Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome

Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain...

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Autores principales: Martins, Maira de S.N., Castro, Alessandra M.M.G. de, Lima, Michele dos S., Pinto, Vivian da S.C., Silva, Thaís G. da, Fava, Claudia Del, Depes, Claudio Regis, Okuda, Liria H., Pituco, Edviges M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Veterinary Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470442/
https://www.ncbi.nlm.nih.gov/pubmed/28081979
http://dx.doi.org/10.1016/j.bjm.2016.10.021
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author Martins, Maira de S.N.
Castro, Alessandra M.M.G. de
Lima, Michele dos S.
Pinto, Vivian da S.C.
Silva, Thaís G. da
Fava, Claudia Del
Depes, Claudio Regis
Okuda, Liria H.
Pituco, Edviges M.
author_facet Martins, Maira de S.N.
Castro, Alessandra M.M.G. de
Lima, Michele dos S.
Pinto, Vivian da S.C.
Silva, Thaís G. da
Fava, Claudia Del
Depes, Claudio Regis
Okuda, Liria H.
Pituco, Edviges M.
author_sort Martins, Maira de S.N.
collection PubMed
description Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 10(1) DNA copies/μL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 10(0) DNA copy/μL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.
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spelling pubmed-54704422017-06-23 Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome Martins, Maira de S.N. Castro, Alessandra M.M.G. de Lima, Michele dos S. Pinto, Vivian da S.C. Silva, Thaís G. da Fava, Claudia Del Depes, Claudio Regis Okuda, Liria H. Pituco, Edviges M. Braz J Microbiol Veterinary Microbiology Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 10(1) DNA copies/μL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 10(0) DNA copy/μL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies. Elsevier 2017-01-02 /pmc/articles/PMC5470442/ /pubmed/28081979 http://dx.doi.org/10.1016/j.bjm.2016.10.021 Text en © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Veterinary Microbiology
Martins, Maira de S.N.
Castro, Alessandra M.M.G. de
Lima, Michele dos S.
Pinto, Vivian da S.C.
Silva, Thaís G. da
Fava, Claudia Del
Depes, Claudio Regis
Okuda, Liria H.
Pituco, Edviges M.
Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
title Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
title_full Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
title_fullStr Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
title_full_unstemmed Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
title_short Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome
title_sort malignant catarrhal fever in brazilian cattle presenting with neurological syndrome
topic Veterinary Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470442/
https://www.ncbi.nlm.nih.gov/pubmed/28081979
http://dx.doi.org/10.1016/j.bjm.2016.10.021
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