Cargando…

Viral Delivery of dsRNA for Control of Insect Agricultural Pests and Vectors of Human Disease: Prospects and Challenges

RNAi is applied as a new and safe method for pest control in agriculture but efficiency and specificity of delivery of dsRNA trigger remains a critical issue. Various agents have been proposed to augment dsRNA delivery, such as engineered micro-organisms and synthetic nanoparticles, but the use of v...

Descripción completa

Detalles Bibliográficos
Autores principales: Kolliopoulou, Anna, Taning, Clauvis N. T., Smagghe, Guy, Swevers, Luc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469917/
https://www.ncbi.nlm.nih.gov/pubmed/28659820
http://dx.doi.org/10.3389/fphys.2017.00399
_version_ 1783243669098201088
author Kolliopoulou, Anna
Taning, Clauvis N. T.
Smagghe, Guy
Swevers, Luc
author_facet Kolliopoulou, Anna
Taning, Clauvis N. T.
Smagghe, Guy
Swevers, Luc
author_sort Kolliopoulou, Anna
collection PubMed
description RNAi is applied as a new and safe method for pest control in agriculture but efficiency and specificity of delivery of dsRNA trigger remains a critical issue. Various agents have been proposed to augment dsRNA delivery, such as engineered micro-organisms and synthetic nanoparticles, but the use of viruses has received relatively little attention. Here we present a critical view of the potential of the use of recombinant viruses for efficient and specific delivery of dsRNA. First of all, it requires the availability of plasmid-based reverse genetics systems for virus production, of which an overview is presented. For RNA viruses, their application seems to be straightforward since dsRNA is produced as an intermediate molecule during viral replication, but DNA viruses also have potential through the production of RNA hairpins after transcription. However, application of recombinant virus for dsRNA delivery may not be straightforward in many cases, since viruses can encode RNAi suppressors, and virus-induced silencing effects can be determined by the properties of the encoded RNAi suppressor. An alternative is virus-like particles that retain the efficiency and specificity determinants of natural virions but have encapsidated non-replicating RNA. Finally, the use of viruses raises important safety issues which need to be addressed before application can proceed.
format Online
Article
Text
id pubmed-5469917
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-54699172017-06-28 Viral Delivery of dsRNA for Control of Insect Agricultural Pests and Vectors of Human Disease: Prospects and Challenges Kolliopoulou, Anna Taning, Clauvis N. T. Smagghe, Guy Swevers, Luc Front Physiol Physiology RNAi is applied as a new and safe method for pest control in agriculture but efficiency and specificity of delivery of dsRNA trigger remains a critical issue. Various agents have been proposed to augment dsRNA delivery, such as engineered micro-organisms and synthetic nanoparticles, but the use of viruses has received relatively little attention. Here we present a critical view of the potential of the use of recombinant viruses for efficient and specific delivery of dsRNA. First of all, it requires the availability of plasmid-based reverse genetics systems for virus production, of which an overview is presented. For RNA viruses, their application seems to be straightforward since dsRNA is produced as an intermediate molecule during viral replication, but DNA viruses also have potential through the production of RNA hairpins after transcription. However, application of recombinant virus for dsRNA delivery may not be straightforward in many cases, since viruses can encode RNAi suppressors, and virus-induced silencing effects can be determined by the properties of the encoded RNAi suppressor. An alternative is virus-like particles that retain the efficiency and specificity determinants of natural virions but have encapsidated non-replicating RNA. Finally, the use of viruses raises important safety issues which need to be addressed before application can proceed. Frontiers Media S.A. 2017-06-14 /pmc/articles/PMC5469917/ /pubmed/28659820 http://dx.doi.org/10.3389/fphys.2017.00399 Text en Copyright © 2017 Kolliopoulou, Taning, Smagghe and Swevers. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Kolliopoulou, Anna
Taning, Clauvis N. T.
Smagghe, Guy
Swevers, Luc
Viral Delivery of dsRNA for Control of Insect Agricultural Pests and Vectors of Human Disease: Prospects and Challenges
title Viral Delivery of dsRNA for Control of Insect Agricultural Pests and Vectors of Human Disease: Prospects and Challenges
title_full Viral Delivery of dsRNA for Control of Insect Agricultural Pests and Vectors of Human Disease: Prospects and Challenges
title_fullStr Viral Delivery of dsRNA for Control of Insect Agricultural Pests and Vectors of Human Disease: Prospects and Challenges
title_full_unstemmed Viral Delivery of dsRNA for Control of Insect Agricultural Pests and Vectors of Human Disease: Prospects and Challenges
title_short Viral Delivery of dsRNA for Control of Insect Agricultural Pests and Vectors of Human Disease: Prospects and Challenges
title_sort viral delivery of dsrna for control of insect agricultural pests and vectors of human disease: prospects and challenges
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469917/
https://www.ncbi.nlm.nih.gov/pubmed/28659820
http://dx.doi.org/10.3389/fphys.2017.00399
work_keys_str_mv AT kolliopoulouanna viraldeliveryofdsrnaforcontrolofinsectagriculturalpestsandvectorsofhumandiseaseprospectsandchallenges
AT taningclauvisnt viraldeliveryofdsrnaforcontrolofinsectagriculturalpestsandvectorsofhumandiseaseprospectsandchallenges
AT smaggheguy viraldeliveryofdsrnaforcontrolofinsectagriculturalpestsandvectorsofhumandiseaseprospectsandchallenges
AT sweversluc viraldeliveryofdsrnaforcontrolofinsectagriculturalpestsandvectorsofhumandiseaseprospectsandchallenges