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Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa)
Frizzled 6 (FZD6) encodes an integral membrane protein that functions in multiple signal transduction pathways, for example, as a receptor in Wnt/planar cell polarity (PCP) signaling pathway for polarized cell migration and organ morphogenesis. Mutations in FZD6 have been identified in a variety of...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470702/ https://www.ncbi.nlm.nih.gov/pubmed/28614361 http://dx.doi.org/10.1371/journal.pone.0179421 |
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author | Zou, Lijun Wang, Xiaocheng Jiang, Liping Wang, Shengping Xiong, Xia Yang, Huansheng Gao, Wei Gong, Min Hu, Chien-An A. Yin, Yulong |
author_facet | Zou, Lijun Wang, Xiaocheng Jiang, Liping Wang, Shengping Xiong, Xia Yang, Huansheng Gao, Wei Gong, Min Hu, Chien-An A. Yin, Yulong |
author_sort | Zou, Lijun |
collection | PubMed |
description | Frizzled 6 (FZD6) encodes an integral membrane protein that functions in multiple signal transduction pathways, for example, as a receptor in Wnt/planar cell polarity (PCP) signaling pathway for polarized cell migration and organ morphogenesis. Mutations in FZD6 have been identified in a variety of tumors. In this study, the full-length cDNA of Sus scrofa FZD6 (Sfz6) was cloned and characterized. Nucleotide sequence analysis demonstrates that the Sfz6 gene encodes the 712 amino-acid (aa) protein with seven transmembrane domain. Tissue distribution analysis showed that Sfz6 mRNA is ubiquitously expressed in various tissues, being highest in kidney, moderate in jejunum, ileum, colon, liver, and spleen. However, FZD6 protein is highly expressed in the heart and there was no significant difference in other tissues. The relative abundance and localization of FZD6 protein in jejunum along the crypt-villus axis was determined by Western blot and immunohistochemical localization. The results show that in the jejunum, FZD6 protein is highly expressed in the villus and less in the crypt cells. Cellular proliferation and viability assays indicate that knockdown of FZD6 with small interfering RNAs (siRNA) significantly reduced the cell viability of the intestinal porcine enterocyte cells (IPEC-J2). Furthermore, qPCR and Western blot analysis revealed that expressions of ras-related C3 botulinum toxin substrate 1 (Rac1); ras homolog gene family member A (RhoA) and c-Jun N-terminal kinase 1 (JNK1), some components of PCP signaling pathway were upregulated (P < 0.05) by knockdown of FZD6 in IPEC-J2 cells. In conclusion, these results showed that FZD6 abundance in the villus was higher than that in crypt cells and knockdown of FZD6 induces PCP signal pathway components expression in IPEC-J2 cells. Our findings provide the foundation for further investigation into porcine FZD6 gene. |
format | Online Article Text |
id | pubmed-5470702 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54707022017-07-03 Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa) Zou, Lijun Wang, Xiaocheng Jiang, Liping Wang, Shengping Xiong, Xia Yang, Huansheng Gao, Wei Gong, Min Hu, Chien-An A. Yin, Yulong PLoS One Research Article Frizzled 6 (FZD6) encodes an integral membrane protein that functions in multiple signal transduction pathways, for example, as a receptor in Wnt/planar cell polarity (PCP) signaling pathway for polarized cell migration and organ morphogenesis. Mutations in FZD6 have been identified in a variety of tumors. In this study, the full-length cDNA of Sus scrofa FZD6 (Sfz6) was cloned and characterized. Nucleotide sequence analysis demonstrates that the Sfz6 gene encodes the 712 amino-acid (aa) protein with seven transmembrane domain. Tissue distribution analysis showed that Sfz6 mRNA is ubiquitously expressed in various tissues, being highest in kidney, moderate in jejunum, ileum, colon, liver, and spleen. However, FZD6 protein is highly expressed in the heart and there was no significant difference in other tissues. The relative abundance and localization of FZD6 protein in jejunum along the crypt-villus axis was determined by Western blot and immunohistochemical localization. The results show that in the jejunum, FZD6 protein is highly expressed in the villus and less in the crypt cells. Cellular proliferation and viability assays indicate that knockdown of FZD6 with small interfering RNAs (siRNA) significantly reduced the cell viability of the intestinal porcine enterocyte cells (IPEC-J2). Furthermore, qPCR and Western blot analysis revealed that expressions of ras-related C3 botulinum toxin substrate 1 (Rac1); ras homolog gene family member A (RhoA) and c-Jun N-terminal kinase 1 (JNK1), some components of PCP signaling pathway were upregulated (P < 0.05) by knockdown of FZD6 in IPEC-J2 cells. In conclusion, these results showed that FZD6 abundance in the villus was higher than that in crypt cells and knockdown of FZD6 induces PCP signal pathway components expression in IPEC-J2 cells. Our findings provide the foundation for further investigation into porcine FZD6 gene. Public Library of Science 2017-06-14 /pmc/articles/PMC5470702/ /pubmed/28614361 http://dx.doi.org/10.1371/journal.pone.0179421 Text en © 2017 Zou et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zou, Lijun Wang, Xiaocheng Jiang, Liping Wang, Shengping Xiong, Xia Yang, Huansheng Gao, Wei Gong, Min Hu, Chien-An A. Yin, Yulong Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa) |
title | Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa) |
title_full | Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa) |
title_fullStr | Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa) |
title_full_unstemmed | Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa) |
title_short | Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa) |
title_sort | molecular cloning, characterization and expression analysis of frizzled 6 in the small intestine of pigs (sus scrofa) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470702/ https://www.ncbi.nlm.nih.gov/pubmed/28614361 http://dx.doi.org/10.1371/journal.pone.0179421 |
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