Cargando…

Evaluation of subcutaneous infiltration of autologous platelet-rich plasma on skin-wound healing in dogs

Platelet-rich plasma (PRP) is known to be rich in growth factors and cytokines, which are crucial to the healing process. This study investigate the effect of subcutaneous (S/C) infiltration of autologous PRP at the wound boundaries on wound epithelization and contraction. Five adult male mongrel do...

Descripción completa

Detalles Bibliográficos
Autores principales: Farghali, Haithem A., AbdElKader, Naglaa A., Khattab, Marwa S., AbuBakr, Huda O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469334/
https://www.ncbi.nlm.nih.gov/pubmed/28246352
http://dx.doi.org/10.1042/BSR20160503
_version_ 1783243563588386816
author Farghali, Haithem A.
AbdElKader, Naglaa A.
Khattab, Marwa S.
AbuBakr, Huda O.
author_facet Farghali, Haithem A.
AbdElKader, Naglaa A.
Khattab, Marwa S.
AbuBakr, Huda O.
author_sort Farghali, Haithem A.
collection PubMed
description Platelet-rich plasma (PRP) is known to be rich in growth factors and cytokines, which are crucial to the healing process. This study investigate the effect of subcutaneous (S/C) infiltration of autologous PRP at the wound boundaries on wound epithelization and contraction. Five adult male mongrel dogs were used. Bilateral acute full thickness skin wounds (3 cm diameter) were created on the thorax symmetrically. Right side wounds were subcutaneously infiltrated with activated PRP at day 0 and then every week for three consecutive weeks. The left wound was left as control. Wound contraction and epithelization were clinically evaluated. Expression of collagen type I (COLI) A2, (COLIA2),histopathology and immunohistochemical (IHC) staining of COLI α1 (COLIA1) were performed on skin biopsies at first, second and third weeks. The catalase activity, malondialdehyde (MDA) concentration and matrix metalloproteinase (MMP) 9 (MMP-9) activity were assessed in wound fluid samples. All data were analysed statistically. The epithelization percent significantly increased in the PRP-treated wound at week 3. Collagen was well organized in the PRP-treated wounds compared with control wounds at week 3. The COLIA2 expression and intensity of COLIA1 significantly increased in PRP-treated wounds. MDA concentration was significantly decreased in PRP-treated wound at week 3. The catalase activity exhibited no difference between PRP treated and untreated wounds. The activity of MMP-9 reached its peak at the second week and was significantly high in the PRP-treated group. S/C infiltration of autologous PRP at the wound margins enhances the wound epithelization and reduces the scar tissue formation.
format Online
Article
Text
id pubmed-5469334
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-54693342017-06-22 Evaluation of subcutaneous infiltration of autologous platelet-rich plasma on skin-wound healing in dogs Farghali, Haithem A. AbdElKader, Naglaa A. Khattab, Marwa S. AbuBakr, Huda O. Biosci Rep Research Articles Platelet-rich plasma (PRP) is known to be rich in growth factors and cytokines, which are crucial to the healing process. This study investigate the effect of subcutaneous (S/C) infiltration of autologous PRP at the wound boundaries on wound epithelization and contraction. Five adult male mongrel dogs were used. Bilateral acute full thickness skin wounds (3 cm diameter) were created on the thorax symmetrically. Right side wounds were subcutaneously infiltrated with activated PRP at day 0 and then every week for three consecutive weeks. The left wound was left as control. Wound contraction and epithelization were clinically evaluated. Expression of collagen type I (COLI) A2, (COLIA2),histopathology and immunohistochemical (IHC) staining of COLI α1 (COLIA1) were performed on skin biopsies at first, second and third weeks. The catalase activity, malondialdehyde (MDA) concentration and matrix metalloproteinase (MMP) 9 (MMP-9) activity were assessed in wound fluid samples. All data were analysed statistically. The epithelization percent significantly increased in the PRP-treated wound at week 3. Collagen was well organized in the PRP-treated wounds compared with control wounds at week 3. The COLIA2 expression and intensity of COLIA1 significantly increased in PRP-treated wounds. MDA concentration was significantly decreased in PRP-treated wound at week 3. The catalase activity exhibited no difference between PRP treated and untreated wounds. The activity of MMP-9 reached its peak at the second week and was significantly high in the PRP-treated group. S/C infiltration of autologous PRP at the wound margins enhances the wound epithelization and reduces the scar tissue formation. Portland Press Ltd. 2017-04-10 /pmc/articles/PMC5469334/ /pubmed/28246352 http://dx.doi.org/10.1042/BSR20160503 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Farghali, Haithem A.
AbdElKader, Naglaa A.
Khattab, Marwa S.
AbuBakr, Huda O.
Evaluation of subcutaneous infiltration of autologous platelet-rich plasma on skin-wound healing in dogs
title Evaluation of subcutaneous infiltration of autologous platelet-rich plasma on skin-wound healing in dogs
title_full Evaluation of subcutaneous infiltration of autologous platelet-rich plasma on skin-wound healing in dogs
title_fullStr Evaluation of subcutaneous infiltration of autologous platelet-rich plasma on skin-wound healing in dogs
title_full_unstemmed Evaluation of subcutaneous infiltration of autologous platelet-rich plasma on skin-wound healing in dogs
title_short Evaluation of subcutaneous infiltration of autologous platelet-rich plasma on skin-wound healing in dogs
title_sort evaluation of subcutaneous infiltration of autologous platelet-rich plasma on skin-wound healing in dogs
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469334/
https://www.ncbi.nlm.nih.gov/pubmed/28246352
http://dx.doi.org/10.1042/BSR20160503
work_keys_str_mv AT farghalihaithema evaluationofsubcutaneousinfiltrationofautologousplateletrichplasmaonskinwoundhealingindogs
AT abdelkadernaglaaa evaluationofsubcutaneousinfiltrationofautologousplateletrichplasmaonskinwoundhealingindogs
AT khattabmarwas evaluationofsubcutaneousinfiltrationofautologousplateletrichplasmaonskinwoundhealingindogs
AT abubakrhudao evaluationofsubcutaneousinfiltrationofautologousplateletrichplasmaonskinwoundhealingindogs