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In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery
The addition of an antibacterial agent to dental implants may provide the opportunity to decrease the percentage of implant failures due to peri-implantitis. For this purpose, in this study, the potential efficacy of nanosilver-doped titanium biomaterials was determined. Titanium disks were incorpor...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473602/ https://www.ncbi.nlm.nih.gov/pubmed/28652733 http://dx.doi.org/10.2147/IJN.S131163 |
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author | Pokrowiecki, Rafał Zaręba, Tomasz Szaraniec, Barbara Pałka, Krzysztof Mielczarek, Agnieszka Menaszek, Elżbieta Tyski, Stefan |
author_facet | Pokrowiecki, Rafał Zaręba, Tomasz Szaraniec, Barbara Pałka, Krzysztof Mielczarek, Agnieszka Menaszek, Elżbieta Tyski, Stefan |
author_sort | Pokrowiecki, Rafał |
collection | PubMed |
description | The addition of an antibacterial agent to dental implants may provide the opportunity to decrease the percentage of implant failures due to peri-implantitis. For this purpose, in this study, the potential efficacy of nanosilver-doped titanium biomaterials was determined. Titanium disks were incorporated with silver nanoparticles over different time periods by Tollens reaction, which is considered to be an eco-friendly, cheap, and easy-to-perform method. The surface roughness, wettability, and silver release profile of each disc were measured. In addition, the antibacterial activity was also evaluated by using disk diffusion tests for bacteria frequently isolated from the peri-implant biofilm: Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, Streptococcus sanguis, Porphyromonas gingivalis, Staphylococcus aureus, and Escherichia coli. Cytotoxicity was evaluated in vitro in a natural human osteoblasts cell culture. The addition of nanosilver significantly increased the surface roughness and decreased the wettability in a dose-dependent manner. These surfaces were significantly toxic to all the tested bacteria following a 48-hour exposure, regardless of silver doping duration. A concentration of 0.05 ppm was sufficient to inhibit Gram-positive and Gram-negative species, with the latter being significantly more susceptible to silver ions. However, after the exposure of human osteoblasts to 0.1 ppm of silver ions, a significant decrease in cell viability was observed by using ToxiLight™ BioAssay Kit after 72 hours. Data from the present study indicated that the incorporation of nanosilver may influence the surface properties that are important in the implant healing process. The presence of nanosilver on the titanium provides an antibacterial activity related to the bacteria involved in peri-implantitis. Finally, the potential toxicological considerations of nanosilver should further be investigated, as both the antibacterial and cytotoxic properties may be observed at similar concentration ranges. |
format | Online Article Text |
id | pubmed-5473602 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-54736022017-06-26 In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery Pokrowiecki, Rafał Zaręba, Tomasz Szaraniec, Barbara Pałka, Krzysztof Mielczarek, Agnieszka Menaszek, Elżbieta Tyski, Stefan Int J Nanomedicine Original Research The addition of an antibacterial agent to dental implants may provide the opportunity to decrease the percentage of implant failures due to peri-implantitis. For this purpose, in this study, the potential efficacy of nanosilver-doped titanium biomaterials was determined. Titanium disks were incorporated with silver nanoparticles over different time periods by Tollens reaction, which is considered to be an eco-friendly, cheap, and easy-to-perform method. The surface roughness, wettability, and silver release profile of each disc were measured. In addition, the antibacterial activity was also evaluated by using disk diffusion tests for bacteria frequently isolated from the peri-implant biofilm: Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, Streptococcus sanguis, Porphyromonas gingivalis, Staphylococcus aureus, and Escherichia coli. Cytotoxicity was evaluated in vitro in a natural human osteoblasts cell culture. The addition of nanosilver significantly increased the surface roughness and decreased the wettability in a dose-dependent manner. These surfaces were significantly toxic to all the tested bacteria following a 48-hour exposure, regardless of silver doping duration. A concentration of 0.05 ppm was sufficient to inhibit Gram-positive and Gram-negative species, with the latter being significantly more susceptible to silver ions. However, after the exposure of human osteoblasts to 0.1 ppm of silver ions, a significant decrease in cell viability was observed by using ToxiLight™ BioAssay Kit after 72 hours. Data from the present study indicated that the incorporation of nanosilver may influence the surface properties that are important in the implant healing process. The presence of nanosilver on the titanium provides an antibacterial activity related to the bacteria involved in peri-implantitis. Finally, the potential toxicological considerations of nanosilver should further be investigated, as both the antibacterial and cytotoxic properties may be observed at similar concentration ranges. Dove Medical Press 2017-06-06 /pmc/articles/PMC5473602/ /pubmed/28652733 http://dx.doi.org/10.2147/IJN.S131163 Text en © 2017 Pokrowiecki et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Pokrowiecki, Rafał Zaręba, Tomasz Szaraniec, Barbara Pałka, Krzysztof Mielczarek, Agnieszka Menaszek, Elżbieta Tyski, Stefan In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery |
title | In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery |
title_full | In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery |
title_fullStr | In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery |
title_full_unstemmed | In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery |
title_short | In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery |
title_sort | in vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473602/ https://www.ncbi.nlm.nih.gov/pubmed/28652733 http://dx.doi.org/10.2147/IJN.S131163 |
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