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Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
Leptospirosis is underdiagnosed due to low sensitivity, need of specialised equipment, and expensive reagents for serological and molecular diagnosis respectively. Considering the sensitivity, rapidity, inexpensive reagents and collection of clinical samples, the monoclonal antibody based antigen de...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5468321/ https://www.ncbi.nlm.nih.gov/pubmed/28607384 http://dx.doi.org/10.1038/s41598-017-03658-0 |
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author | Kanagavel, Murugesan Shanmughapriya, Santhanam Aishwarya, Kayanam Vijaya Lalitha Ponmurugan, Karuppiah Murugan, Kasi Al-Dhabi, Naif Abdullah Natarajaseenivasan, Kalimuthusamy |
author_facet | Kanagavel, Murugesan Shanmughapriya, Santhanam Aishwarya, Kayanam Vijaya Lalitha Ponmurugan, Karuppiah Murugan, Kasi Al-Dhabi, Naif Abdullah Natarajaseenivasan, Kalimuthusamy |
author_sort | Kanagavel, Murugesan |
collection | PubMed |
description | Leptospirosis is underdiagnosed due to low sensitivity, need of specialised equipment, and expensive reagents for serological and molecular diagnosis respectively. Considering the sensitivity, rapidity, inexpensive reagents and collection of clinical samples, the monoclonal antibody based antigen detection method from urine samples has been developed and evaluated. LigA (LK90) based B-cell specific epitopes were predicted and synthesised as peptides for the production of monoclonal antibody. LK90(543): SNAQKNQGNA (amino acids: 543 to 552), and LK90(1110): DHHTQSSYTP (amino acids: 1110 to 1119) with VaxiJen score of 1.3719 and 1.2215, respectively were used. Thirty two and 28 urine samples from confirmed and seronegative healthy human subjects, respectively were included for the evaluation of MAb-based dot blot ELISA. The specificity of the evaluated MAbs, P1B1 and P4W2 were found to be in the range of ~93–96%. Moreover, the MAbs did not show cross-reactivity with other bacterial antigens as confirmed by IgG ELISA, further validating its specificity for leptospiral antigens. These findings suggest that the developed MAb based dot blot ELISA is a simple, rapid performed in less than 8 h, inexpensive with a ICER of $8.7/QALY, and affordable in developing countries and area where laboratory facilities are limited. |
format | Online Article Text |
id | pubmed-5468321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54683212017-06-14 Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis Kanagavel, Murugesan Shanmughapriya, Santhanam Aishwarya, Kayanam Vijaya Lalitha Ponmurugan, Karuppiah Murugan, Kasi Al-Dhabi, Naif Abdullah Natarajaseenivasan, Kalimuthusamy Sci Rep Article Leptospirosis is underdiagnosed due to low sensitivity, need of specialised equipment, and expensive reagents for serological and molecular diagnosis respectively. Considering the sensitivity, rapidity, inexpensive reagents and collection of clinical samples, the monoclonal antibody based antigen detection method from urine samples has been developed and evaluated. LigA (LK90) based B-cell specific epitopes were predicted and synthesised as peptides for the production of monoclonal antibody. LK90(543): SNAQKNQGNA (amino acids: 543 to 552), and LK90(1110): DHHTQSSYTP (amino acids: 1110 to 1119) with VaxiJen score of 1.3719 and 1.2215, respectively were used. Thirty two and 28 urine samples from confirmed and seronegative healthy human subjects, respectively were included for the evaluation of MAb-based dot blot ELISA. The specificity of the evaluated MAbs, P1B1 and P4W2 were found to be in the range of ~93–96%. Moreover, the MAbs did not show cross-reactivity with other bacterial antigens as confirmed by IgG ELISA, further validating its specificity for leptospiral antigens. These findings suggest that the developed MAb based dot blot ELISA is a simple, rapid performed in less than 8 h, inexpensive with a ICER of $8.7/QALY, and affordable in developing countries and area where laboratory facilities are limited. Nature Publishing Group UK 2017-06-12 /pmc/articles/PMC5468321/ /pubmed/28607384 http://dx.doi.org/10.1038/s41598-017-03658-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kanagavel, Murugesan Shanmughapriya, Santhanam Aishwarya, Kayanam Vijaya Lalitha Ponmurugan, Karuppiah Murugan, Kasi Al-Dhabi, Naif Abdullah Natarajaseenivasan, Kalimuthusamy Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis |
title | Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis |
title_full | Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis |
title_fullStr | Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis |
title_full_unstemmed | Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis |
title_short | Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis |
title_sort | peptide specific monoclonal antibodies of leptospiral liga for acute diagnosis of leptospirosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5468321/ https://www.ncbi.nlm.nih.gov/pubmed/28607384 http://dx.doi.org/10.1038/s41598-017-03658-0 |
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