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Direct Estimation of Reference Intervals for Thyroid Parameters in the Republic of Srpska

BACKGROUND: The aim of this study was to determine the reference values for thyrotropin (TSH), thyroid hormones (total and free thyroxine, T4 and fT4; total and free triiodothyronine, T3 and fT3), thyroglobulin (Tg) and thyroid antibodies (thyroid peroxidase, TPOAb and thyroglobulin antibody, TgAb)...

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Autores principales: Mirjanic-Azaric, Bosa, Avram, Sanja, Stojakovic-Jelisavac, Tanja, Stojanovic, Darja, Petkovic, Mira, Bogavac-Stanojevic, Natasa, Ignjatovic, Svetlana, Stojanov, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Medical Biochemists of Serbia 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5471646/
https://www.ncbi.nlm.nih.gov/pubmed/28680357
http://dx.doi.org/10.1515/jomb-2017-0008
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author Mirjanic-Azaric, Bosa
Avram, Sanja
Stojakovic-Jelisavac, Tanja
Stojanovic, Darja
Petkovic, Mira
Bogavac-Stanojevic, Natasa
Ignjatovic, Svetlana
Stojanov, Marina
author_facet Mirjanic-Azaric, Bosa
Avram, Sanja
Stojakovic-Jelisavac, Tanja
Stojanovic, Darja
Petkovic, Mira
Bogavac-Stanojevic, Natasa
Ignjatovic, Svetlana
Stojanov, Marina
author_sort Mirjanic-Azaric, Bosa
collection PubMed
description BACKGROUND: The aim of this study was to determine the reference values for thyrotropin (TSH), thyroid hormones (total and free thyroxine, T4 and fT4; total and free triiodothyronine, T3 and fT3), thyroglobulin (Tg) and thyroid antibodies (thyroid peroxidase, TPOAb and thyroglobulin antibody, TgAb) in the population of the Republic of Srpska. METHODS: A total of 250 euthyroid subjects were enrolled in this study. A direct method for choosing reference subjects was used to establish reference intervals. The hormones and thyroid antibodies were measured by an electrochemiluminescence immunoassay method (ECLIA, Roche Diagnostics, Mannheim, Germany). We calculated the reference intervals by MedCalc, version 12.1.4.0 (MedCalc software, Belgium) as recommended by the IFCC (CLSI C28-A3). RESULTS: Using guidelines recommended by the National Academy of Clinical Biochemistry (NACB) and based on standard statistical approaches, the reference intervals derived for TSH, fT4, T4, fT3, T3 were 0.75–5.32 mIU/L, 12.29–20.03 pmol/L, 73.49–126,30 nmol/L, 4.11–6.32 pmol/L, 1.15–2.32 nmol/L and for Tg, TPOAb, TgAb were 3.63–26.00 μg/L, <18.02 mIU/L, < 98.00 mIU/L, respectively. We found a significant difference (p<0.05) in TSH and fT3 values between different age groups as well as in T4, fT4 and fT3 values between ge nder groups. CONCLUSIONS: The established reference values for the population of the Republic of Srpska were significantly different from the values recommended by the manufacturer of reagents (Roche Diagnostics). Our results showed that a laboratory needs to establish its own reference values in order to set up a proper diagnosis, as well as to treat patients successfully.
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spelling pubmed-54716462017-07-05 Direct Estimation of Reference Intervals for Thyroid Parameters in the Republic of Srpska Mirjanic-Azaric, Bosa Avram, Sanja Stojakovic-Jelisavac, Tanja Stojanovic, Darja Petkovic, Mira Bogavac-Stanojevic, Natasa Ignjatovic, Svetlana Stojanov, Marina J Med Biochem Original Paper BACKGROUND: The aim of this study was to determine the reference values for thyrotropin (TSH), thyroid hormones (total and free thyroxine, T4 and fT4; total and free triiodothyronine, T3 and fT3), thyroglobulin (Tg) and thyroid antibodies (thyroid peroxidase, TPOAb and thyroglobulin antibody, TgAb) in the population of the Republic of Srpska. METHODS: A total of 250 euthyroid subjects were enrolled in this study. A direct method for choosing reference subjects was used to establish reference intervals. The hormones and thyroid antibodies were measured by an electrochemiluminescence immunoassay method (ECLIA, Roche Diagnostics, Mannheim, Germany). We calculated the reference intervals by MedCalc, version 12.1.4.0 (MedCalc software, Belgium) as recommended by the IFCC (CLSI C28-A3). RESULTS: Using guidelines recommended by the National Academy of Clinical Biochemistry (NACB) and based on standard statistical approaches, the reference intervals derived for TSH, fT4, T4, fT3, T3 were 0.75–5.32 mIU/L, 12.29–20.03 pmol/L, 73.49–126,30 nmol/L, 4.11–6.32 pmol/L, 1.15–2.32 nmol/L and for Tg, TPOAb, TgAb were 3.63–26.00 μg/L, <18.02 mIU/L, < 98.00 mIU/L, respectively. We found a significant difference (p<0.05) in TSH and fT3 values between different age groups as well as in T4, fT4 and fT3 values between ge nder groups. CONCLUSIONS: The established reference values for the population of the Republic of Srpska were significantly different from the values recommended by the manufacturer of reagents (Roche Diagnostics). Our results showed that a laboratory needs to establish its own reference values in order to set up a proper diagnosis, as well as to treat patients successfully. Society of Medical Biochemists of Serbia 2017-04-22 /pmc/articles/PMC5471646/ /pubmed/28680357 http://dx.doi.org/10.1515/jomb-2017-0008 Text en © by Bosa Mirjanic-Azaric. http://creativecommons.org/licenses/by-nc-nd/4.0 This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
spellingShingle Original Paper
Mirjanic-Azaric, Bosa
Avram, Sanja
Stojakovic-Jelisavac, Tanja
Stojanovic, Darja
Petkovic, Mira
Bogavac-Stanojevic, Natasa
Ignjatovic, Svetlana
Stojanov, Marina
Direct Estimation of Reference Intervals for Thyroid Parameters in the Republic of Srpska
title Direct Estimation of Reference Intervals for Thyroid Parameters in the Republic of Srpska
title_full Direct Estimation of Reference Intervals for Thyroid Parameters in the Republic of Srpska
title_fullStr Direct Estimation of Reference Intervals for Thyroid Parameters in the Republic of Srpska
title_full_unstemmed Direct Estimation of Reference Intervals for Thyroid Parameters in the Republic of Srpska
title_short Direct Estimation of Reference Intervals for Thyroid Parameters in the Republic of Srpska
title_sort direct estimation of reference intervals for thyroid parameters in the republic of srpska
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5471646/
https://www.ncbi.nlm.nih.gov/pubmed/28680357
http://dx.doi.org/10.1515/jomb-2017-0008
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