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Efficient soluble expression of disulfide bonded proteins in the cytoplasm of Escherichia coli in fed-batch fermentations on chemically defined minimal media

BACKGROUND: The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in the cytoplasm in the form of inclusion bodies, then solubilized and re-folded...

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Detalles Bibliográficos
Autores principales:	Gąciarz, Anna, Khatri, Narendar Kumar, Velez-Suberbie, M. Lourdes, Saaranen, Mirva J., Uchida, Yuko, Keshavarz-Moore, Eli, Ruddock, Lloyd W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5471842/ https://www.ncbi.nlm.nih.gov/pubmed/28619018 http://dx.doi.org/10.1186/s12934-017-0721-x

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