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Whole-Genome Sequence of the Metastatic PC3 and LNCaP Human Prostate Cancer Cell Lines
The bone metastasis-derived PC3 and the lymph node metastasis-derived LNCaP prostate cancer cell lines are widely studied, having been described in thousands of publications over the last four decades. Here, we report short-read whole-genome sequencing (WGS) and de novo assembly of PC3 (ATCC CRL-143...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Genetics Society of America
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473753/ https://www.ncbi.nlm.nih.gov/pubmed/28413162 http://dx.doi.org/10.1534/g3.117.039909 |
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author | Seim, Inge Jeffery, Penny L. Thomas, Patrick B. Nelson, Colleen C. Chopin, Lisa K. |
author_facet | Seim, Inge Jeffery, Penny L. Thomas, Patrick B. Nelson, Colleen C. Chopin, Lisa K. |
author_sort | Seim, Inge |
collection | PubMed |
description | The bone metastasis-derived PC3 and the lymph node metastasis-derived LNCaP prostate cancer cell lines are widely studied, having been described in thousands of publications over the last four decades. Here, we report short-read whole-genome sequencing (WGS) and de novo assembly of PC3 (ATCC CRL-1435) and LNCaP (clone FGC; ATCC CRL-1740) at ∼70 × coverage. A known homozygous mutation in TP53 and homozygous loss of PTEN were robustly identified in the PC3 cell line, whereas the LNCaP cell line exhibited a larger number of putative inactivating somatic point and indel mutations (and in particular a loss of stop codon events). This study also provides preliminary evidence that loss of one or both copies of the tumor suppressor Capicua (CIC) contributes to primary tumor relapse and metastatic progression, potentially offering a treatment target for castration-resistant prostate cancer (CRPC). Our work provides a resource for genetic, genomic, and biological studies employing two commonly-used prostate cancer cell lines. |
format | Online Article Text |
id | pubmed-5473753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-54737532017-06-27 Whole-Genome Sequence of the Metastatic PC3 and LNCaP Human Prostate Cancer Cell Lines Seim, Inge Jeffery, Penny L. Thomas, Patrick B. Nelson, Colleen C. Chopin, Lisa K. G3 (Bethesda) Genome Report The bone metastasis-derived PC3 and the lymph node metastasis-derived LNCaP prostate cancer cell lines are widely studied, having been described in thousands of publications over the last four decades. Here, we report short-read whole-genome sequencing (WGS) and de novo assembly of PC3 (ATCC CRL-1435) and LNCaP (clone FGC; ATCC CRL-1740) at ∼70 × coverage. A known homozygous mutation in TP53 and homozygous loss of PTEN were robustly identified in the PC3 cell line, whereas the LNCaP cell line exhibited a larger number of putative inactivating somatic point and indel mutations (and in particular a loss of stop codon events). This study also provides preliminary evidence that loss of one or both copies of the tumor suppressor Capicua (CIC) contributes to primary tumor relapse and metastatic progression, potentially offering a treatment target for castration-resistant prostate cancer (CRPC). Our work provides a resource for genetic, genomic, and biological studies employing two commonly-used prostate cancer cell lines. Genetics Society of America 2017-04-14 /pmc/articles/PMC5473753/ /pubmed/28413162 http://dx.doi.org/10.1534/g3.117.039909 Text en Copyright © 2017 Seim et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Genome Report Seim, Inge Jeffery, Penny L. Thomas, Patrick B. Nelson, Colleen C. Chopin, Lisa K. Whole-Genome Sequence of the Metastatic PC3 and LNCaP Human Prostate Cancer Cell Lines |
title | Whole-Genome Sequence of the Metastatic PC3 and LNCaP Human Prostate Cancer Cell Lines |
title_full | Whole-Genome Sequence of the Metastatic PC3 and LNCaP Human Prostate Cancer Cell Lines |
title_fullStr | Whole-Genome Sequence of the Metastatic PC3 and LNCaP Human Prostate Cancer Cell Lines |
title_full_unstemmed | Whole-Genome Sequence of the Metastatic PC3 and LNCaP Human Prostate Cancer Cell Lines |
title_short | Whole-Genome Sequence of the Metastatic PC3 and LNCaP Human Prostate Cancer Cell Lines |
title_sort | whole-genome sequence of the metastatic pc3 and lncap human prostate cancer cell lines |
topic | Genome Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473753/ https://www.ncbi.nlm.nih.gov/pubmed/28413162 http://dx.doi.org/10.1534/g3.117.039909 |
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