Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining

Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectroscopy (TER...

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Autores principales: Berezin, Shirly, Aviv, Yaron, Aviv, Hagit, Goldberg, Elad, Tischler, Yaakov R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476599/
https://www.ncbi.nlm.nih.gov/pubmed/28630418
http://dx.doi.org/10.1038/s41598-017-02212-2
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author Berezin, Shirly
Aviv, Yaron
Aviv, Hagit
Goldberg, Elad
Tischler, Yaakov R.
author_facet Berezin, Shirly
Aviv, Yaron
Aviv, Hagit
Goldberg, Elad
Tischler, Yaakov R.
author_sort Berezin, Shirly
collection PubMed
description Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectroscopy (TERS) can differentiate between Gram negative and positive species, by detecting the changes in tip-enhancement in the Raman scattering from the bacteria’s lipid-bilayer membrane, which specifically enhances Gram negative bacteria.
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spelling pubmed-54765992017-06-23 Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining Berezin, Shirly Aviv, Yaron Aviv, Hagit Goldberg, Elad Tischler, Yaakov R. Sci Rep Article Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectroscopy (TERS) can differentiate between Gram negative and positive species, by detecting the changes in tip-enhancement in the Raman scattering from the bacteria’s lipid-bilayer membrane, which specifically enhances Gram negative bacteria. Nature Publishing Group UK 2017-06-19 /pmc/articles/PMC5476599/ /pubmed/28630418 http://dx.doi.org/10.1038/s41598-017-02212-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Berezin, Shirly
Aviv, Yaron
Aviv, Hagit
Goldberg, Elad
Tischler, Yaakov R.
Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining
title Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining
title_full Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining
title_fullStr Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining
title_full_unstemmed Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining
title_short Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining
title_sort replacing a century old technique – modern spectroscopy can supplant gram staining
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476599/
https://www.ncbi.nlm.nih.gov/pubmed/28630418
http://dx.doi.org/10.1038/s41598-017-02212-2
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