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The Expression of STAT3 and STAT5A Genes in Severe Refractory Asthma
BACKGROUND: Despite being a high burden disorder, the pathogenesis of severe refractory asthma (SRA) is poorly understood. There are some evidences for the involvement of members of the signal transducer and activator of transcription (STAT) family, including STAT3 and STAT5a. Our study aimed to eva...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Research Institute of Tuberculosis and Lung Disease
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473377/ https://www.ncbi.nlm.nih.gov/pubmed/28638418 |
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author | Saeedfar, Kayvan Behmanesh, Mehrdad Mortaz, Esmaeil Masjedi, Mohammad Reza |
author_facet | Saeedfar, Kayvan Behmanesh, Mehrdad Mortaz, Esmaeil Masjedi, Mohammad Reza |
author_sort | Saeedfar, Kayvan |
collection | PubMed |
description | BACKGROUND: Despite being a high burden disorder, the pathogenesis of severe refractory asthma (SRA) is poorly understood. There are some evidences for the involvement of members of the signal transducer and activator of transcription (STAT) family, including STAT3 and STAT5a. Our study aimed to evaluate the gene expression of STAT3 and STAT5a in asthma and SRA to establish if there is an association. MATERIALS AND METHODS: Using quantitative real-time polymerase chain reactions (qRT-PCR), the transcript levels of STAT3 and STAT5a were evaluated in peripheral blood mononuclear lymphocytes (PBML) isolated from 13 patients with SRA, 14 with mild asthma, and 30 healthy volunteers. RESULTS: There were no significant differences in STAT3 transcript levels between study groups. There was however a significant difference in STAT5a transcript levels between cases and controls (p-value=0.03). In comparison to healthy controls, the levels of STAT5a were notably lower in patients with mild asthma and significantly least in those with SRA. CONCLUSION: Our study found no appreciable association between STAT3 gene expression and either mild asthma or SRA. However, the STAT5a down regulation in asthmatics and especially SRA is a notable finding which denotes on association between STAT5a and different level of asthma. |
format | Online Article Text |
id | pubmed-5473377 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | National Research Institute of Tuberculosis and Lung Disease |
record_format | MEDLINE/PubMed |
spelling | pubmed-54733772017-06-21 The Expression of STAT3 and STAT5A Genes in Severe Refractory Asthma Saeedfar, Kayvan Behmanesh, Mehrdad Mortaz, Esmaeil Masjedi, Mohammad Reza Tanaffos Original Article BACKGROUND: Despite being a high burden disorder, the pathogenesis of severe refractory asthma (SRA) is poorly understood. There are some evidences for the involvement of members of the signal transducer and activator of transcription (STAT) family, including STAT3 and STAT5a. Our study aimed to evaluate the gene expression of STAT3 and STAT5a in asthma and SRA to establish if there is an association. MATERIALS AND METHODS: Using quantitative real-time polymerase chain reactions (qRT-PCR), the transcript levels of STAT3 and STAT5a were evaluated in peripheral blood mononuclear lymphocytes (PBML) isolated from 13 patients with SRA, 14 with mild asthma, and 30 healthy volunteers. RESULTS: There were no significant differences in STAT3 transcript levels between study groups. There was however a significant difference in STAT5a transcript levels between cases and controls (p-value=0.03). In comparison to healthy controls, the levels of STAT5a were notably lower in patients with mild asthma and significantly least in those with SRA. CONCLUSION: Our study found no appreciable association between STAT3 gene expression and either mild asthma or SRA. However, the STAT5a down regulation in asthmatics and especially SRA is a notable finding which denotes on association between STAT5a and different level of asthma. National Research Institute of Tuberculosis and Lung Disease 2017 /pmc/articles/PMC5473377/ /pubmed/28638418 Text en Copyright© 2017 National Research Institute of Tuberculosis and Lung Disease http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Saeedfar, Kayvan Behmanesh, Mehrdad Mortaz, Esmaeil Masjedi, Mohammad Reza The Expression of STAT3 and STAT5A Genes in Severe Refractory Asthma |
title | The Expression of STAT3 and STAT5A Genes in Severe Refractory Asthma |
title_full | The Expression of STAT3 and STAT5A Genes in Severe Refractory Asthma |
title_fullStr | The Expression of STAT3 and STAT5A Genes in Severe Refractory Asthma |
title_full_unstemmed | The Expression of STAT3 and STAT5A Genes in Severe Refractory Asthma |
title_short | The Expression of STAT3 and STAT5A Genes in Severe Refractory Asthma |
title_sort | expression of stat3 and stat5a genes in severe refractory asthma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473377/ https://www.ncbi.nlm.nih.gov/pubmed/28638418 |
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