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In vitro physiological and antibacterial characterization of ZnO nanoparticle composites in simulated porcine gastric and enteric fluids

BACKGROUND: Diarrhea in piglets is one of the main causes of animal death after weaning; zinc oxide (ZnO) has been used in high doses for the control of this sickness. The aim of this study was to determine the physicochemical properties of ZnO nanoparticles synthesized and immobilized on a chitosan...

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Autores principales: Barreto, Marina S.R., Andrade, Cristina T., da Silva, Luiz Cláudio R. P., Cabral, Lúcio M., Flosi Paschoalin, Vânia M., Del Aguila, Eduardo M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5474296/
https://www.ncbi.nlm.nih.gov/pubmed/28623924
http://dx.doi.org/10.1186/s12917-017-1101-9
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author Barreto, Marina S.R.
Andrade, Cristina T.
da Silva, Luiz Cláudio R. P.
Cabral, Lúcio M.
Flosi Paschoalin, Vânia M.
Del Aguila, Eduardo M.
author_facet Barreto, Marina S.R.
Andrade, Cristina T.
da Silva, Luiz Cláudio R. P.
Cabral, Lúcio M.
Flosi Paschoalin, Vânia M.
Del Aguila, Eduardo M.
author_sort Barreto, Marina S.R.
collection PubMed
description BACKGROUND: Diarrhea in piglets is one of the main causes of animal death after weaning; zinc oxide (ZnO) has been used in high doses for the control of this sickness. The aim of this study was to determine the physicochemical properties of ZnO nanoparticles synthesized and immobilized on a chitosan/alginate (CH/SA) complex and investigate the antimicrobial activity and in vitro release profile of zinc (Zn(2+)) from these new compounds. The ZnO nanoparticles composites were prepared and combined with CH/SA or CH/SA and sodium tripolyphosphate (TPP). The structure and morphology of the composites were analyzed by characterization methods such as X-ray diffraction, FTIR spectroscopy, thermogravimetric analysis, atomic absorption spectrophotometry and scanning electron microscopy. RESULTS: The crystallite size of ZnO nano was 17 nm and the novel ZnO composites were effective in protecting ZnO in simulated gastric fluid, where Zn(2+) reached a concentration six-fold higher than the levels obtained with the unprotected commercial-zinc oxide. In addition, the novel composites suggest effective antimicrobial activity against Escherichia coli and Staphylococcus aureus. CONCLUSIONS: The results described herein suggest that the novel nano composites may work as an alternative product for pig feeding as verified by the in vitro assays, and may also contribute to lower the zinc released in the environment by fecal excretion in animals waste.
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spelling pubmed-54742962017-06-21 In vitro physiological and antibacterial characterization of ZnO nanoparticle composites in simulated porcine gastric and enteric fluids Barreto, Marina S.R. Andrade, Cristina T. da Silva, Luiz Cláudio R. P. Cabral, Lúcio M. Flosi Paschoalin, Vânia M. Del Aguila, Eduardo M. BMC Vet Res Research Article BACKGROUND: Diarrhea in piglets is one of the main causes of animal death after weaning; zinc oxide (ZnO) has been used in high doses for the control of this sickness. The aim of this study was to determine the physicochemical properties of ZnO nanoparticles synthesized and immobilized on a chitosan/alginate (CH/SA) complex and investigate the antimicrobial activity and in vitro release profile of zinc (Zn(2+)) from these new compounds. The ZnO nanoparticles composites were prepared and combined with CH/SA or CH/SA and sodium tripolyphosphate (TPP). The structure and morphology of the composites were analyzed by characterization methods such as X-ray diffraction, FTIR spectroscopy, thermogravimetric analysis, atomic absorption spectrophotometry and scanning electron microscopy. RESULTS: The crystallite size of ZnO nano was 17 nm and the novel ZnO composites were effective in protecting ZnO in simulated gastric fluid, where Zn(2+) reached a concentration six-fold higher than the levels obtained with the unprotected commercial-zinc oxide. In addition, the novel composites suggest effective antimicrobial activity against Escherichia coli and Staphylococcus aureus. CONCLUSIONS: The results described herein suggest that the novel nano composites may work as an alternative product for pig feeding as verified by the in vitro assays, and may also contribute to lower the zinc released in the environment by fecal excretion in animals waste. BioMed Central 2017-06-17 /pmc/articles/PMC5474296/ /pubmed/28623924 http://dx.doi.org/10.1186/s12917-017-1101-9 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Barreto, Marina S.R.
Andrade, Cristina T.
da Silva, Luiz Cláudio R. P.
Cabral, Lúcio M.
Flosi Paschoalin, Vânia M.
Del Aguila, Eduardo M.
In vitro physiological and antibacterial characterization of ZnO nanoparticle composites in simulated porcine gastric and enteric fluids
title In vitro physiological and antibacterial characterization of ZnO nanoparticle composites in simulated porcine gastric and enteric fluids
title_full In vitro physiological and antibacterial characterization of ZnO nanoparticle composites in simulated porcine gastric and enteric fluids
title_fullStr In vitro physiological and antibacterial characterization of ZnO nanoparticle composites in simulated porcine gastric and enteric fluids
title_full_unstemmed In vitro physiological and antibacterial characterization of ZnO nanoparticle composites in simulated porcine gastric and enteric fluids
title_short In vitro physiological and antibacterial characterization of ZnO nanoparticle composites in simulated porcine gastric and enteric fluids
title_sort in vitro physiological and antibacterial characterization of zno nanoparticle composites in simulated porcine gastric and enteric fluids
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5474296/
https://www.ncbi.nlm.nih.gov/pubmed/28623924
http://dx.doi.org/10.1186/s12917-017-1101-9
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