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Mechanical deformation induces depolarization of neutrophils
The transition of neutrophils from a resting state to a primed state is an essential requirement for their function as competent immune cells. This transition can be caused not only by chemical signals but also by mechanical perturbation. After cessation of either, these cells gradually revert to a...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470826/ https://www.ncbi.nlm.nih.gov/pubmed/28630905 http://dx.doi.org/10.1126/sciadv.1602536 |
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author | Ekpenyong, Andrew E. Toepfner, Nicole Fiddler, Christine Herbig, Maik Li, Wenhong Cojoc, Gheorghe Summers, Charlotte Guck, Jochen Chilvers, Edwin R. |
author_facet | Ekpenyong, Andrew E. Toepfner, Nicole Fiddler, Christine Herbig, Maik Li, Wenhong Cojoc, Gheorghe Summers, Charlotte Guck, Jochen Chilvers, Edwin R. |
author_sort | Ekpenyong, Andrew E. |
collection | PubMed |
description | The transition of neutrophils from a resting state to a primed state is an essential requirement for their function as competent immune cells. This transition can be caused not only by chemical signals but also by mechanical perturbation. After cessation of either, these cells gradually revert to a quiescent state over 40 to 120 min. We use two biophysical tools, an optical stretcher and a novel microcirculation mimetic, to effect physiologically relevant mechanical deformations of single nonadherent human neutrophils. We establish quantitative morphological analysis and mechanical phenotyping as label-free markers of neutrophil priming. We show that continued mechanical deformation of primed cells can cause active depolarization, which occurs two orders of magnitude faster than by spontaneous depriming. This work provides a cellular-level mechanism that potentially explains recent clinical studies demonstrating the potential importance, and physiological role, of neutrophil depriming in vivo and the pathophysiological implications when this deactivation is impaired, especially in disorders such as acute lung injury. |
format | Online Article Text |
id | pubmed-5470826 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54708262017-06-19 Mechanical deformation induces depolarization of neutrophils Ekpenyong, Andrew E. Toepfner, Nicole Fiddler, Christine Herbig, Maik Li, Wenhong Cojoc, Gheorghe Summers, Charlotte Guck, Jochen Chilvers, Edwin R. Sci Adv Research Articles The transition of neutrophils from a resting state to a primed state is an essential requirement for their function as competent immune cells. This transition can be caused not only by chemical signals but also by mechanical perturbation. After cessation of either, these cells gradually revert to a quiescent state over 40 to 120 min. We use two biophysical tools, an optical stretcher and a novel microcirculation mimetic, to effect physiologically relevant mechanical deformations of single nonadherent human neutrophils. We establish quantitative morphological analysis and mechanical phenotyping as label-free markers of neutrophil priming. We show that continued mechanical deformation of primed cells can cause active depolarization, which occurs two orders of magnitude faster than by spontaneous depriming. This work provides a cellular-level mechanism that potentially explains recent clinical studies demonstrating the potential importance, and physiological role, of neutrophil depriming in vivo and the pathophysiological implications when this deactivation is impaired, especially in disorders such as acute lung injury. American Association for the Advancement of Science 2017-06-14 /pmc/articles/PMC5470826/ /pubmed/28630905 http://dx.doi.org/10.1126/sciadv.1602536 Text en Copyright © 2017, The Authors http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Ekpenyong, Andrew E. Toepfner, Nicole Fiddler, Christine Herbig, Maik Li, Wenhong Cojoc, Gheorghe Summers, Charlotte Guck, Jochen Chilvers, Edwin R. Mechanical deformation induces depolarization of neutrophils |
title | Mechanical deformation induces depolarization of neutrophils |
title_full | Mechanical deformation induces depolarization of neutrophils |
title_fullStr | Mechanical deformation induces depolarization of neutrophils |
title_full_unstemmed | Mechanical deformation induces depolarization of neutrophils |
title_short | Mechanical deformation induces depolarization of neutrophils |
title_sort | mechanical deformation induces depolarization of neutrophils |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470826/ https://www.ncbi.nlm.nih.gov/pubmed/28630905 http://dx.doi.org/10.1126/sciadv.1602536 |
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