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Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis
A novel compact optical biochip based on a thin layer-sensing surface of nitrocellulose is used for in-situ label-free detection of metalloproteinase (MMP9) related to dry eye disease. In this article, a new integrated chip with different interferometric transducers layout with an optimal sensing su...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470904/ https://www.ncbi.nlm.nih.gov/pubmed/28534808 http://dx.doi.org/10.3390/s17051158 |
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author | Santamaría, Beatriz Laguna, María F. López-Romero, David Hernandez, Ana L. Sanza, Francisco J. Lavín, Álvaro Casquel, Rafael Maigler, María V. Espinosa, Rocío L. Holgado, Miguel |
author_facet | Santamaría, Beatriz Laguna, María F. López-Romero, David Hernandez, Ana L. Sanza, Francisco J. Lavín, Álvaro Casquel, Rafael Maigler, María V. Espinosa, Rocío L. Holgado, Miguel |
author_sort | Santamaría, Beatriz |
collection | PubMed |
description | A novel compact optical biochip based on a thin layer-sensing surface of nitrocellulose is used for in-situ label-free detection of metalloproteinase (MMP9) related to dry eye disease. In this article, a new integrated chip with different interferometric transducers layout with an optimal sensing surface is reported for the first time. We demonstrate that specific antibodies can be immobilized onto these transducers with a very low volume of sample and with good orientation. Many sensing transducers constitute the presented biochip in order to yield statistical data and stability in the acquired measurements. As a result, we report the recognition curve for pure recombinant MMP9, tests of model tears with MMP9, and real tear performance from patients, with a promising limit of detection. |
format | Online Article Text |
id | pubmed-5470904 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54709042017-06-16 Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis Santamaría, Beatriz Laguna, María F. López-Romero, David Hernandez, Ana L. Sanza, Francisco J. Lavín, Álvaro Casquel, Rafael Maigler, María V. Espinosa, Rocío L. Holgado, Miguel Sensors (Basel) Article A novel compact optical biochip based on a thin layer-sensing surface of nitrocellulose is used for in-situ label-free detection of metalloproteinase (MMP9) related to dry eye disease. In this article, a new integrated chip with different interferometric transducers layout with an optimal sensing surface is reported for the first time. We demonstrate that specific antibodies can be immobilized onto these transducers with a very low volume of sample and with good orientation. Many sensing transducers constitute the presented biochip in order to yield statistical data and stability in the acquired measurements. As a result, we report the recognition curve for pure recombinant MMP9, tests of model tears with MMP9, and real tear performance from patients, with a promising limit of detection. MDPI 2017-05-19 /pmc/articles/PMC5470904/ /pubmed/28534808 http://dx.doi.org/10.3390/s17051158 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Santamaría, Beatriz Laguna, María F. López-Romero, David Hernandez, Ana L. Sanza, Francisco J. Lavín, Álvaro Casquel, Rafael Maigler, María V. Espinosa, Rocío L. Holgado, Miguel Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis |
title | Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis |
title_full | Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis |
title_fullStr | Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis |
title_full_unstemmed | Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis |
title_short | Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis |
title_sort | development towards compact nitrocellulose-based interferometric biochips for dry eye mmp9 label-free in-situ diagnosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470904/ https://www.ncbi.nlm.nih.gov/pubmed/28534808 http://dx.doi.org/10.3390/s17051158 |
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