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Erzhi Pill® Repairs Experimental Liver Injury via TSC/mTOR Signaling Pathway Inhibiting Excessive Apoptosis

The present study aimed to investigate the mechanism of hepatoprotective effect of Erzhi Pill (EZP) on the liver injury via observing TSC/mTOR signaling pathway activation. The experimental liver injury was induced by 2-acetylaminofluorene (2-AAF) treatment combined with partial hepatectomy (PH). EZ...

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Autores principales: Zhou, Bu-gao, Zhao, Hai-mei, Lu, Xiu-yun, Wang, Xin, Zou, Yong, Xu, Rong, Yue, Hai-yang, Liu, Yi, Zuo, Zheng-yun, Liu, Duan-yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5468563/
https://www.ncbi.nlm.nih.gov/pubmed/28638431
http://dx.doi.org/10.1155/2017/5653643
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author Zhou, Bu-gao
Zhao, Hai-mei
Lu, Xiu-yun
Wang, Xin
Zou, Yong
Xu, Rong
Yue, Hai-yang
Liu, Yi
Zuo, Zheng-yun
Liu, Duan-yong
author_facet Zhou, Bu-gao
Zhao, Hai-mei
Lu, Xiu-yun
Wang, Xin
Zou, Yong
Xu, Rong
Yue, Hai-yang
Liu, Yi
Zuo, Zheng-yun
Liu, Duan-yong
author_sort Zhou, Bu-gao
collection PubMed
description The present study aimed to investigate the mechanism of hepatoprotective effect of Erzhi Pill (EZP) on the liver injury via observing TSC/mTOR signaling pathway activation. The experimental liver injury was induced by 2-acetylaminofluorene (2-AAF) treatment combined with partial hepatectomy (PH). EZP treated 2-AAF/PH-induced liver injury by the therapeutic and prophylactic administration. After the administration of EZP, the activities of aspartic transaminase (AST), alanine aminotransferase (ALT), alkaline phosphatase (AKP), and gamma-glutamyl transpeptidase (γ-GT) were decreased, followed by the decreased levels of hepatocyte apoptosis and caspase-3 expression. However, the secretion of albumin, liver weight, and index of liver weight were elevated. Microscopic examination showed that EZP restored pathological liver injury. Meanwhile, Rheb and mammalian target of rapamycin (mTOR) activation were suppressed, and tuberous sclerosis complex (TSC) expression was elevated in liver tissues induced by 2-AAF/PHx and accompanied with lower-expression of Bax, Notch1, p70S6K, and 4E-EIF and upregulated levels of Bcl-2 and Cyclin D. Hepatoprotective effect of EZP was possibly realized via inhibiting TSC/mTOR signaling pathway to suppress excessive apoptosis of hepatocyte.
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spelling pubmed-54685632017-06-21 Erzhi Pill® Repairs Experimental Liver Injury via TSC/mTOR Signaling Pathway Inhibiting Excessive Apoptosis Zhou, Bu-gao Zhao, Hai-mei Lu, Xiu-yun Wang, Xin Zou, Yong Xu, Rong Yue, Hai-yang Liu, Yi Zuo, Zheng-yun Liu, Duan-yong Evid Based Complement Alternat Med Research Article The present study aimed to investigate the mechanism of hepatoprotective effect of Erzhi Pill (EZP) on the liver injury via observing TSC/mTOR signaling pathway activation. The experimental liver injury was induced by 2-acetylaminofluorene (2-AAF) treatment combined with partial hepatectomy (PH). EZP treated 2-AAF/PH-induced liver injury by the therapeutic and prophylactic administration. After the administration of EZP, the activities of aspartic transaminase (AST), alanine aminotransferase (ALT), alkaline phosphatase (AKP), and gamma-glutamyl transpeptidase (γ-GT) were decreased, followed by the decreased levels of hepatocyte apoptosis and caspase-3 expression. However, the secretion of albumin, liver weight, and index of liver weight were elevated. Microscopic examination showed that EZP restored pathological liver injury. Meanwhile, Rheb and mammalian target of rapamycin (mTOR) activation were suppressed, and tuberous sclerosis complex (TSC) expression was elevated in liver tissues induced by 2-AAF/PHx and accompanied with lower-expression of Bax, Notch1, p70S6K, and 4E-EIF and upregulated levels of Bcl-2 and Cyclin D. Hepatoprotective effect of EZP was possibly realized via inhibiting TSC/mTOR signaling pathway to suppress excessive apoptosis of hepatocyte. Hindawi 2017 2017-05-30 /pmc/articles/PMC5468563/ /pubmed/28638431 http://dx.doi.org/10.1155/2017/5653643 Text en Copyright © 2017 Bu-gao Zhou et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhou, Bu-gao
Zhao, Hai-mei
Lu, Xiu-yun
Wang, Xin
Zou, Yong
Xu, Rong
Yue, Hai-yang
Liu, Yi
Zuo, Zheng-yun
Liu, Duan-yong
Erzhi Pill® Repairs Experimental Liver Injury via TSC/mTOR Signaling Pathway Inhibiting Excessive Apoptosis
title Erzhi Pill® Repairs Experimental Liver Injury via TSC/mTOR Signaling Pathway Inhibiting Excessive Apoptosis
title_full Erzhi Pill® Repairs Experimental Liver Injury via TSC/mTOR Signaling Pathway Inhibiting Excessive Apoptosis
title_fullStr Erzhi Pill® Repairs Experimental Liver Injury via TSC/mTOR Signaling Pathway Inhibiting Excessive Apoptosis
title_full_unstemmed Erzhi Pill® Repairs Experimental Liver Injury via TSC/mTOR Signaling Pathway Inhibiting Excessive Apoptosis
title_short Erzhi Pill® Repairs Experimental Liver Injury via TSC/mTOR Signaling Pathway Inhibiting Excessive Apoptosis
title_sort erzhi pill® repairs experimental liver injury via tsc/mtor signaling pathway inhibiting excessive apoptosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5468563/
https://www.ncbi.nlm.nih.gov/pubmed/28638431
http://dx.doi.org/10.1155/2017/5653643
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