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Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius

The bed bug Cimex lectularius is a blood-feeding re-emerging annoyance pest insect that has the ability to transmit Trypanosoma cruzi under experimental laboratory conditions. Aquaporins (AQPs) are water channel proteins that are essential in biological organisms. C. lectularius are constantly expos...

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Autores principales: Tsujimoto, Hitoshi, Sakamoto, Joyce M., Rasgon, Jason L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5468273/
https://www.ncbi.nlm.nih.gov/pubmed/28607409
http://dx.doi.org/10.1038/s41598-017-03157-2
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author Tsujimoto, Hitoshi
Sakamoto, Joyce M.
Rasgon, Jason L.
author_facet Tsujimoto, Hitoshi
Sakamoto, Joyce M.
Rasgon, Jason L.
author_sort Tsujimoto, Hitoshi
collection PubMed
description The bed bug Cimex lectularius is a blood-feeding re-emerging annoyance pest insect that has the ability to transmit Trypanosoma cruzi under experimental laboratory conditions. Aquaporins (AQPs) are water channel proteins that are essential in biological organisms. C. lectularius are constantly exposed to water-related stress, suggesting that AQPs may offer novel control avenues. We identified and cloned four AQPs from C. lectularius, assessed tissue and lifestage-specific expression, and characterized biochemical functions in vitro and in vivo. We identified an efficient water-specific AQP (ClAQP1), two aquaglyceroporins (ClGlp1 and ClGlp2) and a homolog of Drosophila melanogaster big brain (ClBib). ClGlp1 was only functional when co-expressed with the water-specific AQP. Simultaneous RNAi gene silencing of ClAQP1 and ClGlp1 significantly reduced water and urea excretion post blood feeding. The Bib homologue was enriched in embryos, exclusively expressed in ovaries, and when silenced, dramatically increased bug fecundity. Our data demonstrate that AQPs have critical roles in excretion, water homeostasis and reproduction in C. lectularius, and could be potential targets for control in this notorious pest.
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spelling pubmed-54682732017-06-14 Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius Tsujimoto, Hitoshi Sakamoto, Joyce M. Rasgon, Jason L. Sci Rep Article The bed bug Cimex lectularius is a blood-feeding re-emerging annoyance pest insect that has the ability to transmit Trypanosoma cruzi under experimental laboratory conditions. Aquaporins (AQPs) are water channel proteins that are essential in biological organisms. C. lectularius are constantly exposed to water-related stress, suggesting that AQPs may offer novel control avenues. We identified and cloned four AQPs from C. lectularius, assessed tissue and lifestage-specific expression, and characterized biochemical functions in vitro and in vivo. We identified an efficient water-specific AQP (ClAQP1), two aquaglyceroporins (ClGlp1 and ClGlp2) and a homolog of Drosophila melanogaster big brain (ClBib). ClGlp1 was only functional when co-expressed with the water-specific AQP. Simultaneous RNAi gene silencing of ClAQP1 and ClGlp1 significantly reduced water and urea excretion post blood feeding. The Bib homologue was enriched in embryos, exclusively expressed in ovaries, and when silenced, dramatically increased bug fecundity. Our data demonstrate that AQPs have critical roles in excretion, water homeostasis and reproduction in C. lectularius, and could be potential targets for control in this notorious pest. Nature Publishing Group UK 2017-06-12 /pmc/articles/PMC5468273/ /pubmed/28607409 http://dx.doi.org/10.1038/s41598-017-03157-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tsujimoto, Hitoshi
Sakamoto, Joyce M.
Rasgon, Jason L.
Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
title Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
title_full Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
title_fullStr Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
title_full_unstemmed Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
title_short Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
title_sort functional characterization of aquaporin-like genes in the human bed bug cimex lectularius
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5468273/
https://www.ncbi.nlm.nih.gov/pubmed/28607409
http://dx.doi.org/10.1038/s41598-017-03157-2
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