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Comprehensive Monosynaptic Rabies Virus Mapping of Host Connectivity with Neural Progenitor Grafts after Spinal Cord Injury

Neural progenitor cells grafted to sites of spinal cord injury have supported electrophysiological and functional recovery in several studies. Mechanisms associated with graft-related improvements in outcome appear dependent on functional synaptic integration of graft and host systems, although the...

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Autores principales: Adler, Andrew F., Lee-Kubli, Corinne, Kumamaru, Hiromi, Kadoya, Ken, Tuszynski, Mark H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469919/
https://www.ncbi.nlm.nih.gov/pubmed/28479302
http://dx.doi.org/10.1016/j.stemcr.2017.04.004
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author Adler, Andrew F.
Lee-Kubli, Corinne
Kumamaru, Hiromi
Kadoya, Ken
Tuszynski, Mark H.
author_facet Adler, Andrew F.
Lee-Kubli, Corinne
Kumamaru, Hiromi
Kadoya, Ken
Tuszynski, Mark H.
author_sort Adler, Andrew F.
collection PubMed
description Neural progenitor cells grafted to sites of spinal cord injury have supported electrophysiological and functional recovery in several studies. Mechanisms associated with graft-related improvements in outcome appear dependent on functional synaptic integration of graft and host systems, although the extent and diversity of synaptic integration of grafts with hosts are unknown. Using transgenic mouse spinal neural progenitor cell grafts expressing the TVA and G-protein components of the modified rabies virus system, we initiated monosynaptic tracing strictly from graft neurons placed in sites of cervical spinal cord injury. We find that graft neurons receive synaptic inputs from virtually every known host system that normally innervates the spinal cord, including numerous cortical, brainstem, spinal cord, and dorsal root ganglia inputs. Thus, implanted neural progenitor cells receive an extensive range of host neural inputs to the injury site, potentially enabling functional restoration across multiple systems.
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spelling pubmed-54699192017-06-23 Comprehensive Monosynaptic Rabies Virus Mapping of Host Connectivity with Neural Progenitor Grafts after Spinal Cord Injury Adler, Andrew F. Lee-Kubli, Corinne Kumamaru, Hiromi Kadoya, Ken Tuszynski, Mark H. Stem Cell Reports Report Neural progenitor cells grafted to sites of spinal cord injury have supported electrophysiological and functional recovery in several studies. Mechanisms associated with graft-related improvements in outcome appear dependent on functional synaptic integration of graft and host systems, although the extent and diversity of synaptic integration of grafts with hosts are unknown. Using transgenic mouse spinal neural progenitor cell grafts expressing the TVA and G-protein components of the modified rabies virus system, we initiated monosynaptic tracing strictly from graft neurons placed in sites of cervical spinal cord injury. We find that graft neurons receive synaptic inputs from virtually every known host system that normally innervates the spinal cord, including numerous cortical, brainstem, spinal cord, and dorsal root ganglia inputs. Thus, implanted neural progenitor cells receive an extensive range of host neural inputs to the injury site, potentially enabling functional restoration across multiple systems. Elsevier 2017-05-04 /pmc/articles/PMC5469919/ /pubmed/28479302 http://dx.doi.org/10.1016/j.stemcr.2017.04.004 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Report
Adler, Andrew F.
Lee-Kubli, Corinne
Kumamaru, Hiromi
Kadoya, Ken
Tuszynski, Mark H.
Comprehensive Monosynaptic Rabies Virus Mapping of Host Connectivity with Neural Progenitor Grafts after Spinal Cord Injury
title Comprehensive Monosynaptic Rabies Virus Mapping of Host Connectivity with Neural Progenitor Grafts after Spinal Cord Injury
title_full Comprehensive Monosynaptic Rabies Virus Mapping of Host Connectivity with Neural Progenitor Grafts after Spinal Cord Injury
title_fullStr Comprehensive Monosynaptic Rabies Virus Mapping of Host Connectivity with Neural Progenitor Grafts after Spinal Cord Injury
title_full_unstemmed Comprehensive Monosynaptic Rabies Virus Mapping of Host Connectivity with Neural Progenitor Grafts after Spinal Cord Injury
title_short Comprehensive Monosynaptic Rabies Virus Mapping of Host Connectivity with Neural Progenitor Grafts after Spinal Cord Injury
title_sort comprehensive monosynaptic rabies virus mapping of host connectivity with neural progenitor grafts after spinal cord injury
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469919/
https://www.ncbi.nlm.nih.gov/pubmed/28479302
http://dx.doi.org/10.1016/j.stemcr.2017.04.004
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