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Recombination-Mediated Host Adaptation by Avian Staphylococcus aureus

Staphylococcus aureus are globally disseminated among farmed chickens causing skeletal muscle infections, dermatitis, and septicaemia. The emergence of poultry-associated lineages has involved zoonotic transmission from humans to chickens but questions remain about the specific adaptations that prom...

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Autores principales: Murray, Susan, Pascoe, Ben, Méric, Guillaume, Mageiros, Leonardos, Yahara, Koji, Hitchings, Matthew D., Friedmann, Yasmin, Wilkinson, Thomas S., Gormley, Fraser J., Mack, Dietrich, Bray, James E., Lamble, Sarah, Bowden, Rory, Jolley, Keith A., Maiden, Martin C.J., Wendlandt, Sarah, Schwarz, Stefan, Corander, Jukka, Fitzgerald, J. Ross, Sheppard, Samuel K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469444/
https://www.ncbi.nlm.nih.gov/pubmed/28338786
http://dx.doi.org/10.1093/gbe/evx037
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author Murray, Susan
Pascoe, Ben
Méric, Guillaume
Mageiros, Leonardos
Yahara, Koji
Hitchings, Matthew D.
Friedmann, Yasmin
Wilkinson, Thomas S.
Gormley, Fraser J.
Mack, Dietrich
Bray, James E.
Lamble, Sarah
Bowden, Rory
Jolley, Keith A.
Maiden, Martin C.J.
Wendlandt, Sarah
Schwarz, Stefan
Corander, Jukka
Fitzgerald, J. Ross
Sheppard, Samuel K.
author_facet Murray, Susan
Pascoe, Ben
Méric, Guillaume
Mageiros, Leonardos
Yahara, Koji
Hitchings, Matthew D.
Friedmann, Yasmin
Wilkinson, Thomas S.
Gormley, Fraser J.
Mack, Dietrich
Bray, James E.
Lamble, Sarah
Bowden, Rory
Jolley, Keith A.
Maiden, Martin C.J.
Wendlandt, Sarah
Schwarz, Stefan
Corander, Jukka
Fitzgerald, J. Ross
Sheppard, Samuel K.
author_sort Murray, Susan
collection PubMed
description Staphylococcus aureus are globally disseminated among farmed chickens causing skeletal muscle infections, dermatitis, and septicaemia. The emergence of poultry-associated lineages has involved zoonotic transmission from humans to chickens but questions remain about the specific adaptations that promote proliferation of chicken pathogens. We characterized genetic variation in a population of genome-sequenced S. aureus isolates of poultry and human origin. Genealogical analysis identified a dominant poultry-associated sequence cluster within the CC5 clonal complex. Poultry and human CC5 isolates were significantly distinct from each other and more recombination events were detected in the poultry isolates. We identified 44 recombination events in 33 genes along the branch extending to the poultry-specific CC5 cluster, and 47 genes were found more often in CC5 poultry isolates compared with those from humans. Many of these gene sequences were common in chicken isolates from other clonal complexes suggesting horizontal gene transfer among poultry associated lineages. Consistent with functional predictions for putative poultry-associated genes, poultry isolates showed enhanced growth at 42 °C and greater erythrocyte lysis on chicken blood agar in comparison with human isolates. By combining phenotype information with evolutionary analyses of staphylococcal genomes, we provide evidence of adaptation, following a human-to-poultry host transition. This has important implications for the emergence and dissemination of new pathogenic clones associated with modern agriculture.
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spelling pubmed-54694442017-06-21 Recombination-Mediated Host Adaptation by Avian Staphylococcus aureus Murray, Susan Pascoe, Ben Méric, Guillaume Mageiros, Leonardos Yahara, Koji Hitchings, Matthew D. Friedmann, Yasmin Wilkinson, Thomas S. Gormley, Fraser J. Mack, Dietrich Bray, James E. Lamble, Sarah Bowden, Rory Jolley, Keith A. Maiden, Martin C.J. Wendlandt, Sarah Schwarz, Stefan Corander, Jukka Fitzgerald, J. Ross Sheppard, Samuel K. Genome Biol Evol Research Article Staphylococcus aureus are globally disseminated among farmed chickens causing skeletal muscle infections, dermatitis, and septicaemia. The emergence of poultry-associated lineages has involved zoonotic transmission from humans to chickens but questions remain about the specific adaptations that promote proliferation of chicken pathogens. We characterized genetic variation in a population of genome-sequenced S. aureus isolates of poultry and human origin. Genealogical analysis identified a dominant poultry-associated sequence cluster within the CC5 clonal complex. Poultry and human CC5 isolates were significantly distinct from each other and more recombination events were detected in the poultry isolates. We identified 44 recombination events in 33 genes along the branch extending to the poultry-specific CC5 cluster, and 47 genes were found more often in CC5 poultry isolates compared with those from humans. Many of these gene sequences were common in chicken isolates from other clonal complexes suggesting horizontal gene transfer among poultry associated lineages. Consistent with functional predictions for putative poultry-associated genes, poultry isolates showed enhanced growth at 42 °C and greater erythrocyte lysis on chicken blood agar in comparison with human isolates. By combining phenotype information with evolutionary analyses of staphylococcal genomes, we provide evidence of adaptation, following a human-to-poultry host transition. This has important implications for the emergence and dissemination of new pathogenic clones associated with modern agriculture. Oxford University Press 2017-04-01 /pmc/articles/PMC5469444/ /pubmed/28338786 http://dx.doi.org/10.1093/gbe/evx037 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Murray, Susan
Pascoe, Ben
Méric, Guillaume
Mageiros, Leonardos
Yahara, Koji
Hitchings, Matthew D.
Friedmann, Yasmin
Wilkinson, Thomas S.
Gormley, Fraser J.
Mack, Dietrich
Bray, James E.
Lamble, Sarah
Bowden, Rory
Jolley, Keith A.
Maiden, Martin C.J.
Wendlandt, Sarah
Schwarz, Stefan
Corander, Jukka
Fitzgerald, J. Ross
Sheppard, Samuel K.
Recombination-Mediated Host Adaptation by Avian Staphylococcus aureus
title Recombination-Mediated Host Adaptation by Avian Staphylococcus aureus
title_full Recombination-Mediated Host Adaptation by Avian Staphylococcus aureus
title_fullStr Recombination-Mediated Host Adaptation by Avian Staphylococcus aureus
title_full_unstemmed Recombination-Mediated Host Adaptation by Avian Staphylococcus aureus
title_short Recombination-Mediated Host Adaptation by Avian Staphylococcus aureus
title_sort recombination-mediated host adaptation by avian staphylococcus aureus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469444/
https://www.ncbi.nlm.nih.gov/pubmed/28338786
http://dx.doi.org/10.1093/gbe/evx037
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