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INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO
BACKGROUND: Chitosan oligosaccharide, the degradation products of chitin, was reported to have a wide range of physiological functions and biological activities. In this study, we explored the inhibitory effect of Chitosan oligosaccharide on human hepatoma cells MATERIALS AND METHODS: MTT assay was...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
African Traditional Herbal Medicine Supporters Initiative (ATHMSI)
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5471475/ https://www.ncbi.nlm.nih.gov/pubmed/28638890 http://dx.doi.org/10.21010/ajtcam.v14i4.30 |
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author | Liu, Likun Xin, Yi Liu, Jia Zhang, Ershao Li, Weiling |
author_facet | Liu, Likun Xin, Yi Liu, Jia Zhang, Ershao Li, Weiling |
author_sort | Liu, Likun |
collection | PubMed |
description | BACKGROUND: Chitosan oligosaccharide, the degradation products of chitin, was reported to have a wide range of physiological functions and biological activities. In this study, we explored the inhibitory effect of Chitosan oligosaccharide on human hepatoma cells MATERIALS AND METHODS: MTT assay was applied to detect cell viability of the human hepatoma cells treated with Chitosan oligosaccharide. Flow cytometric analysis was used to investigate the apoptosis of the human hepatoma cells treated with Chitosan oligosaccharide. We employed western blot to investigate the underlying mechanisms involved in the apoptosis. RESULTS: Our data indicated that chitosan oligosaccharide dose-dependently inhibited the growth of hepatoma cells and induced apoptosis. On the molecular level, chitosan oligosaccharide decreased Bcl-2 and increased Caspase-3 expression which may be related to the apoptosis of hepatoma cells. CONCLUSION: Our results provide an experimental basis for the clinical development of Chitosan oligosaccharide as a novel anti-hepatoma drug. |
format | Online Article Text |
id | pubmed-5471475 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | African Traditional Herbal Medicine Supporters Initiative (ATHMSI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-54714752017-06-21 INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO Liu, Likun Xin, Yi Liu, Jia Zhang, Ershao Li, Weiling Afr J Tradit Complement Altern Med Article BACKGROUND: Chitosan oligosaccharide, the degradation products of chitin, was reported to have a wide range of physiological functions and biological activities. In this study, we explored the inhibitory effect of Chitosan oligosaccharide on human hepatoma cells MATERIALS AND METHODS: MTT assay was applied to detect cell viability of the human hepatoma cells treated with Chitosan oligosaccharide. Flow cytometric analysis was used to investigate the apoptosis of the human hepatoma cells treated with Chitosan oligosaccharide. We employed western blot to investigate the underlying mechanisms involved in the apoptosis. RESULTS: Our data indicated that chitosan oligosaccharide dose-dependently inhibited the growth of hepatoma cells and induced apoptosis. On the molecular level, chitosan oligosaccharide decreased Bcl-2 and increased Caspase-3 expression which may be related to the apoptosis of hepatoma cells. CONCLUSION: Our results provide an experimental basis for the clinical development of Chitosan oligosaccharide as a novel anti-hepatoma drug. African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2017-06-05 /pmc/articles/PMC5471475/ /pubmed/28638890 http://dx.doi.org/10.21010/ajtcam.v14i4.30 Text en Copyright: © 2017 Afr. J. Traditional Complementary and Alternative Medicines http://creativecommons.org/licenses/CC-BY/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License |
spellingShingle | Article Liu, Likun Xin, Yi Liu, Jia Zhang, Ershao Li, Weiling INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO |
title | INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO |
title_full | INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO |
title_fullStr | INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO |
title_full_unstemmed | INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO |
title_short | INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO |
title_sort | inhibitory effect of chitosan oligosaccharide on human hepatoma cells in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5471475/ https://www.ncbi.nlm.nih.gov/pubmed/28638890 http://dx.doi.org/10.21010/ajtcam.v14i4.30 |
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