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Screening Genotoxicity Chemistry with Microfluidic Electrochemiluminescent Arrays

This review describes progress in the development of electrochemiluminescent (ECL) arrays aimed at sensing DNA damage to identify genotoxic chemistry related to reactive metabolites. Genotoxicity refers to chemical or photochemical processes that damage DNA with toxic consequences. Our arrays featur...

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Detalles Bibliográficos
Autores principales: Bist, Itti, Bano, Kiran, Rusling, James F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469531/
https://www.ncbi.nlm.nih.gov/pubmed/28467352
http://dx.doi.org/10.3390/s17051008
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author Bist, Itti
Bano, Kiran
Rusling, James F.
author_facet Bist, Itti
Bano, Kiran
Rusling, James F.
author_sort Bist, Itti
collection PubMed
description This review describes progress in the development of electrochemiluminescent (ECL) arrays aimed at sensing DNA damage to identify genotoxic chemistry related to reactive metabolites. Genotoxicity refers to chemical or photochemical processes that damage DNA with toxic consequences. Our arrays feature DNA/enzyme films that form reactive metabolites of test chemicals that can subsequently react with DNA, thus enabling prediction of genotoxic chemical reactions. These high-throughput ECL arrays incorporating representative cohorts of human metabolic enzymes provide a platform for determining chemical toxicity profiles of new drug and environmental chemical candidates. The arrays can be designed to identify enzymes and enzyme cascades that produce the reactive metabolites. We also describe ECL arrays that detect oxidative DNA damage caused by metabolite-mediated reactive oxygen species. These approaches provide valuable high-throughput tools to complement modern toxicity bioassays and provide a more complete toxicity prediction for drug and chemical product development.
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spelling pubmed-54695312017-06-16 Screening Genotoxicity Chemistry with Microfluidic Electrochemiluminescent Arrays Bist, Itti Bano, Kiran Rusling, James F. Sensors (Basel) Review This review describes progress in the development of electrochemiluminescent (ECL) arrays aimed at sensing DNA damage to identify genotoxic chemistry related to reactive metabolites. Genotoxicity refers to chemical or photochemical processes that damage DNA with toxic consequences. Our arrays feature DNA/enzyme films that form reactive metabolites of test chemicals that can subsequently react with DNA, thus enabling prediction of genotoxic chemical reactions. These high-throughput ECL arrays incorporating representative cohorts of human metabolic enzymes provide a platform for determining chemical toxicity profiles of new drug and environmental chemical candidates. The arrays can be designed to identify enzymes and enzyme cascades that produce the reactive metabolites. We also describe ECL arrays that detect oxidative DNA damage caused by metabolite-mediated reactive oxygen species. These approaches provide valuable high-throughput tools to complement modern toxicity bioassays and provide a more complete toxicity prediction for drug and chemical product development. MDPI 2017-05-03 /pmc/articles/PMC5469531/ /pubmed/28467352 http://dx.doi.org/10.3390/s17051008 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Bist, Itti
Bano, Kiran
Rusling, James F.
Screening Genotoxicity Chemistry with Microfluidic Electrochemiluminescent Arrays
title Screening Genotoxicity Chemistry with Microfluidic Electrochemiluminescent Arrays
title_full Screening Genotoxicity Chemistry with Microfluidic Electrochemiluminescent Arrays
title_fullStr Screening Genotoxicity Chemistry with Microfluidic Electrochemiluminescent Arrays
title_full_unstemmed Screening Genotoxicity Chemistry with Microfluidic Electrochemiluminescent Arrays
title_short Screening Genotoxicity Chemistry with Microfluidic Electrochemiluminescent Arrays
title_sort screening genotoxicity chemistry with microfluidic electrochemiluminescent arrays
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469531/
https://www.ncbi.nlm.nih.gov/pubmed/28467352
http://dx.doi.org/10.3390/s17051008
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