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Comparison of in vitro fertilization outcomes in ICSI cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation

OBJECTIVE: The aim of this study was to evaluate the efficacy of a non-expensive, easy and fast technique (direct micro swim-up) for sperm preparation in intracytoplasmic sperm injection (ICSI) treatments without the use of centrifuge. METHODS: We carried out a multicentric study in which a total of...

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Autores principales: Palini, Simone, Stefani, Silvia De, Primiterra, Mariangela, Benedetti, Serena, Barone, Stefano, Carli, Luca, Vaccari, Enrico, Murat, Ulug, Feichtinger, Wilfried
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Assisted Reproduction 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473700/
https://www.ncbi.nlm.nih.gov/pubmed/28609274
http://dx.doi.org/10.5935/1518-0557.20170022
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author Palini, Simone
Stefani, Silvia De
Primiterra, Mariangela
Benedetti, Serena
Barone, Stefano
Carli, Luca
Vaccari, Enrico
Murat, Ulug
Feichtinger, Wilfried
author_facet Palini, Simone
Stefani, Silvia De
Primiterra, Mariangela
Benedetti, Serena
Barone, Stefano
Carli, Luca
Vaccari, Enrico
Murat, Ulug
Feichtinger, Wilfried
author_sort Palini, Simone
collection PubMed
description OBJECTIVE: The aim of this study was to evaluate the efficacy of a non-expensive, easy and fast technique (direct micro swim-up) for sperm preparation in intracytoplasmic sperm injection (ICSI) treatments without the use of centrifuge. METHODS: We carried out a multicentric study in which a total of 140 ICSI-cycles were included. Sibling oocytes were divided into two groups according to semen preparation procedures: group A, discontinuous gradients (DG) (oocytes n=668), and group B, direct micro swim-up (MSU) (oocytes n=660). We analyzed differences in some key performance indicators. RESULTS: Fertilization rates were not statistically different between the DG and MSU groups (76.0% vs. 81.8%, respectively, p=0.248); while significant differences were found in blastulation rates per fertilized oocytes (41.7% vs. 58.5%, p=0.009), blastulation rates per D3 embryos (46.1% vs. 63.7%, p=0.045), and pregnancy rates (25.8% vs. 41.9%, p=0.045). The abortion rate was reduced in the MSU group as compared to DG, but not in a significant manner (12.9% vs. 29.4%, p=0.161). CONCLUSION: The MSU procedure has the advantage of reducing costs, time and mismatches, while ensuring comparable, and in some cases, better results than DG treatments. This technique can therefore be used as an alternative method to other conventional semen treatments.
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spelling pubmed-54737002017-06-27 Comparison of in vitro fertilization outcomes in ICSI cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation Palini, Simone Stefani, Silvia De Primiterra, Mariangela Benedetti, Serena Barone, Stefano Carli, Luca Vaccari, Enrico Murat, Ulug Feichtinger, Wilfried JBRA Assist Reprod Original Article OBJECTIVE: The aim of this study was to evaluate the efficacy of a non-expensive, easy and fast technique (direct micro swim-up) for sperm preparation in intracytoplasmic sperm injection (ICSI) treatments without the use of centrifuge. METHODS: We carried out a multicentric study in which a total of 140 ICSI-cycles were included. Sibling oocytes were divided into two groups according to semen preparation procedures: group A, discontinuous gradients (DG) (oocytes n=668), and group B, direct micro swim-up (MSU) (oocytes n=660). We analyzed differences in some key performance indicators. RESULTS: Fertilization rates were not statistically different between the DG and MSU groups (76.0% vs. 81.8%, respectively, p=0.248); while significant differences were found in blastulation rates per fertilized oocytes (41.7% vs. 58.5%, p=0.009), blastulation rates per D3 embryos (46.1% vs. 63.7%, p=0.045), and pregnancy rates (25.8% vs. 41.9%, p=0.045). The abortion rate was reduced in the MSU group as compared to DG, but not in a significant manner (12.9% vs. 29.4%, p=0.161). CONCLUSION: The MSU procedure has the advantage of reducing costs, time and mismatches, while ensuring comparable, and in some cases, better results than DG treatments. This technique can therefore be used as an alternative method to other conventional semen treatments. Brazilian Society of Assisted Reproduction 2017 /pmc/articles/PMC5473700/ /pubmed/28609274 http://dx.doi.org/10.5935/1518-0557.20170022 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Palini, Simone
Stefani, Silvia De
Primiterra, Mariangela
Benedetti, Serena
Barone, Stefano
Carli, Luca
Vaccari, Enrico
Murat, Ulug
Feichtinger, Wilfried
Comparison of in vitro fertilization outcomes in ICSI cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation
title Comparison of in vitro fertilization outcomes in ICSI cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation
title_full Comparison of in vitro fertilization outcomes in ICSI cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation
title_fullStr Comparison of in vitro fertilization outcomes in ICSI cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation
title_full_unstemmed Comparison of in vitro fertilization outcomes in ICSI cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation
title_short Comparison of in vitro fertilization outcomes in ICSI cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation
title_sort comparison of in vitro fertilization outcomes in icsi cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473700/
https://www.ncbi.nlm.nih.gov/pubmed/28609274
http://dx.doi.org/10.5935/1518-0557.20170022
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