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Evaluation of Gaussia luciferase and foot-and-mouth disease virus 2A translational interrupter chimeras as polycistronic reporters for transgene expression

BACKGROUND: The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity. We sought to simultaneously quantitate production of other recombinant proteins by transcript...

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Detalles Bibliográficos
Autores principales: Puckette, Michael, Burrage, Thomas, Neilan, John G., Rasmussen, Max
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469038/
https://www.ncbi.nlm.nih.gov/pubmed/28606077
http://dx.doi.org/10.1186/s12896-017-0367-0