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Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis

Leptospirosis is underdiagnosed due to low sensitivity, need of specialised equipment, and expensive reagents for serological and molecular diagnosis respectively. Considering the sensitivity, rapidity, inexpensive reagents and collection of clinical samples, the monoclonal antibody based antigen de...

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Autores principales: Kanagavel, Murugesan, Shanmughapriya, Santhanam, Aishwarya, Kayanam Vijaya Lalitha, Ponmurugan, Karuppiah, Murugan, Kasi, Al-Dhabi, Naif Abdullah, Natarajaseenivasan, Kalimuthusamy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5468321/
https://www.ncbi.nlm.nih.gov/pubmed/28607384
http://dx.doi.org/10.1038/s41598-017-03658-0
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author Kanagavel, Murugesan
Shanmughapriya, Santhanam
Aishwarya, Kayanam Vijaya Lalitha
Ponmurugan, Karuppiah
Murugan, Kasi
Al-Dhabi, Naif Abdullah
Natarajaseenivasan, Kalimuthusamy
author_facet Kanagavel, Murugesan
Shanmughapriya, Santhanam
Aishwarya, Kayanam Vijaya Lalitha
Ponmurugan, Karuppiah
Murugan, Kasi
Al-Dhabi, Naif Abdullah
Natarajaseenivasan, Kalimuthusamy
author_sort Kanagavel, Murugesan
collection PubMed
description Leptospirosis is underdiagnosed due to low sensitivity, need of specialised equipment, and expensive reagents for serological and molecular diagnosis respectively. Considering the sensitivity, rapidity, inexpensive reagents and collection of clinical samples, the monoclonal antibody based antigen detection method from urine samples has been developed and evaluated. LigA (LK90) based B-cell specific epitopes were predicted and synthesised as peptides for the production of monoclonal antibody. LK90(543): SNAQKNQGNA (amino acids: 543 to 552), and LK90(1110): DHHTQSSYTP (amino acids: 1110 to 1119) with VaxiJen score of 1.3719 and 1.2215, respectively were used. Thirty two and 28 urine samples from confirmed and seronegative healthy human subjects, respectively were included for the evaluation of MAb-based dot blot ELISA. The specificity of the evaluated MAbs, P1B1 and P4W2 were found to be in the range of ~93–96%. Moreover, the MAbs did not show cross-reactivity with other bacterial antigens as confirmed by IgG ELISA, further validating its specificity for leptospiral antigens. These findings suggest that the developed MAb based dot blot ELISA is a simple, rapid performed in less than 8 h, inexpensive with a ICER of $8.7/QALY, and affordable in developing countries and area where laboratory facilities are limited.
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spelling pubmed-54683212017-06-14 Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis Kanagavel, Murugesan Shanmughapriya, Santhanam Aishwarya, Kayanam Vijaya Lalitha Ponmurugan, Karuppiah Murugan, Kasi Al-Dhabi, Naif Abdullah Natarajaseenivasan, Kalimuthusamy Sci Rep Article Leptospirosis is underdiagnosed due to low sensitivity, need of specialised equipment, and expensive reagents for serological and molecular diagnosis respectively. Considering the sensitivity, rapidity, inexpensive reagents and collection of clinical samples, the monoclonal antibody based antigen detection method from urine samples has been developed and evaluated. LigA (LK90) based B-cell specific epitopes were predicted and synthesised as peptides for the production of monoclonal antibody. LK90(543): SNAQKNQGNA (amino acids: 543 to 552), and LK90(1110): DHHTQSSYTP (amino acids: 1110 to 1119) with VaxiJen score of 1.3719 and 1.2215, respectively were used. Thirty two and 28 urine samples from confirmed and seronegative healthy human subjects, respectively were included for the evaluation of MAb-based dot blot ELISA. The specificity of the evaluated MAbs, P1B1 and P4W2 were found to be in the range of ~93–96%. Moreover, the MAbs did not show cross-reactivity with other bacterial antigens as confirmed by IgG ELISA, further validating its specificity for leptospiral antigens. These findings suggest that the developed MAb based dot blot ELISA is a simple, rapid performed in less than 8 h, inexpensive with a ICER of $8.7/QALY, and affordable in developing countries and area where laboratory facilities are limited. Nature Publishing Group UK 2017-06-12 /pmc/articles/PMC5468321/ /pubmed/28607384 http://dx.doi.org/10.1038/s41598-017-03658-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kanagavel, Murugesan
Shanmughapriya, Santhanam
Aishwarya, Kayanam Vijaya Lalitha
Ponmurugan, Karuppiah
Murugan, Kasi
Al-Dhabi, Naif Abdullah
Natarajaseenivasan, Kalimuthusamy
Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
title Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
title_full Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
title_fullStr Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
title_full_unstemmed Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
title_short Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
title_sort peptide specific monoclonal antibodies of leptospiral liga for acute diagnosis of leptospirosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5468321/
https://www.ncbi.nlm.nih.gov/pubmed/28607384
http://dx.doi.org/10.1038/s41598-017-03658-0
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