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Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis

A novel compact optical biochip based on a thin layer-sensing surface of nitrocellulose is used for in-situ label-free detection of metalloproteinase (MMP9) related to dry eye disease. In this article, a new integrated chip with different interferometric transducers layout with an optimal sensing su...

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Autores principales: Santamaría, Beatriz, Laguna, María F., López-Romero, David, Hernandez, Ana L., Sanza, Francisco J., Lavín, Álvaro, Casquel, Rafael, Maigler, María V., Espinosa, Rocío L., Holgado, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470904/
https://www.ncbi.nlm.nih.gov/pubmed/28534808
http://dx.doi.org/10.3390/s17051158
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author Santamaría, Beatriz
Laguna, María F.
López-Romero, David
Hernandez, Ana L.
Sanza, Francisco J.
Lavín, Álvaro
Casquel, Rafael
Maigler, María V.
Espinosa, Rocío L.
Holgado, Miguel
author_facet Santamaría, Beatriz
Laguna, María F.
López-Romero, David
Hernandez, Ana L.
Sanza, Francisco J.
Lavín, Álvaro
Casquel, Rafael
Maigler, María V.
Espinosa, Rocío L.
Holgado, Miguel
author_sort Santamaría, Beatriz
collection PubMed
description A novel compact optical biochip based on a thin layer-sensing surface of nitrocellulose is used for in-situ label-free detection of metalloproteinase (MMP9) related to dry eye disease. In this article, a new integrated chip with different interferometric transducers layout with an optimal sensing surface is reported for the first time. We demonstrate that specific antibodies can be immobilized onto these transducers with a very low volume of sample and with good orientation. Many sensing transducers constitute the presented biochip in order to yield statistical data and stability in the acquired measurements. As a result, we report the recognition curve for pure recombinant MMP9, tests of model tears with MMP9, and real tear performance from patients, with a promising limit of detection.
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spelling pubmed-54709042017-06-16 Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis Santamaría, Beatriz Laguna, María F. López-Romero, David Hernandez, Ana L. Sanza, Francisco J. Lavín, Álvaro Casquel, Rafael Maigler, María V. Espinosa, Rocío L. Holgado, Miguel Sensors (Basel) Article A novel compact optical biochip based on a thin layer-sensing surface of nitrocellulose is used for in-situ label-free detection of metalloproteinase (MMP9) related to dry eye disease. In this article, a new integrated chip with different interferometric transducers layout with an optimal sensing surface is reported for the first time. We demonstrate that specific antibodies can be immobilized onto these transducers with a very low volume of sample and with good orientation. Many sensing transducers constitute the presented biochip in order to yield statistical data and stability in the acquired measurements. As a result, we report the recognition curve for pure recombinant MMP9, tests of model tears with MMP9, and real tear performance from patients, with a promising limit of detection. MDPI 2017-05-19 /pmc/articles/PMC5470904/ /pubmed/28534808 http://dx.doi.org/10.3390/s17051158 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Santamaría, Beatriz
Laguna, María F.
López-Romero, David
Hernandez, Ana L.
Sanza, Francisco J.
Lavín, Álvaro
Casquel, Rafael
Maigler, María V.
Espinosa, Rocío L.
Holgado, Miguel
Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis
title Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis
title_full Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis
title_fullStr Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis
title_full_unstemmed Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis
title_short Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis
title_sort development towards compact nitrocellulose-based interferometric biochips for dry eye mmp9 label-free in-situ diagnosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470904/
https://www.ncbi.nlm.nih.gov/pubmed/28534808
http://dx.doi.org/10.3390/s17051158
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