https://www.ncbi.nlm.nih.gov/pubmed/28604799 http://dx.doi.org/10.1371/journal.pone.0179019 |
_version_ | 1783243327100944384 |
---|---|
author | Zhang, Zhichao Lai, Shuang Wang, Yagang Li, Liang Yin, Huadong Wang, Yan Zhao, Xiaoling Li, Diyan Yang, Mingyao Zhu, Qing |
author_facet | Zhang, Zhichao Lai, Shuang Wang, Yagang Li, Liang Yin, Huadong Wang, Yan Zhao, Xiaoling Li, Diyan Yang, Mingyao Zhu, Qing |
author_sort | Zhang, Zhichao |
collection | PubMed |
description | The circadian clock is reported to play a role in the ovaries in a variety of vertebrate species, including the domestic hen. However, the ovary is an organ that changes daily, and the laying hen maintains a strict follicular hierarchy. The aim of this study was to examine the spatial-temporal expression of several known canonical clock genes in the granulosa and theca layers of six hierarchy follicles. We demonstrated that the granulosa cells (GCs) of the F1-F3 follicles harbored intrinsic oscillatory mechanisms in vivo. In addition, cultured granulosa cells (GCs) from F1 follicles exposed to luteinizing hormone (LH) synchronization displayed Per2 mRNA oscillations, whereas, the less mature GCs (F5 plus F6) displayed no circadian change in Per2 mRNA levels. Cultures containing follicle-stimulating hormone (FSH) combined with LH expressed levels of Per2 mRNA that were 2.5-fold higher than those in cultures with LH or FSH alone. These results show that there is spatial specificity in the localization of clock cells in hen preovulatory follicles. In addition, our results support the hypothesis that gonadotropins provide a cue for the development of the functional cellular clock in immature GCs. |
format | Online Article Text |
id | pubmed-5467841 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54678412017-06-22 Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen Zhang, Zhichao Lai, Shuang Wang, Yagang Li, Liang Yin, Huadong Wang, Yan Zhao, Xiaoling Li, Diyan Yang, Mingyao Zhu, Qing PLoS One Research Article The circadian clock is reported to play a role in the ovaries in a variety of vertebrate species, including the domestic hen. However, the ovary is an organ that changes daily, and the laying hen maintains a strict follicular hierarchy. The aim of this study was to examine the spatial-temporal expression of several known canonical clock genes in the granulosa and theca layers of six hierarchy follicles. We demonstrated that the granulosa cells (GCs) of the F1-F3 follicles harbored intrinsic oscillatory mechanisms in vivo. In addition, cultured granulosa cells (GCs) from F1 follicles exposed to luteinizing hormone (LH) synchronization displayed Per2 mRNA oscillations, whereas, the less mature GCs (F5 plus F6) displayed no circadian change in Per2 mRNA levels. Cultures containing follicle-stimulating hormone (FSH) combined with LH expressed levels of Per2 mRNA that were 2.5-fold higher than those in cultures with LH or FSH alone. These results show that there is spatial specificity in the localization of clock cells in hen preovulatory follicles. In addition, our results support the hypothesis that gonadotropins provide a cue for the development of the functional cellular clock in immature GCs. Public Library of Science 2017-06-12 /pmc/articles/PMC5467841/ /pubmed/28604799 http://dx.doi.org/10.1371/journal.pone.0179019 Text en © 2017 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhang, Zhichao Lai, Shuang Wang, Yagang Li, Liang Yin, Huadong Wang, Yan Zhao, Xiaoling Li, Diyan Yang, Mingyao Zhu, Qing Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen |
title | Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen |
title_full | Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen |
title_fullStr | Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen |
title_full_unstemmed | Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen |
title_short | Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen |
title_sort | rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5467841/ https://www.ncbi.nlm.nih.gov/pubmed/28604799 http://dx.doi.org/10.1371/journal.pone.0179019 |
work_keys_str_mv | AT zhangzhichao rhythmicexpressionofcircadianclockgenesinthepreovulatoryovarianfolliclesofthelayinghen AT laishuang rhythmicexpressionofcircadianclockgenesinthepreovulatoryovarianfolliclesofthelayinghen AT wangyagang rhythmicexpressionofcircadianclockgenesinthepreovulatoryovarianfolliclesofthelayinghen AT liliang rhythmicexpressionofcircadianclockgenesinthepreovulatoryovarianfolliclesofthelayinghen AT yinhuadong rhythmicexpressionofcircadianclockgenesinthepreovulatoryovarianfolliclesofthelayinghen AT wangyan rhythmicexpressionofcircadianclockgenesinthepreovulatoryovarianfolliclesofthelayinghen AT zhaoxiaoling rhythmicexpressionofcircadianclockgenesinthepreovulatoryovarianfolliclesofthelayinghen AT lidiyan rhythmicexpressionofcircadianclockgenesinthepreovulatoryovarianfolliclesofthelayinghen AT yangmingyao rhythmicexpressionofcircadianclockgenesinthepreovulatoryovarianfolliclesofthelayinghen AT zhuqing rhythmicexpressionofcircadianclockgenesinthepreovulatoryovarianfolliclesofthelayinghen |