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Raman micro-spectroscopy as a viable tool to monitor and estimate the ionic transport in epithelial cells

The typical response to the lowering of plasma Na(+) concentration and blood pressure in our body involves the release of aldosterone from the adrenal glands, which triggers the reabsorption of sodium in the kidney. Although the effects of aldosterone on this physiological mechanism were extensively...

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Autores principales: Puppulin, Leonardo, Pezzotti, Giuseppe, Sun, Hongxin, Hosogi, Shigekuni, Nakahari, Takashi, Inui, Toshio, Kumamoto, Yasuaki, Tanaka, Hideo, Marunaka, Yoshinori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469862/
https://www.ncbi.nlm.nih.gov/pubmed/28611439
http://dx.doi.org/10.1038/s41598-017-03595-y
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author Puppulin, Leonardo
Pezzotti, Giuseppe
Sun, Hongxin
Hosogi, Shigekuni
Nakahari, Takashi
Inui, Toshio
Kumamoto, Yasuaki
Tanaka, Hideo
Marunaka, Yoshinori
author_facet Puppulin, Leonardo
Pezzotti, Giuseppe
Sun, Hongxin
Hosogi, Shigekuni
Nakahari, Takashi
Inui, Toshio
Kumamoto, Yasuaki
Tanaka, Hideo
Marunaka, Yoshinori
author_sort Puppulin, Leonardo
collection PubMed
description The typical response to the lowering of plasma Na(+) concentration and blood pressure in our body involves the release of aldosterone from the adrenal glands, which triggers the reabsorption of sodium in the kidney. Although the effects of aldosterone on this physiological mechanism were extensively studied in the past decades, there are still some aspects to be fully elucidated. In the present study, we propose for the first time a new approach based on Raman spectroscopy to monitor the ionic activity in aldosterone-treated A6 renal epithelial cells. This spectroscopic technique is capable of probing the cells through their thickness in a non-destructive and nimble way. The spectroscopic variations of the Raman bands associated to the O-H stretching of water were correlated to the variations of ionic concentration in the intracellular and extracellular fluids. The increase of Na(+) concentration gradients was clearly visualized in the cytosol of aldosterone-treated cells. The enhancement of the Na(+) current density induced by aldosterone was estimated from the variation of the ionic chemical potential across the intracellular space. In addition, the variation of the O-H Raman bands of water was used to quantify the cell thickness, which was not affected by aldosterone.
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spelling pubmed-54698622017-06-19 Raman micro-spectroscopy as a viable tool to monitor and estimate the ionic transport in epithelial cells Puppulin, Leonardo Pezzotti, Giuseppe Sun, Hongxin Hosogi, Shigekuni Nakahari, Takashi Inui, Toshio Kumamoto, Yasuaki Tanaka, Hideo Marunaka, Yoshinori Sci Rep Article The typical response to the lowering of plasma Na(+) concentration and blood pressure in our body involves the release of aldosterone from the adrenal glands, which triggers the reabsorption of sodium in the kidney. Although the effects of aldosterone on this physiological mechanism were extensively studied in the past decades, there are still some aspects to be fully elucidated. In the present study, we propose for the first time a new approach based on Raman spectroscopy to monitor the ionic activity in aldosterone-treated A6 renal epithelial cells. This spectroscopic technique is capable of probing the cells through their thickness in a non-destructive and nimble way. The spectroscopic variations of the Raman bands associated to the O-H stretching of water were correlated to the variations of ionic concentration in the intracellular and extracellular fluids. The increase of Na(+) concentration gradients was clearly visualized in the cytosol of aldosterone-treated cells. The enhancement of the Na(+) current density induced by aldosterone was estimated from the variation of the ionic chemical potential across the intracellular space. In addition, the variation of the O-H Raman bands of water was used to quantify the cell thickness, which was not affected by aldosterone. Nature Publishing Group UK 2017-06-13 /pmc/articles/PMC5469862/ /pubmed/28611439 http://dx.doi.org/10.1038/s41598-017-03595-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Puppulin, Leonardo
Pezzotti, Giuseppe
Sun, Hongxin
Hosogi, Shigekuni
Nakahari, Takashi
Inui, Toshio
Kumamoto, Yasuaki
Tanaka, Hideo
Marunaka, Yoshinori
Raman micro-spectroscopy as a viable tool to monitor and estimate the ionic transport in epithelial cells
title Raman micro-spectroscopy as a viable tool to monitor and estimate the ionic transport in epithelial cells
title_full Raman micro-spectroscopy as a viable tool to monitor and estimate the ionic transport in epithelial cells
title_fullStr Raman micro-spectroscopy as a viable tool to monitor and estimate the ionic transport in epithelial cells
title_full_unstemmed Raman micro-spectroscopy as a viable tool to monitor and estimate the ionic transport in epithelial cells
title_short Raman micro-spectroscopy as a viable tool to monitor and estimate the ionic transport in epithelial cells
title_sort raman micro-spectroscopy as a viable tool to monitor and estimate the ionic transport in epithelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469862/
https://www.ncbi.nlm.nih.gov/pubmed/28611439
http://dx.doi.org/10.1038/s41598-017-03595-y
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