Cargando…

Antihepatocarcinoma Effect of Portulaca oleracea L. in Mice by PI3K/Akt/mTOR and Nrf2/HO-1/NF-κB Pathway

The purpose of the present study was to evaluate the pharmacological effects of Portulaca oleracea L. (Purslane) (PL) on N-nitrosodiethylamine- (NDEA-) induced hepatocellular carcinomas (HCC) and explore its potential mechanism. Mice were randomly assigned to four groups: control group, NDEA group,...

Descripción completa

Detalles Bibliográficos
Autores principales: Guoyin, Zheng, Hao, Peng, Min, Li, Wei, Gu, Zhe, Chen, Changquan, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5474246/
https://www.ncbi.nlm.nih.gov/pubmed/28659990
http://dx.doi.org/10.1155/2017/8231358
_version_ 1783244411836039168
author Guoyin, Zheng
Hao, Peng
Min, Li
Wei, Gu
Zhe, Chen
Changquan, Ling
author_facet Guoyin, Zheng
Hao, Peng
Min, Li
Wei, Gu
Zhe, Chen
Changquan, Ling
author_sort Guoyin, Zheng
collection PubMed
description The purpose of the present study was to evaluate the pharmacological effects of Portulaca oleracea L. (Purslane) (PL) on N-nitrosodiethylamine- (NDEA-) induced hepatocellular carcinomas (HCC) and explore its potential mechanism. Mice were randomly assigned to four groups: control group, NDEA group, NDEA + Purslane (100 mg/kg) group, and NDEA + Purslane (200 mg/kg) group. The animal of each group was given NDEA (100 ppm) in drinking water. 1 h later, Purslane dissolved in PBS was intragastrically administered for continuous seven days. The results showed that Purslane reduced the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in liver and serum. Purslane also reduced the contents of interleukin-6 (IL-6), IL-1β, tumor necrosis factor-α (TNF-α), and methane dicarboxylic aldehyde (MDA) and restored the activity of superoxygen dehydrogenises (SOD) in serum. Purslane could obviously attenuate the hepatic pathological alteration. Furthermore, treatment with Purslane effectively inhibited the phosphorylations of phosphatidylinositol 3 kinase (PI3K), protein kinase B (Akt), mammalian target of rapamycin (mTOR), nuclear factor-kappa B (NF-κB), and inhibitor of NF-κBα (IκBα) and upregulated the expressions of NF-E2-related factor 2 (Nrf2) and heme oxygenase- (HO-) 1. In conclusion, our research suggested that Purslane exhibited protective effects on NDEA-induced hepatocellular carcinomas by anti-inflammatory and antioxidative properties via the PI3K/Akt/mTOR and Nrf2/HO-1/NF-κB pathway.
format Online
Article
Text
id pubmed-5474246
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-54742462017-06-28 Antihepatocarcinoma Effect of Portulaca oleracea L. in Mice by PI3K/Akt/mTOR and Nrf2/HO-1/NF-κB Pathway Guoyin, Zheng Hao, Peng Min, Li Wei, Gu Zhe, Chen Changquan, Ling Evid Based Complement Alternat Med Research Article The purpose of the present study was to evaluate the pharmacological effects of Portulaca oleracea L. (Purslane) (PL) on N-nitrosodiethylamine- (NDEA-) induced hepatocellular carcinomas (HCC) and explore its potential mechanism. Mice were randomly assigned to four groups: control group, NDEA group, NDEA + Purslane (100 mg/kg) group, and NDEA + Purslane (200 mg/kg) group. The animal of each group was given NDEA (100 ppm) in drinking water. 1 h later, Purslane dissolved in PBS was intragastrically administered for continuous seven days. The results showed that Purslane reduced the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in liver and serum. Purslane also reduced the contents of interleukin-6 (IL-6), IL-1β, tumor necrosis factor-α (TNF-α), and methane dicarboxylic aldehyde (MDA) and restored the activity of superoxygen dehydrogenises (SOD) in serum. Purslane could obviously attenuate the hepatic pathological alteration. Furthermore, treatment with Purslane effectively inhibited the phosphorylations of phosphatidylinositol 3 kinase (PI3K), protein kinase B (Akt), mammalian target of rapamycin (mTOR), nuclear factor-kappa B (NF-κB), and inhibitor of NF-κBα (IκBα) and upregulated the expressions of NF-E2-related factor 2 (Nrf2) and heme oxygenase- (HO-) 1. In conclusion, our research suggested that Purslane exhibited protective effects on NDEA-induced hepatocellular carcinomas by anti-inflammatory and antioxidative properties via the PI3K/Akt/mTOR and Nrf2/HO-1/NF-κB pathway. Hindawi 2017 2017-06-04 /pmc/articles/PMC5474246/ /pubmed/28659990 http://dx.doi.org/10.1155/2017/8231358 Text en Copyright © 2017 Zheng Guoyin et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Guoyin, Zheng
Hao, Peng
Min, Li
Wei, Gu
Zhe, Chen
Changquan, Ling
Antihepatocarcinoma Effect of Portulaca oleracea L. in Mice by PI3K/Akt/mTOR and Nrf2/HO-1/NF-κB Pathway
title Antihepatocarcinoma Effect of Portulaca oleracea L. in Mice by PI3K/Akt/mTOR and Nrf2/HO-1/NF-κB Pathway
title_full Antihepatocarcinoma Effect of Portulaca oleracea L. in Mice by PI3K/Akt/mTOR and Nrf2/HO-1/NF-κB Pathway
title_fullStr Antihepatocarcinoma Effect of Portulaca oleracea L. in Mice by PI3K/Akt/mTOR and Nrf2/HO-1/NF-κB Pathway
title_full_unstemmed Antihepatocarcinoma Effect of Portulaca oleracea L. in Mice by PI3K/Akt/mTOR and Nrf2/HO-1/NF-κB Pathway
title_short Antihepatocarcinoma Effect of Portulaca oleracea L. in Mice by PI3K/Akt/mTOR and Nrf2/HO-1/NF-κB Pathway
title_sort antihepatocarcinoma effect of portulaca oleracea l. in mice by pi3k/akt/mtor and nrf2/ho-1/nf-κb pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5474246/
https://www.ncbi.nlm.nih.gov/pubmed/28659990
http://dx.doi.org/10.1155/2017/8231358
work_keys_str_mv AT guoyinzheng antihepatocarcinomaeffectofportulacaoleracealinmicebypi3kaktmtorandnrf2ho1nfkbpathway
AT haopeng antihepatocarcinomaeffectofportulacaoleracealinmicebypi3kaktmtorandnrf2ho1nfkbpathway
AT minli antihepatocarcinomaeffectofportulacaoleracealinmicebypi3kaktmtorandnrf2ho1nfkbpathway
AT weigu antihepatocarcinomaeffectofportulacaoleracealinmicebypi3kaktmtorandnrf2ho1nfkbpathway
AT zhechen antihepatocarcinomaeffectofportulacaoleracealinmicebypi3kaktmtorandnrf2ho1nfkbpathway
AT changquanling antihepatocarcinomaeffectofportulacaoleracealinmicebypi3kaktmtorandnrf2ho1nfkbpathway