Cargando…

Circulating endothelial microparticles and miR-92a in acute myocardial infarction

Microparticles (MPs) and miRNAs have been shown to play important roles in coronary artery disease (CAD) by monitoring endothelial dysfunction. The present study aims to investigate the diagnostic value of endothelial MPs (EMPs) and miRNAs (miR-92a or miR-23a) as biomarkers in distinguishing patient...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yuchen, Cheng, Junjun, Chen, Fang, Wu, Changyan, Zhang, Junmeng, Ren, Xuejun, Pan, Yu, Nie, Bin, Li, Quan, Li, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469331/
https://www.ncbi.nlm.nih.gov/pubmed/28213360
http://dx.doi.org/10.1042/BSR20170047
_version_ 1783243562798809088
author Zhang, Yuchen
Cheng, Junjun
Chen, Fang
Wu, Changyan
Zhang, Junmeng
Ren, Xuejun
Pan, Yu
Nie, Bin
Li, Quan
Li, Yu
author_facet Zhang, Yuchen
Cheng, Junjun
Chen, Fang
Wu, Changyan
Zhang, Junmeng
Ren, Xuejun
Pan, Yu
Nie, Bin
Li, Quan
Li, Yu
author_sort Zhang, Yuchen
collection PubMed
description Microparticles (MPs) and miRNAs have been shown to play important roles in coronary artery disease (CAD) by monitoring endothelial dysfunction. The present study aims to investigate the diagnostic value of endothelial MPs (EMPs) and miRNAs (miR-92a or miR-23a) as biomarkers in distinguishing patients with acute myocardial infarction (AMI) from those with CAD. Plasma samples from 37 patients with AMI, 42 patients with stable CAD (SCAD), and 35 healthy adults were collected for investigation in the present study. The numbers of CD31+/CD42b− MPs, CD31+/CD42b+ MPs, and CD31−/CD42b− MPs were measured by flow cytometry and the levels of miR-92a and miR-23a were analyzed using reverse transcription-quantitative PCR. Moreover, cardiac troponin I (cTnI) expression was detected by ELISA to serve as a routine diagnostic parameter. The number of CD31+/CD42b− was higher in AMI group than those in SCAD and healthy groups. Besides, the expression of miR-92a was higher in AMI group compared with two other groups. Furthermore, evidence showed that there was a positive correlation between the levels of CD31+/CD42b− MPs and miR-92a. Finally, the receiver operating characteristic (ROC) curve revealed that the area value under the curve of CD31+/CD42b− MPs, miR-92a and cTnI was 0.893, 0.888, and 0.912 respectively. CD31+/CD42b− MPs and miR-92a might have great potential to provide diagnostic value for AMI and could probably regulate the endothelial dysfunction in AMI patients.
format Online
Article
Text
id pubmed-5469331
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-54693312017-06-22 Circulating endothelial microparticles and miR-92a in acute myocardial infarction Zhang, Yuchen Cheng, Junjun Chen, Fang Wu, Changyan Zhang, Junmeng Ren, Xuejun Pan, Yu Nie, Bin Li, Quan Li, Yu Biosci Rep Research Articles Microparticles (MPs) and miRNAs have been shown to play important roles in coronary artery disease (CAD) by monitoring endothelial dysfunction. The present study aims to investigate the diagnostic value of endothelial MPs (EMPs) and miRNAs (miR-92a or miR-23a) as biomarkers in distinguishing patients with acute myocardial infarction (AMI) from those with CAD. Plasma samples from 37 patients with AMI, 42 patients with stable CAD (SCAD), and 35 healthy adults were collected for investigation in the present study. The numbers of CD31+/CD42b− MPs, CD31+/CD42b+ MPs, and CD31−/CD42b− MPs were measured by flow cytometry and the levels of miR-92a and miR-23a were analyzed using reverse transcription-quantitative PCR. Moreover, cardiac troponin I (cTnI) expression was detected by ELISA to serve as a routine diagnostic parameter. The number of CD31+/CD42b− was higher in AMI group than those in SCAD and healthy groups. Besides, the expression of miR-92a was higher in AMI group compared with two other groups. Furthermore, evidence showed that there was a positive correlation between the levels of CD31+/CD42b− MPs and miR-92a. Finally, the receiver operating characteristic (ROC) curve revealed that the area value under the curve of CD31+/CD42b− MPs, miR-92a and cTnI was 0.893, 0.888, and 0.912 respectively. CD31+/CD42b− MPs and miR-92a might have great potential to provide diagnostic value for AMI and could probably regulate the endothelial dysfunction in AMI patients. Portland Press Ltd. 2017-03-27 /pmc/articles/PMC5469331/ /pubmed/28213360 http://dx.doi.org/10.1042/BSR20170047 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Zhang, Yuchen
Cheng, Junjun
Chen, Fang
Wu, Changyan
Zhang, Junmeng
Ren, Xuejun
Pan, Yu
Nie, Bin
Li, Quan
Li, Yu
Circulating endothelial microparticles and miR-92a in acute myocardial infarction
title Circulating endothelial microparticles and miR-92a in acute myocardial infarction
title_full Circulating endothelial microparticles and miR-92a in acute myocardial infarction
title_fullStr Circulating endothelial microparticles and miR-92a in acute myocardial infarction
title_full_unstemmed Circulating endothelial microparticles and miR-92a in acute myocardial infarction
title_short Circulating endothelial microparticles and miR-92a in acute myocardial infarction
title_sort circulating endothelial microparticles and mir-92a in acute myocardial infarction
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469331/
https://www.ncbi.nlm.nih.gov/pubmed/28213360
http://dx.doi.org/10.1042/BSR20170047
work_keys_str_mv AT zhangyuchen circulatingendothelialmicroparticlesandmir92ainacutemyocardialinfarction
AT chengjunjun circulatingendothelialmicroparticlesandmir92ainacutemyocardialinfarction
AT chenfang circulatingendothelialmicroparticlesandmir92ainacutemyocardialinfarction
AT wuchangyan circulatingendothelialmicroparticlesandmir92ainacutemyocardialinfarction
AT zhangjunmeng circulatingendothelialmicroparticlesandmir92ainacutemyocardialinfarction
AT renxuejun circulatingendothelialmicroparticlesandmir92ainacutemyocardialinfarction
AT panyu circulatingendothelialmicroparticlesandmir92ainacutemyocardialinfarction
AT niebin circulatingendothelialmicroparticlesandmir92ainacutemyocardialinfarction
AT liquan circulatingendothelialmicroparticlesandmir92ainacutemyocardialinfarction
AT liyu circulatingendothelialmicroparticlesandmir92ainacutemyocardialinfarction