CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion

CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of β-catenin exon 3, which encodes an autoinhibito...

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Detalles Bibliográficos
Autores principales: Mou, Haiwei, Smith, Jordan L., Peng, Lingtao, Yin, Hao, Moore, Jill, Zhang, Xiao-Ou, Song, Chun-Qing, Sheel, Ankur, Wu, Qiongqiong, Ozata, Deniz M., Li, Yingxiang, Anderson, Daniel G., Emerson, Charles P., Sontheimer, Erik J., Moore, Melissa J., Weng, Zhiping, Xue, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470253/
https://www.ncbi.nlm.nih.gov/pubmed/28615073
http://dx.doi.org/10.1186/s13059-017-1237-8

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