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INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO

BACKGROUND: Chitosan oligosaccharide, the degradation products of chitin, was reported to have a wide range of physiological functions and biological activities. In this study, we explored the inhibitory effect of Chitosan oligosaccharide on human hepatoma cells MATERIALS AND METHODS: MTT assay was...

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Detalles Bibliográficos
Autores principales: Liu, Likun, Xin, Yi, Liu, Jia, Zhang, Ershao, Li, Weiling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5471475/
https://www.ncbi.nlm.nih.gov/pubmed/28638890
http://dx.doi.org/10.21010/ajtcam.v14i4.30
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author Liu, Likun
Xin, Yi
Liu, Jia
Zhang, Ershao
Li, Weiling
author_facet Liu, Likun
Xin, Yi
Liu, Jia
Zhang, Ershao
Li, Weiling
author_sort Liu, Likun
collection PubMed
description BACKGROUND: Chitosan oligosaccharide, the degradation products of chitin, was reported to have a wide range of physiological functions and biological activities. In this study, we explored the inhibitory effect of Chitosan oligosaccharide on human hepatoma cells MATERIALS AND METHODS: MTT assay was applied to detect cell viability of the human hepatoma cells treated with Chitosan oligosaccharide. Flow cytometric analysis was used to investigate the apoptosis of the human hepatoma cells treated with Chitosan oligosaccharide. We employed western blot to investigate the underlying mechanisms involved in the apoptosis. RESULTS: Our data indicated that chitosan oligosaccharide dose-dependently inhibited the growth of hepatoma cells and induced apoptosis. On the molecular level, chitosan oligosaccharide decreased Bcl-2 and increased Caspase-3 expression which may be related to the apoptosis of hepatoma cells. CONCLUSION: Our results provide an experimental basis for the clinical development of Chitosan oligosaccharide as a novel anti-hepatoma drug.
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spelling pubmed-54714752017-06-21 INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO Liu, Likun Xin, Yi Liu, Jia Zhang, Ershao Li, Weiling Afr J Tradit Complement Altern Med Article BACKGROUND: Chitosan oligosaccharide, the degradation products of chitin, was reported to have a wide range of physiological functions and biological activities. In this study, we explored the inhibitory effect of Chitosan oligosaccharide on human hepatoma cells MATERIALS AND METHODS: MTT assay was applied to detect cell viability of the human hepatoma cells treated with Chitosan oligosaccharide. Flow cytometric analysis was used to investigate the apoptosis of the human hepatoma cells treated with Chitosan oligosaccharide. We employed western blot to investigate the underlying mechanisms involved in the apoptosis. RESULTS: Our data indicated that chitosan oligosaccharide dose-dependently inhibited the growth of hepatoma cells and induced apoptosis. On the molecular level, chitosan oligosaccharide decreased Bcl-2 and increased Caspase-3 expression which may be related to the apoptosis of hepatoma cells. CONCLUSION: Our results provide an experimental basis for the clinical development of Chitosan oligosaccharide as a novel anti-hepatoma drug. African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2017-06-05 /pmc/articles/PMC5471475/ /pubmed/28638890 http://dx.doi.org/10.21010/ajtcam.v14i4.30 Text en Copyright: © 2017 Afr. J. Traditional Complementary and Alternative Medicines http://creativecommons.org/licenses/CC-BY/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
spellingShingle Article
Liu, Likun
Xin, Yi
Liu, Jia
Zhang, Ershao
Li, Weiling
INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO
title INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO
title_full INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO
title_fullStr INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO
title_full_unstemmed INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO
title_short INHIBITORY EFFECT OF CHITOSAN OLIGOSACCHARIDE ON HUMAN HEPATOMA CELLS IN VITRO
title_sort inhibitory effect of chitosan oligosaccharide on human hepatoma cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5471475/
https://www.ncbi.nlm.nih.gov/pubmed/28638890
http://dx.doi.org/10.21010/ajtcam.v14i4.30
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