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The Arbuscular Mycorrhizal Fungus Funneliformis mosseae Alters Bacterial Communities in Subtropical Forest Soils during Litter Decomposition

Bacterial communities and arbuscular mycorrhizal fungi (AMF) co-occur in the soil, however, the interaction between these two groups during litter decomposition remains largely unexplored. In order to investigate the effect of AMF on soil bacterial communities, we designed dual compartment microcosm...

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Autores principales: Gui, Heng, Purahong, Witoon, Hyde, Kevin D., Xu, Jianchu, Mortimer, Peter E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476864/
https://www.ncbi.nlm.nih.gov/pubmed/28676797
http://dx.doi.org/10.3389/fmicb.2017.01120
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author Gui, Heng
Purahong, Witoon
Hyde, Kevin D.
Xu, Jianchu
Mortimer, Peter E.
author_facet Gui, Heng
Purahong, Witoon
Hyde, Kevin D.
Xu, Jianchu
Mortimer, Peter E.
author_sort Gui, Heng
collection PubMed
description Bacterial communities and arbuscular mycorrhizal fungi (AMF) co-occur in the soil, however, the interaction between these two groups during litter decomposition remains largely unexplored. In order to investigate the effect of AMF on soil bacterial communities, we designed dual compartment microcosms, where AMF (Funneliformis mosseae) was allowed access (AM) to, or excluded (NM) from, a compartment containing forest soil and litterbags. Soil samples from this compartment were analyzed at 0, 90, 120, 150, and 180 days. For each sample, Illumina sequencing was used to assess any changes in the soil bacterial communities. We found that most of the obtained operational taxonomic units (OTUs) from both treatments belonged to the phylum of Proteobacteria, Acidobacteria, and Actinobacteria. The community composition of bacteria at phylum and class levels was slightly influenced by both time and AMF. In addition, time and AMF significantly affected bacterial genera (e.g., Candidatus Solibacter, Dyella, Phenylobacterium) involved in litter decomposition. Opposite to the bacterial community composition, we found that overall soil bacterial OTU richness and diversity are relatively stable and were not significantly influenced by either time or AMF inoculation. OTU richness at phylum and class levels also showed consistent results with overall bacterial OTU richness. Our study provides new insight into the influence of AMF on soil bacterial communities at the genus level.
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spelling pubmed-54768642017-07-04 The Arbuscular Mycorrhizal Fungus Funneliformis mosseae Alters Bacterial Communities in Subtropical Forest Soils during Litter Decomposition Gui, Heng Purahong, Witoon Hyde, Kevin D. Xu, Jianchu Mortimer, Peter E. Front Microbiol Microbiology Bacterial communities and arbuscular mycorrhizal fungi (AMF) co-occur in the soil, however, the interaction between these two groups during litter decomposition remains largely unexplored. In order to investigate the effect of AMF on soil bacterial communities, we designed dual compartment microcosms, where AMF (Funneliformis mosseae) was allowed access (AM) to, or excluded (NM) from, a compartment containing forest soil and litterbags. Soil samples from this compartment were analyzed at 0, 90, 120, 150, and 180 days. For each sample, Illumina sequencing was used to assess any changes in the soil bacterial communities. We found that most of the obtained operational taxonomic units (OTUs) from both treatments belonged to the phylum of Proteobacteria, Acidobacteria, and Actinobacteria. The community composition of bacteria at phylum and class levels was slightly influenced by both time and AMF. In addition, time and AMF significantly affected bacterial genera (e.g., Candidatus Solibacter, Dyella, Phenylobacterium) involved in litter decomposition. Opposite to the bacterial community composition, we found that overall soil bacterial OTU richness and diversity are relatively stable and were not significantly influenced by either time or AMF inoculation. OTU richness at phylum and class levels also showed consistent results with overall bacterial OTU richness. Our study provides new insight into the influence of AMF on soil bacterial communities at the genus level. Frontiers Media S.A. 2017-06-20 /pmc/articles/PMC5476864/ /pubmed/28676797 http://dx.doi.org/10.3389/fmicb.2017.01120 Text en Copyright © 2017 Gui, Purahong, Hyde, Xu and Mortimer. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gui, Heng
Purahong, Witoon
Hyde, Kevin D.
Xu, Jianchu
Mortimer, Peter E.
The Arbuscular Mycorrhizal Fungus Funneliformis mosseae Alters Bacterial Communities in Subtropical Forest Soils during Litter Decomposition
title The Arbuscular Mycorrhizal Fungus Funneliformis mosseae Alters Bacterial Communities in Subtropical Forest Soils during Litter Decomposition
title_full The Arbuscular Mycorrhizal Fungus Funneliformis mosseae Alters Bacterial Communities in Subtropical Forest Soils during Litter Decomposition
title_fullStr The Arbuscular Mycorrhizal Fungus Funneliformis mosseae Alters Bacterial Communities in Subtropical Forest Soils during Litter Decomposition
title_full_unstemmed The Arbuscular Mycorrhizal Fungus Funneliformis mosseae Alters Bacterial Communities in Subtropical Forest Soils during Litter Decomposition
title_short The Arbuscular Mycorrhizal Fungus Funneliformis mosseae Alters Bacterial Communities in Subtropical Forest Soils during Litter Decomposition
title_sort arbuscular mycorrhizal fungus funneliformis mosseae alters bacterial communities in subtropical forest soils during litter decomposition
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476864/
https://www.ncbi.nlm.nih.gov/pubmed/28676797
http://dx.doi.org/10.3389/fmicb.2017.01120
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