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The Effects of Physiological and Methodological Determinants on (18)F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model

INTRODUCTION: In this study, the influence of physiological determinants on 18F-fluoro-d-glucose ((18)F-FDG) brain uptake was evaluated in a mouse model of Alzheimer disease. MATERIALS AND METHODS: TASTPM (Tg) and age-matched C57BL/6 J (WT) mice were fasted for 10 hours, while another group was fast...

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Autores principales: Deleye, Steven, Waldron, Ann-Marie, Richardson, Jill C., Schmidt, Mark, Langlois, Xavier, Stroobants, Sigrid, Staelens, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470082/
https://www.ncbi.nlm.nih.gov/pubmed/27030402
http://dx.doi.org/10.1177/1536012115624919
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author Deleye, Steven
Waldron, Ann-Marie
Richardson, Jill C.
Schmidt, Mark
Langlois, Xavier
Stroobants, Sigrid
Staelens, Steven
author_facet Deleye, Steven
Waldron, Ann-Marie
Richardson, Jill C.
Schmidt, Mark
Langlois, Xavier
Stroobants, Sigrid
Staelens, Steven
author_sort Deleye, Steven
collection PubMed
description INTRODUCTION: In this study, the influence of physiological determinants on 18F-fluoro-d-glucose ((18)F-FDG) brain uptake was evaluated in a mouse model of Alzheimer disease. MATERIALS AND METHODS: TASTPM (Tg) and age-matched C57BL/6 J (WT) mice were fasted for 10 hours, while another group was fasted for 20 hours to evaluate the effect of fasting duration. The effect of repeatedly scanning was evaluated by scanning Tg and WT mice at days 1, 4, and 7. Brain (18)F-FDG uptake was evaluated in the thalamus being the most indicative region. Finally, the cerebellum was tested as a reference region for the relative standard uptake value (rSUV). RESULTS: When correcting the brain uptake for glucose, the effect of different fasting durations was attenuated and the anticipated hypometabolism in Tg mice was demonstrated. Also, with repeated scanning, the brain uptake values within a group and the hypometabolism of the Tg mice only remained stable over time when glucose correction was applied. Finally, hypometabolism was also observed in the cerebellum, yielding artificially higher rSUV values for Tg mice. CONCLUSION: Corrections for blood glucose levels have to be applied when semiquantifying (18)F-FDG brain uptake in mouse models for AD. Potential reference regions for normalization should be thoroughly investigated to ensure that they are not pathologically affected also by afferent connections.
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spelling pubmed-54700822017-06-22 The Effects of Physiological and Methodological Determinants on (18)F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model Deleye, Steven Waldron, Ann-Marie Richardson, Jill C. Schmidt, Mark Langlois, Xavier Stroobants, Sigrid Staelens, Steven Mol Imaging Research Articles INTRODUCTION: In this study, the influence of physiological determinants on 18F-fluoro-d-glucose ((18)F-FDG) brain uptake was evaluated in a mouse model of Alzheimer disease. MATERIALS AND METHODS: TASTPM (Tg) and age-matched C57BL/6 J (WT) mice were fasted for 10 hours, while another group was fasted for 20 hours to evaluate the effect of fasting duration. The effect of repeatedly scanning was evaluated by scanning Tg and WT mice at days 1, 4, and 7. Brain (18)F-FDG uptake was evaluated in the thalamus being the most indicative region. Finally, the cerebellum was tested as a reference region for the relative standard uptake value (rSUV). RESULTS: When correcting the brain uptake for glucose, the effect of different fasting durations was attenuated and the anticipated hypometabolism in Tg mice was demonstrated. Also, with repeated scanning, the brain uptake values within a group and the hypometabolism of the Tg mice only remained stable over time when glucose correction was applied. Finally, hypometabolism was also observed in the cerebellum, yielding artificially higher rSUV values for Tg mice. CONCLUSION: Corrections for blood glucose levels have to be applied when semiquantifying (18)F-FDG brain uptake in mouse models for AD. Potential reference regions for normalization should be thoroughly investigated to ensure that they are not pathologically affected also by afferent connections. SAGE Publications 2016-02-11 /pmc/articles/PMC5470082/ /pubmed/27030402 http://dx.doi.org/10.1177/1536012115624919 Text en © The Author(s) 2016 http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Research Articles
Deleye, Steven
Waldron, Ann-Marie
Richardson, Jill C.
Schmidt, Mark
Langlois, Xavier
Stroobants, Sigrid
Staelens, Steven
The Effects of Physiological and Methodological Determinants on (18)F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model
title The Effects of Physiological and Methodological Determinants on (18)F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model
title_full The Effects of Physiological and Methodological Determinants on (18)F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model
title_fullStr The Effects of Physiological and Methodological Determinants on (18)F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model
title_full_unstemmed The Effects of Physiological and Methodological Determinants on (18)F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model
title_short The Effects of Physiological and Methodological Determinants on (18)F-FDG Mouse Brain Imaging Exemplified in a Double Transgenic Alzheimer Model
title_sort effects of physiological and methodological determinants on (18)f-fdg mouse brain imaging exemplified in a double transgenic alzheimer model
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470082/
https://www.ncbi.nlm.nih.gov/pubmed/27030402
http://dx.doi.org/10.1177/1536012115624919
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