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Reconstruction of pathway modification induced by nicotinamide using multi-omic network analyses in triple negative breast cancer
Triple negative breast cancer (TNBC) is characterized by an aggressive biological behavior in the absence of a specific target agent. Nicotinamide has recently been proven to be a novel therapeutic agent for skin tumors in an ONTRAC trial. We performed combinatory transcriptomic and in-depth proteom...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5471278/ https://www.ncbi.nlm.nih.gov/pubmed/28615672 http://dx.doi.org/10.1038/s41598-017-03322-7 |
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author | Kim, Ji Young Lee, Hyebin Woo, Jongmin Yue, Wang Kim, Kwangsoo Choi, Seongmin Jang, Ja-June Kim, Youngsoo Park, In Ae Han, Dohyun Ryu, Han Suk |
author_facet | Kim, Ji Young Lee, Hyebin Woo, Jongmin Yue, Wang Kim, Kwangsoo Choi, Seongmin Jang, Ja-June Kim, Youngsoo Park, In Ae Han, Dohyun Ryu, Han Suk |
author_sort | Kim, Ji Young |
collection | PubMed |
description | Triple negative breast cancer (TNBC) is characterized by an aggressive biological behavior in the absence of a specific target agent. Nicotinamide has recently been proven to be a novel therapeutic agent for skin tumors in an ONTRAC trial. We performed combinatory transcriptomic and in-depth proteomic analyses to characterize the network of molecular interactions in TNBC cells treated with nicotinamide. The multi-omic profiles revealed that nicotinamide drives significant functional alterations related to major cellular pathways, including the cell cycle, DNA replication, apoptosis and DNA damage repair. We further elaborated the global interaction networks of molecular events via nicotinamide-inducible expression changes at the mRNA and functional protein levels. This approach indicated that nicotinamide treatment rewires interaction networks toward dysfunction in DNA damage repair and away from a pro-growth state in TNBC. To our knowledge, the high-resolution network interactions identified in the present study provide the first evidence to comprehensively support the hypothesis of nicotinamide as a novel therapeutic agent in TNBC. |
format | Online Article Text |
id | pubmed-5471278 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54712782017-06-19 Reconstruction of pathway modification induced by nicotinamide using multi-omic network analyses in triple negative breast cancer Kim, Ji Young Lee, Hyebin Woo, Jongmin Yue, Wang Kim, Kwangsoo Choi, Seongmin Jang, Ja-June Kim, Youngsoo Park, In Ae Han, Dohyun Ryu, Han Suk Sci Rep Article Triple negative breast cancer (TNBC) is characterized by an aggressive biological behavior in the absence of a specific target agent. Nicotinamide has recently been proven to be a novel therapeutic agent for skin tumors in an ONTRAC trial. We performed combinatory transcriptomic and in-depth proteomic analyses to characterize the network of molecular interactions in TNBC cells treated with nicotinamide. The multi-omic profiles revealed that nicotinamide drives significant functional alterations related to major cellular pathways, including the cell cycle, DNA replication, apoptosis and DNA damage repair. We further elaborated the global interaction networks of molecular events via nicotinamide-inducible expression changes at the mRNA and functional protein levels. This approach indicated that nicotinamide treatment rewires interaction networks toward dysfunction in DNA damage repair and away from a pro-growth state in TNBC. To our knowledge, the high-resolution network interactions identified in the present study provide the first evidence to comprehensively support the hypothesis of nicotinamide as a novel therapeutic agent in TNBC. Nature Publishing Group UK 2017-06-14 /pmc/articles/PMC5471278/ /pubmed/28615672 http://dx.doi.org/10.1038/s41598-017-03322-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kim, Ji Young Lee, Hyebin Woo, Jongmin Yue, Wang Kim, Kwangsoo Choi, Seongmin Jang, Ja-June Kim, Youngsoo Park, In Ae Han, Dohyun Ryu, Han Suk Reconstruction of pathway modification induced by nicotinamide using multi-omic network analyses in triple negative breast cancer |
title | Reconstruction of pathway modification induced by nicotinamide using multi-omic network analyses in triple negative breast cancer |
title_full | Reconstruction of pathway modification induced by nicotinamide using multi-omic network analyses in triple negative breast cancer |
title_fullStr | Reconstruction of pathway modification induced by nicotinamide using multi-omic network analyses in triple negative breast cancer |
title_full_unstemmed | Reconstruction of pathway modification induced by nicotinamide using multi-omic network analyses in triple negative breast cancer |
title_short | Reconstruction of pathway modification induced by nicotinamide using multi-omic network analyses in triple negative breast cancer |
title_sort | reconstruction of pathway modification induced by nicotinamide using multi-omic network analyses in triple negative breast cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5471278/ https://www.ncbi.nlm.nih.gov/pubmed/28615672 http://dx.doi.org/10.1038/s41598-017-03322-7 |
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