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Quantitative Trait Locus Mapping of Salt Tolerance and Identification of Salt-Tolerant Genes in Brassica napus L

Salinity stress is one of typical abiotic stresses that seriously limit crop production. In this study, a genetic linkage map based on 532 molecular markers covering 1341.1 cM was constructed to identify the loci associated with salt tolerance in Brassica napus. Up to 45 quantitative trait loci (QTL...

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Autores principales: Lang, Lina, Xu, Aixia, Ding, Juan, Zhang, Yan, Zhao, Na, Tian, Zhengshu, Liu, Yaping, Wang, Yang, Liu, Xia, Liang, Fenghao, Zhang, Bingbing, Qin, Mengfan, Dalelhan, Jazira, Huang, Zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470526/
https://www.ncbi.nlm.nih.gov/pubmed/28659949
http://dx.doi.org/10.3389/fpls.2017.01000
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author Lang, Lina
Xu, Aixia
Ding, Juan
Zhang, Yan
Zhao, Na
Tian, Zhengshu
Liu, Yaping
Wang, Yang
Liu, Xia
Liang, Fenghao
Zhang, Bingbing
Qin, Mengfan
Dalelhan, Jazira
Huang, Zhen
author_facet Lang, Lina
Xu, Aixia
Ding, Juan
Zhang, Yan
Zhao, Na
Tian, Zhengshu
Liu, Yaping
Wang, Yang
Liu, Xia
Liang, Fenghao
Zhang, Bingbing
Qin, Mengfan
Dalelhan, Jazira
Huang, Zhen
author_sort Lang, Lina
collection PubMed
description Salinity stress is one of typical abiotic stresses that seriously limit crop production. In this study, a genetic linkage map based on 532 molecular markers covering 1341.1 cM was constructed to identify the loci associated with salt tolerance in Brassica napus. Up to 45 quantitative trait loci (QTLs) for 10 indicators were identified in the F(2:3) populations. These QTLs can account for 4.80–51.14% of the phenotypic variation. A major QTL, qSPAD5 on LG5 associated with chlorophyll can be detected in three replicates. Two intron polymorphic (IP) markers in this QTL region were developed successfully to narrow down the QTL location to a region of 390 kb. A salt tolerance related gene Bra003640 was primary identified as the candidate gene in this region. The full length of the candidate gene was 1,063 bp containing three exons and two introns in B. napus L. The open reading frame (ORF) is 867 bp and encodes 287 amino acids. Three amino acid differences (34, 54, and 83) in the conserved domain (B-box) were identified. RT-qPCR analysis showed that the gene expression had significant difference between the two parents. The study laid great foundation for salt tolerance related gene mapping and cloning in B. napus L.
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spelling pubmed-54705262017-06-28 Quantitative Trait Locus Mapping of Salt Tolerance and Identification of Salt-Tolerant Genes in Brassica napus L Lang, Lina Xu, Aixia Ding, Juan Zhang, Yan Zhao, Na Tian, Zhengshu Liu, Yaping Wang, Yang Liu, Xia Liang, Fenghao Zhang, Bingbing Qin, Mengfan Dalelhan, Jazira Huang, Zhen Front Plant Sci Plant Science Salinity stress is one of typical abiotic stresses that seriously limit crop production. In this study, a genetic linkage map based on 532 molecular markers covering 1341.1 cM was constructed to identify the loci associated with salt tolerance in Brassica napus. Up to 45 quantitative trait loci (QTLs) for 10 indicators were identified in the F(2:3) populations. These QTLs can account for 4.80–51.14% of the phenotypic variation. A major QTL, qSPAD5 on LG5 associated with chlorophyll can be detected in three replicates. Two intron polymorphic (IP) markers in this QTL region were developed successfully to narrow down the QTL location to a region of 390 kb. A salt tolerance related gene Bra003640 was primary identified as the candidate gene in this region. The full length of the candidate gene was 1,063 bp containing three exons and two introns in B. napus L. The open reading frame (ORF) is 867 bp and encodes 287 amino acids. Three amino acid differences (34, 54, and 83) in the conserved domain (B-box) were identified. RT-qPCR analysis showed that the gene expression had significant difference between the two parents. The study laid great foundation for salt tolerance related gene mapping and cloning in B. napus L. Frontiers Media S.A. 2017-06-14 /pmc/articles/PMC5470526/ /pubmed/28659949 http://dx.doi.org/10.3389/fpls.2017.01000 Text en Copyright © 2017 Lang, Xu, Ding, Zhang, Zhao, Tian, Liu, Wang, Liu, Liang, Zhang, Qin, Dalelhan and Huang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lang, Lina
Xu, Aixia
Ding, Juan
Zhang, Yan
Zhao, Na
Tian, Zhengshu
Liu, Yaping
Wang, Yang
Liu, Xia
Liang, Fenghao
Zhang, Bingbing
Qin, Mengfan
Dalelhan, Jazira
Huang, Zhen
Quantitative Trait Locus Mapping of Salt Tolerance and Identification of Salt-Tolerant Genes in Brassica napus L
title Quantitative Trait Locus Mapping of Salt Tolerance and Identification of Salt-Tolerant Genes in Brassica napus L
title_full Quantitative Trait Locus Mapping of Salt Tolerance and Identification of Salt-Tolerant Genes in Brassica napus L
title_fullStr Quantitative Trait Locus Mapping of Salt Tolerance and Identification of Salt-Tolerant Genes in Brassica napus L
title_full_unstemmed Quantitative Trait Locus Mapping of Salt Tolerance and Identification of Salt-Tolerant Genes in Brassica napus L
title_short Quantitative Trait Locus Mapping of Salt Tolerance and Identification of Salt-Tolerant Genes in Brassica napus L
title_sort quantitative trait locus mapping of salt tolerance and identification of salt-tolerant genes in brassica napus l
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470526/
https://www.ncbi.nlm.nih.gov/pubmed/28659949
http://dx.doi.org/10.3389/fpls.2017.01000
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