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Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2′-deoxyguanosine as a Putative Biomarker
Coronary artery bypass grafting (CABG), one of the most common cardiac surgical procedures, is characterized by a burst of oxidative stress. 8-Oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), produced following DNA repairing, is used as an indicator of oxidative DNA damage in humans. The effect of CABG...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5474244/ https://www.ncbi.nlm.nih.gov/pubmed/28660009 http://dx.doi.org/10.1155/2017/9715898 |
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author | Turnu, Linda Di Minno, Alessandro Porro, Benedetta Squellerio, Isabella Bonomi, Alice Manega, Chiara Maria Werba, José Pablo Parolari, Alessandro Tremoli, Elena Cavalca, Viviana |
author_facet | Turnu, Linda Di Minno, Alessandro Porro, Benedetta Squellerio, Isabella Bonomi, Alice Manega, Chiara Maria Werba, José Pablo Parolari, Alessandro Tremoli, Elena Cavalca, Viviana |
author_sort | Turnu, Linda |
collection | PubMed |
description | Coronary artery bypass grafting (CABG), one of the most common cardiac surgical procedures, is characterized by a burst of oxidative stress. 8-Oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), produced following DNA repairing, is used as an indicator of oxidative DNA damage in humans. The effect of CABG on oxidative-induced DNA damage, evaluated through the measurement of urinary 8-oxodG by a developed and validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method in 52 coronary artery disease (CAD) patients, was assessed before (T0), five days (T1), and six months (T2) after CABG procedure. These results were compared with those obtained in 40 subjects with cardiovascular risk factors and without overt cardiovascular disease (CTR). Baseline (T0) 8-oxodG was higher in CAD than in CTR (p = 0.035). A significant burst was detected at T1 (p = 0.019), while at T2, 8-oxodG levels were significantly lower than those measured at T0 (p < 0.0001) and comparable to those found in CTR (p = 0.73). A similar trend was observed for urinary 8-iso-prostaglandin F(2α) (8-isoPGF(2α)), a reliable marker of oxidative stress. In the whole population baseline, 8-oxodG significantly correlated with 8-isoPGF(2α) levels (r = 0.323, p = 0.002). These data argue for CABG procedure in CAD patients as inducing a short-term increase in oxidative DNA damage, as revealed by 8-oxodG concentrations, and a long-term return of such metabolite toward physiological levels. |
format | Online Article Text |
id | pubmed-5474244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-54742442017-06-28 Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2′-deoxyguanosine as a Putative Biomarker Turnu, Linda Di Minno, Alessandro Porro, Benedetta Squellerio, Isabella Bonomi, Alice Manega, Chiara Maria Werba, José Pablo Parolari, Alessandro Tremoli, Elena Cavalca, Viviana Oxid Med Cell Longev Research Article Coronary artery bypass grafting (CABG), one of the most common cardiac surgical procedures, is characterized by a burst of oxidative stress. 8-Oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), produced following DNA repairing, is used as an indicator of oxidative DNA damage in humans. The effect of CABG on oxidative-induced DNA damage, evaluated through the measurement of urinary 8-oxodG by a developed and validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method in 52 coronary artery disease (CAD) patients, was assessed before (T0), five days (T1), and six months (T2) after CABG procedure. These results were compared with those obtained in 40 subjects with cardiovascular risk factors and without overt cardiovascular disease (CTR). Baseline (T0) 8-oxodG was higher in CAD than in CTR (p = 0.035). A significant burst was detected at T1 (p = 0.019), while at T2, 8-oxodG levels were significantly lower than those measured at T0 (p < 0.0001) and comparable to those found in CTR (p = 0.73). A similar trend was observed for urinary 8-iso-prostaglandin F(2α) (8-isoPGF(2α)), a reliable marker of oxidative stress. In the whole population baseline, 8-oxodG significantly correlated with 8-isoPGF(2α) levels (r = 0.323, p = 0.002). These data argue for CABG procedure in CAD patients as inducing a short-term increase in oxidative DNA damage, as revealed by 8-oxodG concentrations, and a long-term return of such metabolite toward physiological levels. Hindawi 2017 2017-06-04 /pmc/articles/PMC5474244/ /pubmed/28660009 http://dx.doi.org/10.1155/2017/9715898 Text en Copyright © 2017 Linda Turnu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Turnu, Linda Di Minno, Alessandro Porro, Benedetta Squellerio, Isabella Bonomi, Alice Manega, Chiara Maria Werba, José Pablo Parolari, Alessandro Tremoli, Elena Cavalca, Viviana Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2′-deoxyguanosine as a Putative Biomarker |
title | Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2′-deoxyguanosine as a Putative Biomarker |
title_full | Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2′-deoxyguanosine as a Putative Biomarker |
title_fullStr | Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2′-deoxyguanosine as a Putative Biomarker |
title_full_unstemmed | Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2′-deoxyguanosine as a Putative Biomarker |
title_short | Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2′-deoxyguanosine as a Putative Biomarker |
title_sort | assessing free-radical-mediated dna damage during cardiac surgery: 8-oxo-7,8-dihydro-2′-deoxyguanosine as a putative biomarker |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5474244/ https://www.ncbi.nlm.nih.gov/pubmed/28660009 http://dx.doi.org/10.1155/2017/9715898 |
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