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Effects of diesel exhaust particle exposure on a murine model of asthma due to soybean
BACKGROUND: Exposure to soybean allergens has been linked to asthma outbreaks. Exposure to diesel exhaust particles (DEP) has been associated with an increase in the risk of asthma and asthma exacerbation; however, in both cases the underlying mechanisms remain poorly understood, as does the possibl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476280/ https://www.ncbi.nlm.nih.gov/pubmed/28628664 http://dx.doi.org/10.1371/journal.pone.0179569 |
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author | Alvarez-Simón, Daniel Muñoz, Xavier Gómez-Ollés, Susana de Homdedeu, Miquel Untoria, María-Dolores Cruz, María-Jesús |
author_facet | Alvarez-Simón, Daniel Muñoz, Xavier Gómez-Ollés, Susana de Homdedeu, Miquel Untoria, María-Dolores Cruz, María-Jesús |
author_sort | Alvarez-Simón, Daniel |
collection | PubMed |
description | BACKGROUND: Exposure to soybean allergens has been linked to asthma outbreaks. Exposure to diesel exhaust particles (DEP) has been associated with an increase in the risk of asthma and asthma exacerbation; however, in both cases the underlying mechanisms remain poorly understood, as does the possible interaction between the two entities. OBJECTIVE: To investigate how the combination of soybean allergens and DEP can affect the induction or exacerbation of asthma in a murine model. METHODS: BALB/c mice received intranasal instillations of saline, 3 or 5 mg protein/ml soybean hull extract (SHE), or a combination of one of these three solutions with DEP. Airway hyperresponsiveness (AHR), pulmonary inflammation in bronchoalveolar lavage, total serum immunoglobulin E and histological studies were assessed. RESULTS: A 5 mg protein/ml SHE solution was able by itself to enhance AHR (p = 0.0033), increase eosinophilic inflammation (p = 0.0003), increase levels of IL-4, IL-5, IL-13, IL-17A, IL-17F and CCL20, and reduce levels of IFN-γ. The combination of 5 mg protein/ml SHE with DEP also produced an increase in AHR and eosinophilic inflammation, but presented a slightly different cytokine profile with higher levels of Th17-related cytokines. However, while the 3 mg protein/ml SHE solution did not induce asthma, co-exposure with DEP resulted in a markedly enhanced AHR (p = 0.002) and eosinophilic inflammation (p = 0.004), with increased levels of IL-5, IL-17F and CCL20 and decreased levels of IFN-γ. CONCLUSIONS & CLINICAL RELEVANCE: The combination of soybean allergens and DEP is capable of triggering an asthmatic response through a Th17-related mechanism when the soybean allergen concentration is too low to promote a response by itself. DEP monitoring may be a useful addition to allergen monitoring in order to prevent new asthma outbreaks. |
format | Online Article Text |
id | pubmed-5476280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54762802017-07-03 Effects of diesel exhaust particle exposure on a murine model of asthma due to soybean Alvarez-Simón, Daniel Muñoz, Xavier Gómez-Ollés, Susana de Homdedeu, Miquel Untoria, María-Dolores Cruz, María-Jesús PLoS One Research Article BACKGROUND: Exposure to soybean allergens has been linked to asthma outbreaks. Exposure to diesel exhaust particles (DEP) has been associated with an increase in the risk of asthma and asthma exacerbation; however, in both cases the underlying mechanisms remain poorly understood, as does the possible interaction between the two entities. OBJECTIVE: To investigate how the combination of soybean allergens and DEP can affect the induction or exacerbation of asthma in a murine model. METHODS: BALB/c mice received intranasal instillations of saline, 3 or 5 mg protein/ml soybean hull extract (SHE), or a combination of one of these three solutions with DEP. Airway hyperresponsiveness (AHR), pulmonary inflammation in bronchoalveolar lavage, total serum immunoglobulin E and histological studies were assessed. RESULTS: A 5 mg protein/ml SHE solution was able by itself to enhance AHR (p = 0.0033), increase eosinophilic inflammation (p = 0.0003), increase levels of IL-4, IL-5, IL-13, IL-17A, IL-17F and CCL20, and reduce levels of IFN-γ. The combination of 5 mg protein/ml SHE with DEP also produced an increase in AHR and eosinophilic inflammation, but presented a slightly different cytokine profile with higher levels of Th17-related cytokines. However, while the 3 mg protein/ml SHE solution did not induce asthma, co-exposure with DEP resulted in a markedly enhanced AHR (p = 0.002) and eosinophilic inflammation (p = 0.004), with increased levels of IL-5, IL-17F and CCL20 and decreased levels of IFN-γ. CONCLUSIONS & CLINICAL RELEVANCE: The combination of soybean allergens and DEP is capable of triggering an asthmatic response through a Th17-related mechanism when the soybean allergen concentration is too low to promote a response by itself. DEP monitoring may be a useful addition to allergen monitoring in order to prevent new asthma outbreaks. Public Library of Science 2017-06-19 /pmc/articles/PMC5476280/ /pubmed/28628664 http://dx.doi.org/10.1371/journal.pone.0179569 Text en © 2017 Alvarez-Simón et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Alvarez-Simón, Daniel Muñoz, Xavier Gómez-Ollés, Susana de Homdedeu, Miquel Untoria, María-Dolores Cruz, María-Jesús Effects of diesel exhaust particle exposure on a murine model of asthma due to soybean |
title | Effects of diesel exhaust particle exposure on a murine model of asthma due to soybean |
title_full | Effects of diesel exhaust particle exposure on a murine model of asthma due to soybean |
title_fullStr | Effects of diesel exhaust particle exposure on a murine model of asthma due to soybean |
title_full_unstemmed | Effects of diesel exhaust particle exposure on a murine model of asthma due to soybean |
title_short | Effects of diesel exhaust particle exposure on a murine model of asthma due to soybean |
title_sort | effects of diesel exhaust particle exposure on a murine model of asthma due to soybean |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476280/ https://www.ncbi.nlm.nih.gov/pubmed/28628664 http://dx.doi.org/10.1371/journal.pone.0179569 |
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