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Production of recombinant human epidermal growth factor in Pichia pastoris

This study was carried out to express human epidermal growth factor (hEGF) in Pichia pastoris GS115. For this aim, the hEGF gene was cloned into the pPIC9K expression vector, and then integrated into P. pastoris by electroporation. ELISA-based assay showed that the amount of hEGF secreted into the m...

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Detalles Bibliográficos
Autores principales:	Eissazadeh, Samira, Moeini, Hassan, Dezfouli, Maryam Ghandizadeh, Heidary, Somayyeh, Nelofer, Rubina, Abdullah, Mohd Puad
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2017
Materias:	Biotechnology and Industrial Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470459/ https://www.ncbi.nlm.nih.gov/pubmed/27998673 http://dx.doi.org/10.1016/j.bjm.2016.10.017

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