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Prevalence of pks-positive Escherichia coli in Japanese patients with or without colorectal cancer
BACKGROUND: Recent studies show that some Escherichia coli strains possessing a gene cluster named the pks island might have a causative role in the development of human colorectal cancer (CRC). In several reports from Europe, they are found more prevalently in colon tissue specimens derived from CR...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5468999/ https://www.ncbi.nlm.nih.gov/pubmed/28616082 http://dx.doi.org/10.1186/s13099-017-0185-x |
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author | Shimpoh, Takayuki Hirata, Yoshihiro Ihara, Sozaburo Suzuki, Nobumi Kinoshita, Hiroto Hayakawa, Yoku Ota, Yumiko Narita, Akiko Yoshida, Shuntaro Yamada, Atsuo Koike, Kazuhiko |
author_facet | Shimpoh, Takayuki Hirata, Yoshihiro Ihara, Sozaburo Suzuki, Nobumi Kinoshita, Hiroto Hayakawa, Yoku Ota, Yumiko Narita, Akiko Yoshida, Shuntaro Yamada, Atsuo Koike, Kazuhiko |
author_sort | Shimpoh, Takayuki |
collection | PubMed |
description | BACKGROUND: Recent studies show that some Escherichia coli strains possessing a gene cluster named the pks island might have a causative role in the development of human colorectal cancer (CRC). In several reports from Europe, they are found more prevalently in colon tissue specimens derived from CRC patients compared to those from controls. In this study we sought to clarify the difference in pks prevalence between CRC patients and non-CRC controls in the Japanese population, by using non-invasive sample collection technique during colonoscopy. METHODS: Colonic lavage samples were collected during diagnostic colonoscopy, and bacterial DNA within each sample was extracted. Fecal DNA samples were then examined for pks island genes using conventional qualitative PCR and real-time quantitative PCR. In some patients biopsy samples were also collected in the same session of colonoscopy, and the correlation between the pks status of the colonic lavage sample and the biopsy sample of the same patients was evaluated. RESULTS: Twelve out of thirteen patients (92%) showed the same pks status by colonic lavage sample and biopsy sample, suggesting the usefulness of colonic lavage samples as a surrogate for biopsy samples. A total of 98 colonic lavage samples were collected, which included 35 from CRC patients, 37 from adenoma patients, and 26 from controls. The pks-positive bacterial DNA was detected in 43, 51, and 46% of colonic lavage samples from CRC, adenoma, and control patients, respectively, and there was no significant difference among diseases. Real-time quantitative PCR showed no significant difference in the relative concentrations of pks-positive bacterial DNA among diseases. Age, gender, location of CRC, CRC staging, or k-ras gene status was not associated with pks prevalence. CONCLUSIONS: Although the method of collecting fecal DNA from colonic lavage samples was safe and technically feasible, factors other than pks-positive bacteria appear to play more important roles in CRC development in this cohort. |
format | Online Article Text |
id | pubmed-5468999 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54689992017-06-14 Prevalence of pks-positive Escherichia coli in Japanese patients with or without colorectal cancer Shimpoh, Takayuki Hirata, Yoshihiro Ihara, Sozaburo Suzuki, Nobumi Kinoshita, Hiroto Hayakawa, Yoku Ota, Yumiko Narita, Akiko Yoshida, Shuntaro Yamada, Atsuo Koike, Kazuhiko Gut Pathog Research BACKGROUND: Recent studies show that some Escherichia coli strains possessing a gene cluster named the pks island might have a causative role in the development of human colorectal cancer (CRC). In several reports from Europe, they are found more prevalently in colon tissue specimens derived from CRC patients compared to those from controls. In this study we sought to clarify the difference in pks prevalence between CRC patients and non-CRC controls in the Japanese population, by using non-invasive sample collection technique during colonoscopy. METHODS: Colonic lavage samples were collected during diagnostic colonoscopy, and bacterial DNA within each sample was extracted. Fecal DNA samples were then examined for pks island genes using conventional qualitative PCR and real-time quantitative PCR. In some patients biopsy samples were also collected in the same session of colonoscopy, and the correlation between the pks status of the colonic lavage sample and the biopsy sample of the same patients was evaluated. RESULTS: Twelve out of thirteen patients (92%) showed the same pks status by colonic lavage sample and biopsy sample, suggesting the usefulness of colonic lavage samples as a surrogate for biopsy samples. A total of 98 colonic lavage samples were collected, which included 35 from CRC patients, 37 from adenoma patients, and 26 from controls. The pks-positive bacterial DNA was detected in 43, 51, and 46% of colonic lavage samples from CRC, adenoma, and control patients, respectively, and there was no significant difference among diseases. Real-time quantitative PCR showed no significant difference in the relative concentrations of pks-positive bacterial DNA among diseases. Age, gender, location of CRC, CRC staging, or k-ras gene status was not associated with pks prevalence. CONCLUSIONS: Although the method of collecting fecal DNA from colonic lavage samples was safe and technically feasible, factors other than pks-positive bacteria appear to play more important roles in CRC development in this cohort. BioMed Central 2017-06-12 /pmc/articles/PMC5468999/ /pubmed/28616082 http://dx.doi.org/10.1186/s13099-017-0185-x Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Shimpoh, Takayuki Hirata, Yoshihiro Ihara, Sozaburo Suzuki, Nobumi Kinoshita, Hiroto Hayakawa, Yoku Ota, Yumiko Narita, Akiko Yoshida, Shuntaro Yamada, Atsuo Koike, Kazuhiko Prevalence of pks-positive Escherichia coli in Japanese patients with or without colorectal cancer |
title | Prevalence of pks-positive Escherichia coli in Japanese patients with or without colorectal cancer |
title_full | Prevalence of pks-positive Escherichia coli in Japanese patients with or without colorectal cancer |
title_fullStr | Prevalence of pks-positive Escherichia coli in Japanese patients with or without colorectal cancer |
title_full_unstemmed | Prevalence of pks-positive Escherichia coli in Japanese patients with or without colorectal cancer |
title_short | Prevalence of pks-positive Escherichia coli in Japanese patients with or without colorectal cancer |
title_sort | prevalence of pks-positive escherichia coli in japanese patients with or without colorectal cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5468999/ https://www.ncbi.nlm.nih.gov/pubmed/28616082 http://dx.doi.org/10.1186/s13099-017-0185-x |
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