Q&A: Expansion microscopy

Expansion microscopy (ExM) is a recently invented technology that uses swellable charged polymers, synthesized densely and with appropriate topology throughout a preserved biological specimen, to physically magnify the specimen 100-fold in volume, or more, in an isotropic fashion. ExM enables nanosc...

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Detalles Bibliográficos
Autores principales: Gao, Ruixuan, Asano, Shoh M., Boyden, Edward S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Question and Answer
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5474863/
https://www.ncbi.nlm.nih.gov/pubmed/28629474
http://dx.doi.org/10.1186/s12915-017-0393-3
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author Gao, Ruixuan
Asano, Shoh M.
Boyden, Edward S.
author_facet Gao, Ruixuan
Asano, Shoh M.
Boyden, Edward S.
author_sort Gao, Ruixuan
collection PubMed
description Expansion microscopy (ExM) is a recently invented technology that uses swellable charged polymers, synthesized densely and with appropriate topology throughout a preserved biological specimen, to physically magnify the specimen 100-fold in volume, or more, in an isotropic fashion. ExM enables nanoscale resolution imaging of preserved samples on inexpensive, fast, conventional microscopes. How does ExM work? How good is its performance? How do you get going on using it? In this Q&A, we provide the answers to these and other questions about this new and rapidly spreading toolbox.
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spelling pubmed-54748632017-06-21 Q&A: Expansion microscopy Gao, Ruixuan Asano, Shoh M. Boyden, Edward S. BMC Biol Question and Answer Expansion microscopy (ExM) is a recently invented technology that uses swellable charged polymers, synthesized densely and with appropriate topology throughout a preserved biological specimen, to physically magnify the specimen 100-fold in volume, or more, in an isotropic fashion. ExM enables nanoscale resolution imaging of preserved samples on inexpensive, fast, conventional microscopes. How does ExM work? How good is its performance? How do you get going on using it? In this Q&A, we provide the answers to these and other questions about this new and rapidly spreading toolbox. BioMed Central 2017-06-19 /pmc/articles/PMC5474863/ /pubmed/28629474 http://dx.doi.org/10.1186/s12915-017-0393-3 Text en © Boyden et al. 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Question and Answer
Gao, Ruixuan
Asano, Shoh M.
Boyden, Edward S.
Q&A: Expansion microscopy
title Q&A: Expansion microscopy
title_full Q&A: Expansion microscopy
title_fullStr Q&A: Expansion microscopy
title_full_unstemmed Q&A: Expansion microscopy
title_short Q&A: Expansion microscopy
title_sort q&a: expansion microscopy
topic Question and Answer
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5474863/
https://www.ncbi.nlm.nih.gov/pubmed/28629474
http://dx.doi.org/10.1186/s12915-017-0393-3
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